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抗体
单克隆抗体
IDH1 Mouse mAb

IDH1 Mouse mAb

级别和纯度:

见COA

应用:

IHC
WB

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human), 小鼠(Mouse)
亚型: Mouse IgG2a
克隆号: C12
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货	价格	数量
Ab109324-10μl	10μl	现货
Ab109324-50μl	50μL	现货
Ab109324-100μl	100μL	期货
Ab109324-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: IDH1

Accession#:O75874 (1) Gene ID:3417 (2) IDH1 (2)

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基本信息

产品名称	IDH1 Mouse mAb
别名	异柠檬酸脱氢酶1单克隆抗体 \| 异柠檬酸脱氢酶1单克隆抗体 \| IDH1 小鼠单克隆抗体 \| IDH1 小鼠单抗
英文别名	Cytosolic NADP isocitrate dehydrogenase antibody \| Cytosolic NADP-isocitrate dehydrogenase antibody \| Epididymis luminal protein 216 antibody \| Epididymis secretory protein Li 26 antibody \| HEL-216 antibody \| HEL-S-26 antibody \| ICDH antibody \| IDCD antib
规格或纯度	无载体, ExactAb™, 已验证, 高性能, 见COA
宿主种属	小鼠(Mouse)
特异性	IDH1
种属反应性	人(Human),小鼠(Mouse)
免疫原	Recombinant IDH1 expressed in E.coli (AA 74-333).
阳性对照	WB: Hep G2 and NIH/3T3 cell lysates. IHC: Human colorectal cancer, stomach, kidney, prostate, glioma tissues.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	C12
Format	Whole IgG
亚型	Mouse IgG2a
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A + Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH 7.4.
防腐剂	0.02% Sodium azide
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

IDH1 Tclin 异柠檬酸脱氢酶 [NADP] 细胞质(Isocitrate dehydrogenase [NADP] cytoplasmic) (0 活性数据)

点击查看靶蛋白信息： IDH1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

IDH1 Tclin Isocitrate dehydrogenase [NADP] cytoplasmic (40980 活性数据)

点击查看靶蛋白信息： IDH1

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: IDH1 Mouse mAb (Ab109324)

IDH1 Mouse mAb (Ab109324) - Western Blot
All lanes: IDH1 Mouse mAb (Ab109324) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/40000 dilution

Predicted band size: 47 kDa
Observed band size: 44 kDa
Exposure time: 25.4 s

IHC: IDH1 Mouse mAb (Ab109324)

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Colorectal cancer Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IDH1 Mouse mAb (Ab109324)

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Stomach Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IDH1 Mouse mAb (Ab109324)

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IDH1 Mouse mAb (Ab109324)

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Prostate Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IDH1 Mouse mAb (Ab109324)

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Glioma Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IDH1 Mouse mAb (Ab109324)

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Prostate Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: IDH1 Mouse mAb (Ab109324)

IDH1 Mouse mAb (Ab109324) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

应用

应用名称	稀释比例
IHC	5-30 µg/mL
WB	0.01-2 µg/mL

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0303945	分析证书	Ab109324
ZJ24F0303944	分析证书	Ab109324
ZJ24F0303943	分析证书	Ab109324

技术文档和文章

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化冷冻切片

免疫组织化学染色的抗原修复方案

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化抗原修复

细胞内ELISA实验方案

Categories: 实验方案（Protocols）

探索主题:

单克隆抗体多克隆抗体细胞内酶联免疫吸附实验细胞印迹蛋白定量辣根过氧化物酶标记二抗

In-cell ELISA protocol

Categories: 实验方案（Protocols）

探索主题:

Monoclonal antibodies In-cell ELISA Cytoblot Protein quantification HRP-Conjugated Secondary Antibodies Polyclonal Antibodies

In-cell ELISA protocol

Categories: 实验方案（Protocols）

探索主题:

Monoclonal antibodies In-cell ELISA Cytoblot Protein quantification HRP-Conjugated Secondary Antibodies Polyclonal Antibodies

