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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab110010-10μl |
10μl |
现货 ![]() |
| |
| Ab110010-50μl |
50μL |
现货 ![]() |
| |
| Ab110010-100μl |
100μL |
现货 ![]() |
| |
| Ab110010-1ml |
1ml |
期货 ![]() |
|
| 产品名称 | IL-18 Mouse mAb |
|---|---|
| 别名 | 白介素18(IL18)单克隆抗体 | IL-18 小鼠单克隆抗体 | IL-18 小鼠单抗 |
| 英文别名 | Iboctadekin antibody | IFN gamma inducing factor antibody | IFN-gamma-inducing factor antibody | IGIF antibody | IL 1 gamma antibody | IL 18 antibody | IL 1g antibody | IL-1 gamma antibody | IL-18 antibody | IL1 gamma antibody | IL18 antibody | IL18 prote |
| 规格或纯度 | 无载体, ExactAb™, 无叠氮钠, 已验证, 见COA |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | IL18 |
| 种属反应性 | 人(Human) |
| 免疫原 | Recombinant IL18 expressed in E.coli (AA 94-193) |
| 阳性对照 | WB: HeLa, A431 and THP-1 cell lysates. IHC: Human kidney, spleen tissues. |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| 克隆号 | C10 |
| Format | Whole IgG |
| 亚型 | Mouse IgG1 |
| 轻链亚型 | kappa |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A + Protein G purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4 |
| 防腐剂 | 0.05% Proclin 300 |
| 浓度 | 见COA |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
IL-18 Mouse mAb (Ab110010) - Western Blot
All lanes: IL-18 Mouse mAb (Ab110010) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 20.7 s
IL-18 Mouse mAb (Ab110010) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL IL-18 Mouse mAb (Ab110010)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
IL-18 Mouse mAb (Ab110010) - IHC
DAB staining on IHC
Sample: Human Spleen Tissue
Primary Ab: 20 µg/mL IL-18 Mouse mAb (Ab110010)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
IL-18 Mouse mAb (Ab110010) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL IL-18 Mouse mAb (Ab110010)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
IL-18 Mouse mAb (Ab110010) - IHC
DAB staining on IHC
Sample: Human Spleen Tissue
Primary Ab: 20 µg/mL IL-18 Mouse mAb (Ab110010)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
| 应用名称 | 稀释比例 |
|---|---|
| IHC | 5-20 µg/mL |
| WB | 0.5-2µg/mL |
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab110010 | |
| 分析证书 | Ab110010 | |
| 分析证书 | Ab110010 |
¥499.90
| 1. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811] |
| 2. Ushio, S S and 15 more authors.. (1996) Cloning of the cDNA for human IFN-gamma-inducing factor, expression in Escherichia coli, and studies on the biologic activities of the protein.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:8666798] |
| 3. Tominaga, K K and 7 more authors.. (2000) IL-12 synergizes with IL-18 or IL-1beta for IFN-gamma production from human T cells.. International immunology, [PMID:10653850] |
| 4. Kato, Zenichiro Z and 14 more authors.. (2003) The structure and binding mode of interleukin-18.. Nature structural biology, [PMID:14528293] |
| 5. Gaggero, Alessia A and 7 more authors.. (2004) A novel isoform of pro-interleukin-18 expressed in ovarian tumors is resistant to caspase-1 and -4 processing.. Oncogene, (30): [PMID:15326478] |
| 6. Jourdan, Tony T and 13 more authors.. (2013) Activation of the Nlrp3 inflammasome in infiltrating macrophages by endocannabinoids mediates beta cell loss in type 2 diabetes.. Nature medicine, [PMID:23955712] |
| 7. Wei, Hui H and 6 more authors.. (2014) Structural basis for the specific recognition of IL-18 by its alpha receptor.. FEBS letters, (3): [PMID:25261253] |
| 8. Tsutsumi, Naotaka N and 12 more authors.. (2014) The structural basis for receptor recognition of human interleukin-18.. Nature communications, (15): [PMID:25500532] |
| 9. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |