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Recombinant Lactate Dehydrogenase Antibody

Recombinant Lactate Dehydrogenase Antibody

级别和纯度:

见COA

应用:

IF/ICC
IHC
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
亚型: Rabbit IgG
偶联: Unconjugated

有货

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货号 (SKU)	包装规格	是否现货
Ab112519-10μl	10μl	现货
Ab112519-50μl	50μL	现货
Ab112519-100μl	100μL	现货
Ab112519-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: CEND1 点击查看靶蛋白信息: LDHA

Accession#:P00338 (4) Gene ID:3939 (4) Lactate Dehydrogenase (32) LDHA (6)

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基本信息

产品名称	Recombinant Lactate Dehydrogenase Antibody
别名	乳酸脱氢酶A抗体 \| 乳酸脱氢酶A单克隆抗体 \| 乳酸脱氢酶A重组单克隆抗体 \| Lactate Dehydrogenase抗体 \| Lactate Dehydrogenase单克隆抗体 \| Lactate Dehydrogenase重组单克隆抗体 \| LDHA抗体 \| LDHA单克隆抗体 \| LDHA重组单克隆抗体
英文别名	Cell proliferation-inducing gene 19 protein antibody \| GSD11 antibody \| L lactate dehydrogenase B chain antibody \| L-lactate dehydrogenase A chain antibody \| Lactate dehydrogenase A antibody \| Lactate dehydrogenase B antibody \| Lactate dehydrogenase c var
规格或纯度	重组, ExactAb™, 已验证, 高性能, 见COA
宿主种属	兔(Rabbit)
特异性	Lactate Dehydrogenase
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	A synthetic peptide derived from human Lactate Dehydrogenase (AA 41-53).
阳性对照	WB: K562, HeLa, MCF7, HEK293, A549, NIH3T3 and C6 cell lysates. IHC: Human breast cancer tissue. ICC/IF: HeLa cells.
偶联	Unconjugated

产品属性

克隆类型	重组抗体
Format	Whole IgG
亚型	Rabbit IgG
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4
防腐剂	0.01% Sodium azide
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

CEND1 Tbio 细胞周期退出和神经元分化蛋白 1(Cell cycle exit and neuronal differentiation protein 1) (0 活性数据)

点击查看靶蛋白信息： CEND1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

LDHA Tchem L-乳酸脱氢酶A链(L-lactate dehydrogenase A chain) (0 活性数据)

点击查看靶蛋白信息： LDHA

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: Recombinant Lactate Dehydrogenase Antibody (Ab112519)

Recombinant Lactate Dehydrogenase Antibody (Ab112519) - Western Blot
All lanes: Recombinant Lactate Dehydrogenase Antibody (Ab112519) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 37 kDa
Observed band size: 35 kDa
Exposure time: 12.1 s

IF/ICC: Recombinant Lactate Dehydrogenase Antibody (Ab112519)

Recombinant Lactate Dehydrogenase Antibody (Ab112519) - IF/ICC
Immunocytochemistry analysis of Lactate Dehydrogenase (green) in HeLa using Recombinant Lactate Dehydrogenase Antibody (Ab112519), and cells were counterstained with DAPI (blue).

IHC: Recombinant Lactate Dehydrogenase Antibody (Ab112519)

Recombinant Lactate Dehydrogenase Antibody (Ab112519) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant Lactate Dehydrogenase Antibody (Ab112519). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

应用

应用名称	稀释比例
IF/ICC	1/50-1/200
IHC	1/100 - 1/200
WB	1/1000 - 1/5000

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到5个结果

批号(Lot Number)	证书类型	货号
ZJ25F0318891	分析证书	Ab112519
ZJ25F0318890	分析证书	Ab112519
ZJ25F0318889	分析证书	Ab112519
ZJ24F0202754	分析证书	Ab112519
ZJ24F0202753	分析证书	Ab112519

技术文档和文章

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化冷冻切片

免疫组织化学染色的抗原修复方案

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化抗原修复

免疫肽封闭实验

Categories: 实验方案（Protocols）

探索主题:

多克隆抗体免疫肽封闭特异性结合

Immunopeptide blocking assay

Categories: 实验方案（Protocols）

探索主题:

Immunopeptideblocking PolyclonalAntibody SpecificBinding

Immunopeptide blocking assay

Categories: 实验方案（Protocols）

探索主题:

Immunopeptideblocking PolyclonalAntibody SpecificBinding

免疫荧光染色实验步骤（甲醇通透法）

Categories: 实验方案（Protocols）

探索主题:

冷冻切片免疫荧光染色组织切片甲醇通透 IF

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: 实验方案（Protocols）

探索主题:

Frozen section IF Immunofluorescence staining Tissue section Methanol permeability

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: 实验方案（Protocols）

探索主题:

Frozen section IF Immunofluorescence staining Tissue section Methanol permeability