引用文献

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861]
2. Dang L, White DW, Gross S, Bennett BD, Bittinger MA, Driggers EM, Fantin VR, Jang HG, Jin S, Keenan MC et al.. (2009) Cancer-associated IDH1 mutations produce 2-hydroxyglutarate.. Nature, 462 (7274): (739-44). [PMID:19935646]
3. Okoye-Okafor UC, Bartholdy B, Cartier J, Gao EN, Pietrak B, Rendina AR, Rominger C, Quinn C, Smallwood A, Wiggall KJ et al.. (2015) New IDH1 mutant inhibitors for treatment of acute myeloid leukemia.. Nat Chem Biol, 11 (11): (878-86). [PMID:26436839]
4. Popovici-Muller J, Lemieux RM, Artin E, Saunders JO, Salituro FG, Travins J, Cianchetta G, Cai Z, Zhou D, Cui D et al.. (2018) Discovery of AG-120 (Ivosidenib): A First-in-Class Mutant IDH1 Inhibitor for the Treatment of IDH1 Mutant Cancers.. ACS Med Chem Lett, 9 (4): (300-305). [PMID:29670690]
5. Zhao Q, Manning JR, Sutton J, Costales A, Sendzik M, Shafer CM, Levell JR, Liu G, Caferro T, Cho YS et al.. (2018) Optimization of 3-Pyrimidin-4-yl-oxazolidin-2-ones as Orally Bioavailable and Brain Penetrant Mutant IDH1 Inhibitors.. ACS Med Chem Lett, 9 (7): (746-751). [PMID:30034612]
6. Caravella JA, Lin J, Diebold RB, Campbell AM, Ericsson A, Gustafson G, Wang Z, Castro J, Clarke A, Gotur D et al.. (2020) Structure-Based Design and Identification of FT-2102 (Olutasidenib), a Potent Mutant-Selective IDH1 Inhibitor.. J Med Chem, 63 (4): (1612-1623). [PMID:31971798]
7. Machida Y, Nakagawa M, Matsunaga H, Yamaguchi M, Ogawara Y, Shima Y, Yamagata K, Katsumoto T, Hattori A, Itoh M et al.. (2020) A Potent Blood-Brain Barrier-Permeable Mutant IDH1 Inhibitor Suppresses the Growth of Glioblastoma with IDH1 Mutation in a Patient-Derived Orthotopic Xenograft Model.. Mol Cancer Ther, 19 (2): (375-383). [PMID:31727689]
8. Zheng B, Yao Y, Liu Z, Deng L, Anglin JL, Jiang H, Prasad BV, Song Y.. (2013) Crystallographic Investigation and Selective Inhibition of Mutant Isocitrate Dehydrogenase.. ACS Med Chem Lett, 4 (6): (542-546). [PMID:23795241] [10.1021/ml400036z]
9. Liu Z, Yao Y, Kogiso M, Zheng B, Deng L, Qiu JJ, Dong S, Lv H, Gallo JM, Li XN, Song Y.. (2014) Inhibition of cancer-associated mutant isocitrate dehydrogenases: synthesis, structure-activity relationship, and selective antitumor activity.. J Med Chem, 57 (20): (8307-8318). [PMID:25271760] [10.1021/jm500660f]
10. Wu F, Jiang H, Zheng B, Kogiso M, Yao Y, Zhou C, Li XN, Song Y.. (2015) Inhibition of Cancer-Associated Mutant Isocitrate Dehydrogenases by 2-Thiohydantoin Compounds.. J Med Chem, 58 (17): (6899-6908). [PMID:26280302] [10.1021/acs.jmedchem.5b00684]
11. Rai G, Brimacombe KR, Mott BT, Urban DJ, Hu X, Yang SM, Lee TD, Cheff DM, Kouznetsova J, Benavides GA, Pohida K, Kuenstner EJ, Luci DK, Lukacs CM, Davies DR, Dranow DM, Zhu H, Sulikowski G, Moore WJ, Stott GM, Flint AJ, Hall MD, Darley-Usmar VM, Neckers LM, Dang CV, Waterson AG, Simeonov A, Jadhav A, Maloney DJ.. (2017) Discovery and Optimization of Potent, Cell-Active Pyrazole-Based Inhibitors of Lactate Dehydrogenase (LDH).. J Med Chem, 60 (22): (9184-9204). [PMID:29120638] [10.1021/acs.jmedchem.7b00941]
12. Cho YS, Levell JR, Liu G, Caferro T, Sutton J, Shafer CM, Costales A, Manning JR, Zhao Q, Sendzik M, Shultz M, Chenail G, Dooley J, Villalba B, Farsidjani A, Chen J, Kulathila R, Xie X, Dodd S, Gould T, Liang G, Heimbach T, Slocum K, Firestone B, Pu M, Pagliarini R, Growney JD.. (2017) Discovery and Evaluation of Clinical Candidate IDH305, a Brain Penetrant Mutant IDH1 Inhibitor.. ACS Med Chem Lett, 8 (10): (1116-1121). [PMID:29057061] [10.1021/acsmedchemlett.7b00342]
13. Ma T, Zou F, Pusch S, Yang L, Zhu Q, Xu Y, Gu Y, von Deimling A, Zha X.. (2017) Design, synthesis and biological activity of 3-pyrazine-2-yl-oxazolidin-2-ones as novel, potent and selective inhibitors of mutant isocitrate dehydrogenase 1.. Bioorg Med Chem, 25 (24): (6379-6387). [PMID:29089260] [10.1016/j.bmc.2017.10.009]
14. Zou F, Pusch S, Hua J, Ma T, Yang L, Zhu Q, Xu Y, Gu Y, von Deimling A, Zha X.. (2018) Identification of novel allosteric inhibitors of mutant isocitrate dehydrogenase 1 by cross docking-based virtual screening.. Bioorg Med Chem Lett, 28 (3): (388-393). [PMID:29290542] [10.1016/j.bmcl.2017.12.030]
15. Crane EA, Heydenreuter W, Beck KR, Strajhar P, Vomacka J, Smiesko M, Mons E, Barth L, Neuburger M, Vedani A, Odermatt A, Sieber SA, Gademann K.. (2019) Profiling withanolide A for therapeutic targets in neurodegenerative diseases.. Bioorg Med Chem, 27 (12): (2508-2520). [PMID:30929949] [10.1016/j.bmc.2019.03.022]
