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Recombinant Fibrillarin Antibody - Primary antibody, specific to Fibrillarin, Rabbit IgG

COA COA
    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab103454
Grouped product items
SKU Size
Availability
 Price Qty
Ab103454-10μl
10μl
1
$69.90
Ab103454-50μl
50μl
1
$199.90
Ab103454-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab103454-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,299.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; Rabbit anti Human Fibrillarin Antibody; WB, IHC, ICC, IF; Unconjugated

View related series
Click to view target information: FBL
Accession#: P22087 (1) FBL (1) Fibrillarin (1) Gene ID: 2091 (1)

Basic Description

Product Name Recombinant Fibrillarin Antibody - Primary antibody, specific to Fibrillarin, Rabbit IgG
Synonyms FLRN | Nop1 | RNU3IP1 | 34 kD nucleolar scleroderma antigen antibody | 34 kDa nucleolar scleroderma antigen antibody | Fbl antibody | FBRL_HUMAN antibody | FIB antibody | FIB | FIB1 antibody | FLRN antibody | Histone-glutamine methyltransferase antibody |
Specifications & Purity Recombinant, ExactAb™, Validated, See COA
Host species Rabbit
Specificity Fibrillarin
Immunogen A synthesized peptide derived from human Fibrillarin (AA 270-321)
Positive Control WB: Hep G2, HEK293, U-2 OS, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human testis tissue. ICC/IF: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Fibrillarin Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/2000
IHC: 1/50-1/100
ICC/IF: 1/50-1/100
Protein Function：
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).

Product Properties

Isotype Rabbit IgG
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

FBL Tbio rRNA 2'-O-methyltransferase fibrillarin (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant Fibrillarin Antibody (Ab103454) - Western Blot
All lanes: Recombinant Fibrillarin Antibody (Ab103454) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 35 kDa
Exposure time: 2.1 s

Recombinant Fibrillarin Antibody (Ab103454) - Western Blot
All lanes: Recombinant Fibrillarin Antibody (Ab103454) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 35 kDa
Exposure time: 2.1 s

Recombinant Fibrillarin Antibody (Ab103454) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant Fibrillarin Antibody (Ab103454).

Recombinant Fibrillarin Antibody (Ab103454) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant Fibrillarin Antibody (Ab103454) at 1/50 dilution.

Recombinant Fibrillarin Antibody (Ab103454) - IHC
Immunohistochemical analysis of paraffin-embedded human testis, using Recombinant Fibrillarin Antibody (Ab103454).

Application

Application Dilution info
IHC

1/50 - 1/100
IF/ICC

1/50-1/100
WB

1/500-1/2000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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