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Recombinant ERK2 Antibody - Primary antibody, specific to MAPK1, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab102233
Grouped product items
SKU Size
Availability
Price Qty
Ab102233-10μl
10μl
5
$59.90
Ab102233-50μl
50μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$189.90
Ab102233-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$289.90
Ab102233-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,299.90

Recombinant; Rabbit anti Human ERK2 Antibody; WB, IHC, IF, ICC; Unconjugated

Basic Description

Product Name Recombinant ERK2 Antibody - Primary antibody, specific to MAPK1, Rabbit IgG
Synonyms ERK 2 antibody | ERK-2 antibody | ERT1 antibody | Extracellular Signal Regulated Kinase 2 antibody | Extracellular signal-regulated kinase 2 antibody | MAP kinase 1 antibody | MAP kinase 2 antibody | MAP kinase isoform p42 antibody | MAPK 1 antibody | MAP
Specifications & Purity ExactAb™, Validated, Recombinant, 0.5 mg/mL
Host species Rabbit
Specificity MAPK1
Immunogen A synthetic peptide derived from human ERK2 (AA 340-360).
Positive Control WB: K562, C6, NIH/3T3, HeLa, MCF7, A549, Hep G2, HEK293 lysates. IHC: Human Cholangiocarcinoma Tissue. ICC/IF: K562 cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human ERK2 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/50-1/200
Protein Function
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2. Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Antigen affinity purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

MAPK1 Tchem Mitogen-activated protein kinase 1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
MAPK1 Tchem MAP kinase ERK2 (25055 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant ERK2 Antibody (Ab102233) - Western Blot
All lanes: Recombinant ERK2 Antibody (Ab102233) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 41 kDa
Observed band size: 39 kDa

Recombinant ERK2 Antibody (Ab102233) - ICC/IF
IF analysis of ERK2 (green) in K562 cells using Recombinant ERK2 Antibody (Ab102233) and cells were counterstained with DAPI (blue).

Recombinant ERK2 Antibody (Ab102233) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant ERK2 Antibody (Ab102233). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application Dilution info
WB

1/500-1/1000

IHC

1/50 - 1/100

IF/ICC

1/50 - 1/200

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0800761 Certificate of Analysis Aug 03, 2023 Ab102233
ZJ23F0800760 Certificate of Analysis Aug 03, 2023 Ab102233

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