可替换产品

引用文献

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861]
2. Popovici-Muller J, Lemieux RM, Artin E, Saunders JO, Salituro FG, Travins J, Cianchetta G, Cai Z, Zhou D, Cui D et al.. (2018) Discovery of AG-120 (Ivosidenib): A First-in-Class Mutant IDH1 Inhibitor for the Treatment of IDH1 Mutant Cancers.. ACS Med Chem Lett, 9 (4): (300-305). [PMID:29670690]
3. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811]
4. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
5. Duveau DY, Yasgar A, Wang Y, Hu X, Kouznetsova J, Brimacombe KR, Jadhav A, Simeonov A, Thomas CJ, Maloney DJ.. (2014) Structure-activity relationship studies and biological characterization of human NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase inhibitors.. Bioorg Med Chem Lett, 24 (2): (630-635). [PMID:24360556] [10.1016/j.bmcl.2013.11.081]
6. Rai G, Brimacombe KR, Mott BT, Urban DJ, Hu X, Yang SM, Lee TD, Cheff DM, Kouznetsova J, Benavides GA, Pohida K, Kuenstner EJ, Luci DK, Lukacs CM, Davies DR, Dranow DM, Zhu H, Sulikowski G, Moore WJ, Stott GM, Flint AJ, Hall MD, Darley-Usmar VM, Neckers LM, Dang CV, Waterson AG, Simeonov A, Jadhav A, Maloney DJ.. (2017) Discovery and Optimization of Potent, Cell-Active Pyrazole-Based Inhibitors of Lactate Dehydrogenase (LDH).. J Med Chem, 60 (22): (9184-9204). [PMID:29120638] [10.1021/acs.jmedchem.7b00941]
7. Mullarky E, Xu J, Robin AD, Huggins DJ, Jennings A, Noguchi N, Olland A, Lakshminarasimhan D, Miller M, Tomita D, Michino M, Su T, Zhang G, Stamford AW, Meinke PT, Kargman S, Cantley LC.. (2019) Inhibition of 3-phosphoglycerate dehydrogenase (PHGDH) by indole amides abrogates de novo serine synthesis in cancer cells.. Bioorg Med Chem Lett, 29 (17): (2503-2510). [PMID:31327531] [10.1016/j.bmcl.2019.07.011]
8. Sudo, K K and 9 more authors.. (1992) Molecular analysis of genetic mutation in electrophoretic variant of human lactate dehydrogenase-A(M) subunit.. Biochemistry international, [PMID:1445373]
9. Maekawa, M M, Sudo, K K, Li, S S SS and Kanno, T T.. (1991) Analysis of genetic mutations in human lactate dehydrogenase-A(M) deficiency using DNA conformation polymorphism in combination with polyacrylamide gradient gel and silver staining.. Biochemical and biophysical research communications, (31): [PMID:1953713]
10. Maekawa, M M, Sudo, K K, Li, S S SS and Kanno, T T.. (1991) Genotypic analysis of families with lactate dehydrogenase A (M) deficiency by selective DNA amplification.. Human genetics, [PMID:1959923]
11. Maekawa, M M, Sudo, K K, Kanno, T T and Li, S S SS.. (1990) Molecular characterization of genetic mutation in human lactate dehydrogenase-A (M) deficiency.. Biochemical and biophysical research communications, (30): [PMID:2334430]
12. Chung, F Z FZ, Tsujibo, H H, Bhattacharyya, U U, Sharief, F S FS and LI, S S SS.. (1985) Genomic organization of human lactate dehydrogenase-A gene.. The Biochemical journal, (1): [PMID:3000353]
13. Tsujibo, H H, Tiano, H F HF and Li, S S SS.. (1985) Nucleotide sequences of the cDNA and an intronless pseudogene for human lactate dehydrogenase-A isozyme.. European journal of biochemistry, (15): [PMID:3838278]
14. Maekawa, M M and 5 more authors.. (1994) Fast-type electrophoretic variant of lactate dehydrogenase M(A) and comparison with other missense mutations in lactate dehydrogenase M(A) and H(B) genes.. Clinical chemistry, [PMID:7908613]
15. Scanlan, M J MJ and 11 more authors.. (1999) Antigens recognized by autologous antibody in patients with renal-cell carcinoma.. International journal of cancer, (12): [PMID:10508479]
16. Read, J A JA, Winter, V J VJ, Eszes, C M CM, Sessions, R B RB and Brady, R L RL.. (2001) Structural basis for altered activity of M- and H-isozyme forms of human lactate dehydrogenase.. Proteins, (1): [PMID:11276087]
17. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843]
18. Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, [PMID:15592455]
19. Giannakopoulos, Nadia V NV and 9 more authors.. (2005) Proteomic identification of proteins conjugated to ISG15 in mouse and human cells.. Biochemical and biophysical research communications, (21): [PMID:16139798]
20. O'Brien, Janet J, Kla, Koffi M KM, Hopkins, Irene B IB, Malecki, Elise A EA and McKenna, Mary C MC.. Kinetic parameters and lactate dehydrogenase isozyme activities support possible lactate utilization by neurons.. Neurochemical research, [PMID:17006762]
21. and Drăghia, Alina Cătălina AC.. (2006) Histochemical and histopathological study of the gastric mucosa in the portal hypertensive gastropathy.. Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, [PMID:17308685]
22. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
23. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895]
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26. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

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体积（添加到小瓶中）

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所需的复溶浓度

Recombinant Lactate Dehydrogenase Antibody

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器