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EGFR Mouse mAb (AF555) - Primary antibody, specific to EGFR

    Application:
  • Flow Cytometry
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG2b
  • Clone Number: EGFR.1
  • Conjugation: AF555
In stock
Item Number
Ab217689
Grouped product items
SKU Size
Availability
Price Qty
Ab217689-10T
10T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$61.90
Ab217689-25T
25T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$121.90
Ab217689-100T
100T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$329.90

mAb (EGFR.1); Mouse anti Human EGFR Antibody (AF555); Flow Cytometry

Basic Description

Product Name EGFR Mouse mAb (AF555) - Primary antibody, specific to EGFR
Synonyms Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody | Cell growth inhibiting protein 40 antibody | Cell proliferation inducing protein 61 antibody | EGF R antibody | EGFR antibody | EGFR_HUMAN antibody | Epidermal growth factor recepto
Specifications & Purity ExactAb™, Validated, 5 μL/test
Biochemical and Physiological Mechanisms Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin,
Host species Mouse
Specificity EGFR
Immunogen A431 cultured cells
Positive Control Flow Cytometry: A431
Conjugation AF555
Excitation(nm) Green Laser(532nm)
Emission(nm) 568nm
Ex/Em(nm) Ex:553nm;Em:568nm
Grade ExactAb™, Validated
Product Description

Mouse anti Human EGFR Antibody, Monoclonal (EGFR.1), could be used for Flow Cytometry.

Product Properties

Light Chain Type kappa
Purification Method Protein G purified
Concentration 5 μL/test
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

EGFR Tclin Epidermal growth factor receptor (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

EGFR Mouse mAb (AF555) (Ab217689) - Flow Cytometry
Flow cytometry analysis of EGFR expression by A431 cells (red) compared to an isotype control (blue). Cells were stained with EGFR Mouse mAb (AF555) (Ab217689) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF555) (Ab190246). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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