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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab212580-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$79.90
|
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Ab212580-50μl
|
50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$229.90
|
|
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Ab212580-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$366.90
|
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Ab212580-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,999.90
|
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mAb (CD02/2H1); Mouse anti Human Cytokeratin 20; WB, IF/ICC, ELISA; Unconjugated
| Product Name | Cytokeratin 20 Mouse mAb - Primary antibody, specific to Human Cytokeratin 20 |
|---|---|
| Synonyms | CD20 antibody | CK 20 antibody | CK-20 antibody | CK20 antibody | Cytokeratin-20 antibody | Cytokeratin20 antibody | K1C20_HUMAN antibody | K20 antibody | KA20 antibody | Keratin 20 antibody | keratin 20 | keratin 21 | Keratin antibody | Keratin type I cy |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High Performance, ≥95%(SDS-PAGE), 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity). |
| Host species | Mouse |
| Specificity | Human Cytokeratin 20 |
| Immunogen | Recombinant protein corresponding to Human Cytokeratin 20 |
| Positive Control | WB: HT-29 whole cell lysates IF/ICC: MCF-7 cells |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Mouse anti Human Cytokeratin 20 Antibody, clone CD02/2H1, could be used for WB, IF/ICC, ELISA and so on. |
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150.0 KDa |
| Purification Method | Protein A purified |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Cytokeratin 20 Mouse mAb (Ab212580) - Western Blot
All lanes: Cytokeratin 20 Mouse Antibody (Ab212580) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 44,47 kDa
Exposure time: 10.0 s
Cytokeratin 20 Mouse mAb (Ab212580) - ICC/IF
IF analysis of Cytokeratin 20 (Green) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Cytokeratin 20 Mouse mAb (Ab212580) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| IF/ICC | 1/200 |
| WB |
1/1000 - 1/20000
|
Starting at $79.90
Starting at $69.90
Starting at $79.90