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Recombinant SOX10 Antibody - Primary antibody, specific to SOX10, Rabbit IgG

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • IHC
  • IP
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Rat
  • Isotype: Rabbit IgG
  • Clone Number: RR643
  • Conjugation: Unconjugated
In stock
Item Number
Ab128734
Grouped product items
SKU Size
Availability
 Price Qty
Ab128734-10μl
10μl
1
$69.90
Ab128734-50μl
50μl
3
$207.90
Ab128734-100μl
100μl
3
$297.90
Ab128734-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,817.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti Human SOX10 Antibody; WB, IHC, IF/ICC, Flow Cytometry, IP; Unconjugated

View related series
Click to view target information: SOX10
Accession#:P56693 (1) Flow Cytometry (1158) Gene ID:6663 (1) SOX10 (1)

Basic Description

Product Name Recombinant SOX10 Antibody - Primary antibody, specific to SOX10, Rabbit IgG
Synonyms DOMantibody | DOM antibody | Dominant megacolon mouse human homolog of antibody | MGC15649 antibody | PCWH antibody | SOX 10 antibody | SOX10 antibody | SOX10_HUMAN antibody | SRY (sex determining region Y) box 10antibody | SRY (sex determining region Y)
Specifications & Purity Recombinant, ExactAb™, Validated, High Performance, See COA
Biochemical and Physiological Mechanisms Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia.
Host species Rabbit
Specificity SOX10
Immunogen A synthetic peptide derived from human SOX10 (AA 400-500).
Positive Control WB: A375 and C6 cell lysates. IHC: Human melanoma tissue. Flow: A375 cells. ICC/IF: A375 cells. IP: A375 whole cell lysate
Conjugation Unconjugated
Grade ExactAb™, High Performance, Recombinant, Validated
Product Description

Rabbit anti Human SOX10 Antibody, Recombinant, could be used for WB, IHC, IF/ICC, Flow Cytometry, IP and so on.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

SOX10 Tbio Transcription factor SOX-10 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant SOX10 Antibody (Ab128734) - Western Blot
All lanes: Recombinant SOX10 Antibody (Ab128734) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 49 kDa
Observed band size: 57, 70 kDa
Exposure time: 1.1 s 

Recombinant SOX10 Antibody (Ab128734) - IF/ICC
Recombinant SOX10 Antibody (Ab128734) staining SOX10 in A375 cells by IF/ICC. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant SOX10 Antibody (Ab128734) (1/10000) at 4°C. An AF488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An AF488-conjugated Goat Anti-Rabbit IgG (1/500).

Recombinant SOX10 Antibody ( Ab128734 ) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human melanoma tissue labelling SOX10 with Recombinant SOX10 Antibody (Ab128734) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Recombinant SOX10 Antibody (Ab128734) - IP
SOX10 was immunoprecipitated from 0.4 mg of A375 cell lysate with Recombinant SOX10 Antibody (Ab128734) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant SOX10 Antibody (Ab128734) IP in A375 whole cell lysate
Lane 2: Rabbit IgG instead of Recombinant SOX10 Antibody (Ab128734) in A375 whole cell lysate
Lane3: A375 whole cell lysate, 10 μg (input)
Exposure: 120.0 s.

Recombinant SOX10 Antibody (Ab128734) - Flow Cytometry
Overlay histogram showing A375 cells stained with Recombinant SOX10 Antibody (Ab128734)(blue). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant SOX10 Antibody (Ab128734) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (red) was used as a control.

Application

Application Dilution info
WB

1/1000 - 1/2000
IP

1/50

Flow Cytometry

1/200 - 1/1000

IF/ICC

1/2000 - 1/10000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1201988 Certificate of Analysis Dec 13, 2023 Ab128734
ZJ23F1201987 Certificate of Analysis Dec 13, 2023 Ab128734

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