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MGMT Antibody - Primary antibody, specific to MGMT, Rabbit IgG

    Application:
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab115239
Grouped product items
SKU Size
Availability
Price Qty
Ab115239-10μl
10μl
1
$66.90
Ab115239-50μl
50μl
4
$199.90
Ab115239-100μl
100μl
3
$289.90
Ab115239-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

pAb; Rabbit anti Human MGMT Antibody; WB, IHC; Unconjugated

Basic Description

Product Name MGMT Antibody - Primary antibody, specific to MGMT, Rabbit IgG
Synonyms 6 O methylguanine DNA methyltransferase antibody | 6-O-methylguanine-DNA methyltransferase antibody | Agat antibody | AGT antibody | AI267024 antibody | EC 2.1.1.63 antibody | Methylated DNA protein cysteine methyltransferase antibody | Methylated-DNA--pr
Specifications & Purity Carrier Free, ExactAb™, Azide Free, Validated, See COA
Host species Rabbit
Specificity MGMT
Immunogen Recombinant MGMT expressed in E.coli (AA 1-207).
Positive Control WB: MCF7, Jurkat, HeLa, MOLT-4, HT-29 and Hep G2 cell lysate. IHC: Human stomach tissue.
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, Validated
Product Description

Rabbit anti Human MGMT Antibody, Polyclonal antibody, could be used for WB, IHC and so on.
Application
WB: 0.5-3µg/mL
IHC: 5-30µg/mL
Protein Function
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

MGMT Tchem Methylated-DNA--protein-cysteine methyltransferase (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
MGMT Tchem 6-O-methylguanine-DNA methyltransferase (451 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

MGMT Antibody (Ab115239) - Western Blot
All lanes: MGMT Antibody (Ab115239) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 21 kDa
Observed band size: 24 kDa
Exposure time: 2.6 s

MGMT Antibody (Ab115239) - IHC
DAB staining on IHC
Samples: Human Stomach Tissue
Primary Ab: 10 µg/mL MGMT Antibody (Ab115239)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal
Antibody.

Application

Application Dilution info
IHC

5-30 µg/mL

WB

0.5-3 µg/mL

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1101587 Certificate of Analysis Nov 08, 2023 Ab115239
ZJ23F1101586 Certificate of Analysis Nov 08, 2023 Ab115239

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