16. Mullarky E, Xu J, Robin AD, Huggins DJ, Jennings A, Noguchi N, Olland A, Lakshminarasimhan D, Miller M, Tomita D, Michino M, Su T, Zhang G, Stamford AW, Meinke PT, Kargman S, Cantley LC.. (2019) Inhibition of 3-phosphoglycerate dehydrogenase (PHGDH) by indole amides abrogates de novo serine synthesis in cancer cells.. Bioorg Med Chem Lett, 29 (17): (2503-2510). [PMID:31327531] [10.1016/j.bmcl.2019.07.011]
17. Lin J, Lu W, Caravella JA, Campbell AM, Diebold RB, Ericsson A, Fritzen E, Gustafson GR, Lancia DR, Shelekhin T, Wang Z, Castro J, Clarke A, Gotur D, Josephine HR, Katz M, Diep H, Kershaw M, Yao L, Kauffman G, Hubbs SE, Luke GP, Toms AV, Wang L, Bair KW, Barr KJ, Dinsmore C, Walker D, Ashwell S.. (2019) Discovery and Optimization of Quinolinone Derivatives as Potent, Selective, and Orally Bioavailable Mutant Isocitrate Dehydrogenase 1 (mIDH1) Inhibitors.. J Med Chem, 62 (14): (6575-6596). [PMID:31199148] [10.1021/acs.jmedchem.9b00362]
18. Duan Z, Liu J, Niu L, Wang J, Feng M, Chen H, Luo C.. (2019) Discovery of DC_H31 as potential mutant IDH1 inhibitor through NADPH-based high throughput screening.. Bioorg Med Chem, 27 (15): (3229-3236). [PMID:31208797] [10.1016/j.bmc.2019.05.040]
19. Cao H, Zhu G, Sun L, Chen G, Ma X, Luo X, Zhu J.. (2019) Discovery of new small molecule inhibitors targeting isocitrate dehydrogenase 1 (IDH1) with blood-brain barrier penetration.. Eur J Med Chem, 183 (111694-111694). [PMID:31561044] [10.1016/j.ejmech.2019.111694]
20. Hu C, Zuo Y, Liu J, Xu H, Liao W, Dang Y, Luo C, Tang L, Zhang H.. (2020) Licochalcone A suppresses the proliferation of sarcoma HT-1080 cells, as a selective R132C mutant IDH1 inhibitor.. Bioorg Med Chem Lett, 30 (2): (126825-126825). [PMID:31836442] [10.1016/j.bmcl.2019.126825]
21. Nekrutenko, A A, Hillis, D M DM, Patton, J C JC, Bradley, R D RD and Baker, R J RJ.. (1998) Cytosolic isocitrate dehydrogenase in humans, mice, and voles and phylogenetic analysis of the enzyme family.. Molecular biology and evolution, [PMID:9866202]
22. Geisbrecht, B V BV and Gould, S J SJ.. (1999) The human PICD gene encodes a cytoplasmic and peroxisomal NADP(+)-dependent isocitrate dehydrogenase.. The Journal of biological chemistry, (22): [PMID:10521434]
23. Wiemann, S S and 23 more authors.. (2001) Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.. Genome research, [PMID:11230166]
24. Xu, Xiang X and 6 more authors.. (2004) Structures of human cytosolic NADP-dependent isocitrate dehydrogenase reveal a novel self-regulatory mechanism of activity.. The Journal of biological chemistry, (6): [PMID:15173171]
25. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621]
26. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): [PMID:16959974]
27. Bechtel, Stephanie S and 18 more authors.. (2007) The full-ORF clone resource of the German cDNA Consortium.. BMC genomics, (31): [PMID:17974005]
28. Parsons, D Williams DW and 32 more authors.. (2008) An integrated genomic analysis of human glioblastoma multiforme.. Science (New York, N.Y.), (26): [PMID:18772396]
29. Bleeker, Fonnet E FE and 16 more authors.. (2009) IDH1 mutations at residue p.R132 (IDH1(R132)) occur frequently in high-grade gliomas but not in other solid tumors.. Human mutation, [PMID:19117336]
30. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378]
31. Davis, Mindy I MI and 14 more authors.. (2014) Biochemical, cellular, and biophysical characterization of a potent inhibitor of mutant isocitrate dehydrogenase IDH1.. The Journal of biological chemistry, (16): [PMID:24668804]
32. Jin, Yonghui Y and 13 more authors.. (2015) Mutant IDH1 Dysregulates the Differentiation of Mesenchymal Stem Cells in Association with Gene-Specific Histone Modifications to Cartilage- and Bone-Related Genes.. PloS one, [PMID:26161668]
33. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

溶液计算器

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计算溶液所需的质量、体积或浓度。

质量

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浓度

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g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

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体积 1 (V1)

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浓度 2 (C2)

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复溶计算器

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所需的复溶浓度

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