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Recombinant ASS1 Antibody - Primary antibody, specific to ASS1, Rabbit IgG

COA COA
    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab089874
Grouped product items
SKU Size
Availability
 Price Qty
Ab089874-10μl
10μl
2
$66.90
Ab089874-50μl
50μl
1
$199.90
Ab089874-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$319.90
Ab089874-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti Human ASS1 Antibody; WB, IHC, ICC, IF; Unconjugated

View related series
Click to view target information: ASS1
Accession#:P00966 (1) ASS1 (1) Gene ID:445 (1)

Basic Description

Product Name Recombinant ASS1 Antibody - Primary antibody, specific to ASS1, Rabbit IgG
Synonyms Argininosuccinate synthase 1 antibody | Argininosuccinate synthase antibody | Argininosuccinate synthetase 1 antibody | ASSantibody | Ass-1 antibody | ass1 antibody | ASSA antibody | ASSY_HUMAN antibody | Citrulline aspartate ligase antibody | Citrulline-
Specifications & Purity ExactAb™, Validated, Recombinant, 1.0 mg/mL
Host species Rabbit
Specificity ASS1
Immunogen A synthetic peptide derived from human ASS1 (AA 41-54)
Positive Control WB: A431 and HeLa cell lysates. IHC: Human breast cancer tissue. ICC/IF: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human ASS1 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Argininosuccinate synthase 1 (Ass1) can catalyze the formation of arginine succinate from citrulline and aspartic acid. Under the action of argininosuccinate lyase (Asl), arginine is produced. Arginine is hydrolyzed by arginase to produce urea and ornithine, which can be metabolized by ornithine decarboxylase to produce polyamines; It can also regenerate citrulline under the action of ornithine transaminase, forming a urea cycle; In addition, arginine can also generate nitric oxide (NO) and citrulline through the action of nitric oxide synthase (NOS), forming the citrulline nitric oxide cycle. Therefore, Ass1 is a key rate limiting enzyme for the production of arginine, urea, and NO.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Antigen affinity purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

ASS1 Tbio Argininosuccinate synthase (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant ASS1 Antibody (Ab089874) - Western Blot
All lanes: Recombinant ASS1 Antibody (Ab089874) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 47 kDa
Observed band size: 44 kDa
Exposure time: 27.8 s

Recombinant ASS1 Antibody (Ab089874) - IF/ICC
Immunocytochemistry analysis of ASS1 (green) in HeLa using Recombinant ASS1 Antibody (Ab089874), and cells were counterstained with DAPI (blue).


Recombinant ASS1 Antibody (Ab089874) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant ASS1 Antibody (Ab089874). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application Dilution info
WB

1/500-1/1000
IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0303185 Certificate of Analysis Mar 13, 2024 Ab089874
ZJ24F0303184 Certificate of Analysis Mar 13, 2024 Ab089874

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