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Recombinant hnRNP C1+C2/HNRNPC Antibody - Primary antibody, specific to HNRNPC, Rabbit IgG

    Application:
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab108417
Grouped product items
SKU Size
Availability
Price Qty
Ab108417-10μl
10μl
1
$69.90
Ab108417-50μl
50μl
1
$199.90
Ab108417-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab108417-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,199.90

Recombinant; Rabbit anti Human hnRNP C1+C2/HNRNPC Antibody; WB, IHC; Unconjugated

Basic Description

Product Name Recombinant hnRNP C1+C2/HNRNPC Antibody - Primary antibody, specific to HNRNPC, Rabbit IgG
Synonyms C2 antibody | Heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody | Heterogeneous nuclear ribonucleoprotein C antibody | Heterogeneous nuclear ribonucleoproteins C1/C2 antibody | HNRNP antibody | hnRNP C1 / hnRNP C2 antibody | hnRNP C1/C2 antibody
Specifications & Purity Recombinant, ExactAb™, Validated, High Performance, See COA
Host species Rabbit
Specificity HNRNPC
Immunogen A synthetic peptide derived from human hnRNP C1/C2 (AA 100-150)
Positive Control WB: HeLa, Hep G2, MOLT-4, K562, MCF7, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human colon tissue.
Conjugation Unconjugated
Grade ExactAb™, High Performance, Recombinant, Validated
Product Description

Rabbit anti Human hnRNP C1+C2/HNRNPC Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/5000-1/20000
IHC: 1/100-1/500
Protein Function
Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles (PubMed:8264621). Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs that affects mRNA splicing, processing and stability. M6A alters the local structure in mRNAs and long non-coding RNAs (lncRNAs) via a mechanism named 'm(6)A-switch' to facilitate binding of HNRNPC, leading to regulation of mRNA splicing (PubMed:25719671). Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules (PubMed:12509468, PubMed:16010978, PubMed:7567451, PubMed:8264621).
Post-translational
Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide. Sumoylated. Sumoylation reduces affinity for mRNA.

Product Properties

Isotype Rabbit IgG
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

HNRNPC Tbio Heterogeneous nuclear ribonucleoproteins C1/C2 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant hnRNP C1+C2/HNRNPC Antibody (Ab108417) - Western Blot
All lanes: Recombinant hnRNP C1+C2/HNRNPC Antibody (Ab108417) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 40 kDa
Exposure time: 7.8 s

Recombinant hnRNP C1+C2/HNRNPC Antibody (Ab108417) - IHC
Immunohistochemical analysis of paraffin-embedded human colon, using Recombinant hnRNP C1+C2/HNRNPC Antibody (Ab108417).

Application

Application Dilution info
IHC

1/100-1/500

WB

1/5000 - 1/20000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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