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IL-18 Mouse mAb

    应用:
  • IHC
  • WB
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human)
  • 亚型: Mouse IgG1
  • 克隆号: C10
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab110010-10μl
10μl 现货 Stock Image
Ab110010-50μl
50μL 现货 Stock Image
Ab110010-100μl
100μL 现货 Stock Image
Ab110010-1ml
1ml 期货 Stock Image

基本信息

产品名称 IL-18 Mouse mAb
别名 白介素18(IL18)单克隆抗体 | IL-18 小鼠单克隆抗体 | IL-18 小鼠单抗
英文别名 Iboctadekin antibody | IFN gamma inducing factor antibody | IFN-gamma-inducing factor antibody | IGIF antibody | IL 1 gamma antibody | IL 18 antibody | IL 1g antibody | IL-1 gamma antibody | IL-18 antibody | IL1 gamma antibody | IL18 antibody | IL18 prote
规格或纯度 无载体, ExactAb™, 无叠氮钠, 已验证, 见COA
宿主种属 小鼠(Mouse)
特异性 IL18
种属反应性 人(Human)
免疫原 Recombinant IL18 expressed in E.coli (AA 94-193)
阳性对照 WB: HeLa, A431 and THP-1 cell lysates. IHC: Human kidney, spleen tissues.
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 C10
Format Whole IgG
亚型 Mouse IgG1
轻链亚型 kappa
SDS-PAGE 150 kDa
纯化方法 Protein A + Protein G purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂 0.05% Proclin 300
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

IL18 Tbio 白细胞介素-18(Interleukin-18) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

图片

IL-18 Mouse mAb (Ab110010) - Western Blot
All lanes: IL-18 Mouse mAb (Ab110010) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 20.7 s

IL-18 Mouse mAb (Ab110010) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL IL-18 Mouse mAb (Ab110010)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IL-18 Mouse mAb (Ab110010) - IHC
DAB staining on IHC
Sample: Human Spleen Tissue
Primary Ab: 20 µg/mL IL-18 Mouse mAb (Ab110010)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IL-18 Mouse mAb (Ab110010) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL IL-18 Mouse mAb (Ab110010)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IL-18 Mouse mAb (Ab110010) - IHC
DAB staining on IHC
Sample: Human Spleen Tissue
Primary Ab: 20 µg/mL IL-18 Mouse mAb (Ab110010)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

应用

应用名称 稀释比例
IHC

5-20 µg/mL

WB

0.5-2µg/mL

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ23F1101826 分析证书 Ab110010
ZJ23F1101829 分析证书 Ab110010
ZJ24F1011801 分析证书 Ab110010

引用文献

1. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki.  (2006-03-24)  Human chromosome 11 DNA sequence and analysis including novel gene identification..  Nature,  440  ((7083)): (497-500).  [PMID:16554811]
2. Ushio, S S and 15 more authors..  (1996)  Cloning of the cDNA for human IFN-gamma-inducing factor, expression in Escherichia coli, and studies on the biologic activities of the protein..  Journal of immunology (Baltimore, Md. : 1950),  (1): [PMID:8666798]
3. Tominaga, K K and 7 more authors..  (2000)  IL-12 synergizes with IL-18 or IL-1beta for IFN-gamma production from human T cells..  International immunology,  [PMID:10653850]
4. Kato, Zenichiro Z and 14 more authors..  (2003)  The structure and binding mode of interleukin-18..  Nature structural biology,  [PMID:14528293]
5. Gaggero, Alessia A and 7 more authors..  (2004)  A novel isoform of pro-interleukin-18 expressed in ovarian tumors is resistant to caspase-1 and -4 processing..  Oncogene,  (30): [PMID:15326478]
6. Jourdan, Tony T and 13 more authors..  (2013)  Activation of the Nlrp3 inflammasome in infiltrating macrophages by endocannabinoids mediates beta cell loss in type 2 diabetes..  Nature medicine,  [PMID:23955712]
7. Wei, Hui H and 6 more authors..  (2014)  Structural basis for the specific recognition of IL-18 by its alpha receptor..  FEBS letters,  (3): [PMID:25261253]
8. Tsutsumi, Naotaka N and 12 more authors..  (2014)  The structural basis for receptor recognition of human interleukin-18..  Nature communications,  (15): [PMID:25500532]
9. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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