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Recombinant Cytokeratin 20 Antibody - Primary antibody, specific to Cytokeratin 20, Rabbit IgG

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • IHC
  • IP
  • WB
  • Host species: Rabbit
  • Reactive species: Human
  • Isotype: Rabbit IgG
  • Clone Number: RR648
  • Conjugation: Unconjugated
In stock
Item Number
Ab098925
Grouped product items
SKU Size
Availability
 Price Qty
Ab098925-10μl
10μl
5
$69.90
Ab098925-50μl
50μl
1
$207.90
Ab098925-100μl
100μl
2
$327.90
Ab098925-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,397.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant (RR648); Rabbit anti Human Cytokeratin 20 Antibody; WB, IHC, ICC, IF, Flow, IP; Unconjugated

View related series
Click to view target information: KRT20
Accession#:P35900 (5) Cytokeratin 20 (5) Flow Cytometry (1158) Gene ID:54474 (5) KRT20 (5)

Basic Description

Product Name Recombinant Cytokeratin 20 Antibody - Primary antibody, specific to Cytokeratin 20, Rabbit IgG
Synonyms CD20 antibody | CK 20 antibody | CK-20 antibody | CK20 antibody | Cytokeratin-20 antibody | Cytokeratin20 antibody | K1C20_HUMAN antibody | K20 antibody | KA20 antibody | Keratin 20 antibody | keratin 20, type I antibody | keratin 21, rat, homolog of anti
Specifications & Purity ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL
Host species Rabbit
Specificity Cytokeratin 20
Immunogen A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 20.
Positive Control WB: HT-29 cell lysate. IHC: Colonic adenocarcinoma tissue. Flow: HT-29 cells. ICC/IF: HT-29 cells. IP: HT-29 whole cell lysate.
Conjugation Unconjugated
Grade ExactAb™, High Performance, Recombinant, Validated
Product Description

Rabbit anti Human Cytokeratin 20 Antibody, Recombinant antibody (RR648), could be used for WB, IHC, ICC/IF, Flow and IP.
Application:
WB: 1/5000–1/20000
IHC: 1/800-1/1600
ICC/IF: 1/50-1/2000
Flow: 1/10-1/1000
IP: 1/50
Protein Function
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

KRT20 Tbio Keratin, type I cytoskeletal 20 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant ACTA2 Antibody (Ab088446) - Western Blot
All lanes: Recombinant Cytokeratin 20 Antibody (Ab098925) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 49 kDa
Observed band size: 46 kDa
Exposure time: 0.1 s

Recombinant Cytokeratin 20 Antibody (Ab098925) - IF/ICC
Recombinant Cytokeratin 20 Antibody (Ab098925) staining Cytokeratin 20 in HT-29 cells by IF/ICC (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant Cytokeratin 20 Antibody (Ab098925) (1/200) at 4°C. An AF488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500).

Recombinant Cytokeratin 20 Antibody (Ab098925) - Flow Cytometry
Overlay histogram showing HT-29 cells stained with Recombinant Cytokeratin 20 Antibody (Ab098925) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant Cytokeratin 20 Antibody (Ab098925) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at 4°C. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at 4°C. Unlabeled sample (Blue) was used as a control.

Recombinant Cytokeratin 20 Antibody (Ab098925) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of colonic adenocarcinoma tissue labelling Cytokeratin 20 with Recombinant Cytokeratin 20 Antibody (Ab098925) at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Recombinant Cytokeratin 20 Antibody (Ab098925) - IP
Cytokeratin 20 was immunoprecipitated from 0.5 mg of HT-29 lysate with Recombinant Cytokeratin 20 Antibody (Ab098925) at 1/50 dilution.
2nd Ab: G&R HRP for IP 1/500

Lane 1: Recombinant Cytokeratin 20 Antibody (Ab098925) IP in HT-29 whole cell lysate
Lane 2: PBS instead of Recombinant Cytokeratin 20 Antibody (Ab098925) in HT-29 whole cell lysate
Lane 3: HT-29 whole cell lysate, 10 μg (input)

Exposure: 30.0 s.

Application

Application Dilution info
IP

1/50

WB

1/5000 - 1/20000

IHC

1/800 - 1/1600
Flow Cytometry

1/10-1/1000

IF/ICC

1/50-1/2000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1001219 Certificate of Analysis Oct 10, 2023 Ab098925
ZJ23F1001218 Certificate of Analysis Oct 10, 2023 Ab098925

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