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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab129534-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$79.90
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Ab129534-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$229.90
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Ab129534-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$379.90
|
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Ab129534-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,999.90
|
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pAb; Rabbit anti Human STEAP2 Antibody; WB, IHC, IF/ICC; Unconjugated
| Product Name | STEAP2 Antibody - Primary antibody, specific to STEAP2, Rabbit IgG |
|---|---|
| Synonyms | STEAP2 | STMP | IPCA1 | PUMPCn | STAMP1 | PCANAP1 | 4921538B17Rik antibody | AI930049 antibody | IPCA 1 antibody | IPCA1 antibody | Metalloreductase STEAP2 antibody | PCANAP 1 antibody | PCANAP1 antibody | Prostate cancer associated protein 1 antibody | P |
| Specifications & Purity | Carrier Free, ExactAb™, Validated, 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor. |
| Host species | Rabbit |
| Specificity | STEAP2 |
| Immunogen | A KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human STEAP2. |
| Positive Control | WB: HEK293 and LNCaP cell lysates. IHC: Human colon cancer tissue. IF/ICC: HEK293T cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Rabbit anti Human STEAP2 Antibody, Polyclonal antibody, could be used for WB, IHC, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Recombinant HSF1 Antibody (Ab129534) - Western Blot
All lanes: Recombinant HSF1 Antibody (Ab129534) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 56 kDa
Observed band size: 47 kDa
Exposure time: 100.0 s
Recombinant HSF1 Antibody (Ab129534) - IHC
Immunohistochemical analysis of STEAP2 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Recombinant HSF1 Antibody (Ab129534) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Recombinant HSF1 Antibody (Ab129534) - IF/ICC
Immunofluorescent analysis of STEAP2 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Recombinant HSF1 Antibody (Ab129534) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
| Application | Dilution info |
|---|---|
| IHC | 1/50-1/200 |
| WB |
1/500-1/2000
|
| IF/ICC | 1/50-1/100 |