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Recombinant S6K1 Antibody - Primary antibody, specific to RPS6KB1, Rabbit IgG

COA COA
    Application:
  • IF/ICC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab126314
Grouped product items
SKU Size
Availability
 Price Qty
Ab126314-10μl
10μl
5
$66.90
Ab126314-50μl
50μl
2
$199.90
Ab126314-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$319.90
Ab126314-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti Human S6K1 Antibody; WB, ICC, IF; Unconjugated

View related series
Click to view target information: RPS6KB1
Accession#:P23443 (2) Gene ID:6198 (2) RPS6KB1 (2) S6K1 (1)

Basic Description

Product Name Recombinant S6K1 Antibody - Primary antibody, specific to RPS6KB1, Rabbit IgG
Synonyms RPS6KB1 | STK14A | Ribosomal protein S6 kinase beta-1 | S6K-beta-1 | S6K1 | 70 kDa ribosomal protein S6 kinase 1 | P70S6K1 | p70-S6K 1 | Ribosomal protein S6 kinase I | Serine/threonine-protein kinase 14A | p70 ribosomal S6 kinase alpha | p70 S6 kinas | 7
Specifications & Purity ExactAb™, Validated, Recombinant, 0.2 mg/mL
Host species Rabbit
Specificity RPS6KB1
Immunogen A synthetic peptide derived from human S6K1 (AA 1-17).
Positive Control WB: Raw264.7, NIH/3T3, A549, A431, C6 and K562 cell lysates. ICC/IF: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human S6K1 Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Function
Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Immunogen affinity purified
Shape Liquid
Concentration 0.2 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

RPS6KB1 Tchem Ribosomal protein S6 kinase beta-1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
RPS6KB1 Tchem Ribosomal protein S6 kinase 1 (4456 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant S6K1 Antibody (Ab126314) - Western Blot
All lanes: Recombinant S6K1 Antibody (Ab126314) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 70 kDa
Observed band size: 63 kDa
Exposure time: 1.0 s

Recombinant S6K1 Antibody (Ab126314) - IF
Immunocytochemistry analysis of S6K1 (green) in HeLa cells using Recombinant S6K1 Antibody (Ab126314) and cells were counterstained with DAPI (blue).

Application

Application Dilution info
WB

1/500-1/1000
IF/ICC

1/50-1/200

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0901069 Certificate of Analysis Sep 18, 2023 Ab126314
ZJ23F0901068 Certificate of Analysis Sep 18, 2023 Ab126314

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