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Recombinant EGFR Antibody (Biotin) - Primary antibody, specific to EGFR, Human IgG2SA

COA COA
    Application:
  • Flow Cytometry
  • Host species: Human
  • Reactive species: Human
  • Isotype: Human IgG2SA
  • Conjugation: Biotin
In stock
Item Number
Ab176025
Grouped product items
SKU Size
Availability
 Price Qty
Ab176025-10μg
10μg
≥10
$69.90
Ab176025-100μg
100μg
2
$299.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant; EGFR Antibody (Biotin); Flow

View related series
Click to view target information: EGFR
Accession#:P00533 (37) EGFR (254) Flow Cytometry (1158) Gene ID:1956 (38)

Basic Description

Product Name Recombinant EGFR Antibody (Biotin) - Primary antibody, specific to EGFR, Human IgG2SA
Synonyms EC 2.7.10.1 | Proto-oncogene c-ErbB-1 | Receptor tyrosine-protein kinase erbB-1 | Cell growth inhibiting protein 40 antibody | Cell proliferation inducing protein 61 antibody | EGF R antibody | EGFR antibody | EGFR_HUMAN antibody | Epidermal growth factor
Specifications & Purity ExactAb™, Recombinant, Validated, Azide Free, 1.0 mg/mL
Host species Human
Specificity EGFR
Positive Control Flow: A431
Conjugation Biotin
Grade Azide Free, ExactAb™, Recombinant, Validated
Product Description

Application:
Flow: ≤1.0μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1.0μg per million cells in 100 µL staining volume.

Product Properties

Isotype Human IgG2SA
Light Chain Type kappa
Purification Method Protein A purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

EGFR Tclin Epidermal growth factor receptor (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
EGFR Tclin Epidermal growth factor receptor erbB1 (33727 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
EGFR Tclin MER intracellular domain/EGFR extracellular domain chimera (2 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
EGFR Tclin Protein cereblon/Epidermal growth factor receptor (191 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
EGFR Tclin EGFR/PPP1CA (1 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
EGFR Tclin VHL/EGFR (323 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant EGFR Antibody (Biotin) (Ab176025) - Flow Cytometry
Flow cytometry analysis of EGFR expression by A431 cells (red) compared to an isotype control (blue). Cells were stained with Recombinant EGFR Antibody (Biotin) (Ab176025) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤1.0μg/ test

For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0102568 Certificate of Analysis Jan 24, 2024 Ab176025
ZJ24F0102567 Certificate of Analysis Jan 24, 2024 Ab176025
ZJ24R0100228 Certificate of Analysis Jan 19, 2024 Ab176025

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