Lactate Dehydrogenase Mouse mAb

COA

级别和纯度:

≥95%(SDS-PAGE)
0.5 mg/mL

应用:

ELISA
IF/ICC
WB

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG1
克隆号: AB01/3H8
偶联: Unconjugated

有货

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货号 (SKU)	包装规格	是否现货
Ab156217-10μl	10μl	现货
Ab156217-50μl	50μL	现货
Ab156217-100μl	100μL	现货
Ab156217-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: LDHA

Accession#:P00338 (4) Gene ID:3939 (4) Lactate Dehydrogenase (32) LDHA (6)

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基本信息

产品名称	Lactate Dehydrogenase Mouse mAb
别名	乳酸脱氢酶A \| 乳酸脱氢酶A抗体 \| 乳酸脱氢酶A小鼠单克隆抗体 \| 小鼠抗人乳酸脱氢酶A抗体 \| Lactate Dehydrogenase抗体 \| LDHA抗体.
英文别名	Cell proliferation-inducing gene 19 protein antibody \| GSD11 antibody \| L lactate dehydrogenase B chain antibody \| L-lactate dehydrogenase A chain antibody \| Lactate dehydrogenase A antibody \| Lactate dehydrogenase B antibody \| Lactate dehydrogenase c var
规格或纯度	ExactAb™, 已验证, 无载体, 无叠氮钠, 高性能, ≥95%(SDS-PAGE), 0.5 mg/mL
生化机理	Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD+.
宿主种属	小鼠(Mouse)
特异性	Human LDHA
种属反应性	人(Human)
免疫原	Recombinant protein corresponding to Human LDHA
阳性对照	WB: HeLa, HepG2, A549 and A431 whole cell lysates
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein A purified
储存缓冲液	10mM PBS, 50% Glycerol, pH7.4
浓度	0.5 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4°C short term (1-2 weeks). Store at -20°C . Avoid freeze / thaw cycle.
分子类型	抗体

关联靶点（人）

LDHA Tchem L-乳酸脱氢酶A链(L-lactate dehydrogenase A chain) (0 活性数据)

点击查看靶蛋白信息： LDHA

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: Lactate Dehydrogenase Mouse mAb (Ab156217)

tate Dehydrogenase Mouse mAb (Ab156217) - Western Blot
All lanes: Lactate Dehydrogenase Mouse Antibody (Ab156217) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 36.7 kDa
Observed band size: 35.1 kDa
Exposure time: 60.0 s

ICC/IF: Lactate Dehydrogenase Mouse mAb (Ab156217)

Lactate Dehydrogenase Mouse mAb (Ab156217) - ICC/IF
IF analysis of Lactate Dehydrogenase (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Lactate Dehydrogenase Mouse mAb (Ab156217) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

应用