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Recombinant TFII I Antibody - Primary antibody, specific to GTF2I, Rabbit IgG

COA COA
    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab130795
Grouped product items
SKU Size
Availability
 Price Qty
Ab130795-10μl
10μl
1
$69.90
Ab130795-50μl
50μl
1
$189.90
Ab130795-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$289.90
Ab130795-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti TFII I Antibody; WB, IHC, ICC, IF; Unconjugated

View related series
Click to view target information: GTF2I
Accession#: P78347 (1) Gene ID: 2969 (1) General Transcription Factor II I (1) GTF2I (1) TFII I (1)

Basic Description

Product Name Recombinant TFII I Antibody - Primary antibody, specific to GTF2I, Rabbit IgG
Synonyms BAP 135 antibody | BAP-135 antibody | BAP135 antibody | Bruton tyrosine kinase associated protein 135 antibody | Bruton tyrosine kinase-associated protein 135 antibody | BTK associated protein 135 antibody | BTK associated protein 135kD antibody | BTK ass
Specifications & Purity ExactAb™, Validated, Recombinant, 0.2 mg/mL
Host species Rabbit
Specificity GTF2I
Immunogen A synthetic peptide derived from human TFII I (AA 66-80)
Positive Control WB: HEEK293, A549, HeLa, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human TFII I Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Antigen affinity purified
Shape Liquid
Concentration 0.2 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

GTF2I Tbio General transcription factor II-I (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant TFII I Antibody (Ab130795) - Western Blot
All lanes: Recombinant TFII I Antibody (Ab130795) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 112 kDa
Observed band size: 129 kDa
Exposure time: 14.7 s

Recombinant TFII I Antibody (Ab130795) - IF/ICC
Immunocytochemistry analysis of General Transcription Factor II I (green) in HeLa using Recombinant TFII I Antibody (Ab130795) and DAPI (blue).

Recombinant TFII I Antibody (Ab130795) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using Recombinant TFII I Antibody (Ab130795). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. 

Application

Application Dilution info
IF/ICC

1/50-1/200

IHC

1/50 - 1/100
WB

1/500-1/1000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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