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Recombinant MGEA5/OGA Antibody - Primary antibody, specific to OGA, Rabbit IgG

Grade & Purity:

See COA

Application:

IF/ICC
WB

Host species: Rabbit
Reactive species: Human,Mouse
Isotype: Rabbit IgG
Conjugation: Unconjugated

In stock

Item Number

Ab115221

Grouped product items
SKU	Size	Availability	Price
Ab115221-10μl	10μl	2	$69.90
Ab115221-50μl	50μl	1	$199.90
Ab115221-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$329.90
Ab115221-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,699.90

Recombinant; Rabbit anti Human MGEA5/OGA Antibody; WB, IF/ICC; Unconjugated

View related series

Click to view target information: OGA

Accession#: O60502 (1) Gene ID: 10724 (1) MGEA5 (1) NCOAT (1) OGA (1)

Basic Description

Product Name	Recombinant MGEA5/OGA Antibody - Primary antibody, specific to OGA, Rabbit IgG
Synonyms	MEA5 \| MGEA5 \| NCOAT \| Beta-hexosaminidase antibody \| Beta-N-acetylglucosaminidase antibody \| Beta-N-acetylhexosaminidase antibody \| Bifunctional protein NCOAT antibody \| HEXC antibody \| OGA \| Hyaluronidase in meningioma antibody \| KIAA0679 antibody \| MEA
Specifications & Purity	Recombinant, ExactAb™, Validated, High Performance, See COA
Host species	Rabbit
Specificity	OGA
Immunogen	A synthetic peptide derived from human MGEA5 (AA 2-63)
Positive Control	WB: HeLa, HEK293, and Hep G2 cell lysates. IF/ICC: HeLa cells.
Conjugation	Unconjugated
Grade	ExactAb™, High Performance, Recombinant, Validated
Product Description	Rabbit anti Human MGEA5/OGA Antibody, Recombinant, could be used for WB, IF/ICC and so on. Application: WB: 1/1000-1/5000 IF/ICC: 1/50-1/200 Protein Function: Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714). Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

OGA Tchem Protein O-GlcNAcase (0 Activities)

Discover Target: OGA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Western Blot: Recombinant MGEA5/OGA Antibody (Ab115221)

Recombinant MGEA5/OGA Antibody (Ab115221) - Western Blot
All lanes: Recombinant MGEA5/OGA Antibody (Ab115221) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 103 kDa
Observed band size: 119 kDa
Exposure time: 1.0 s

IF/ICC: Recombinant MGEA5/OGA Antibody (Ab115221)

Recombinant MGEA5/OGA Antibody (Ab115221) - IF/ICC
Immunofluorescent analysis of HeLa, using Recombinant MGEA5/OGA Antibody (Ab115221) at 1/50 dilution.

IF/ICC: Recombinant MGEA5/OGA Antibody (Ab115221)

Recombinant MGEA5/OGA Antibody (Ab115221) - IF/ICC
Immunofluorescent analysis of HeLa, using Recombinant MGEA5/OGA Antibody (Ab115221) at 1/50 dilution.

Application

Application	Dilution info
IF/ICC	1/50 - 1/200
WB	1/1000 - 1/5000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0708423	Certificate of Analysis	Jul 17, 2024	Ab115221
ZJ24F0708422	Certificate of Analysis	Jul 17, 2024	Ab115221

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Recombinant MGEA5/OGA Antibody - Primary antibody, specific to OGA, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

Recombinant MGEA5/OGA Antibody - Primary antibody, specific to OGA, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews