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Goat Anti-Human IgG Fc (Cy5) - Secondary antibody

COA

Grade & Purity:

Validated

ExactAb™

1.0 mg/mL

Application:

Flow Cytometry
IF/ICC

Host species: Goat
Reactive species: Human
Isotype: Goat IgG
Conjugation: Cy5

In stock

Item Number

Ab216761

Grouped product items
SKU	Size	Availability	Price
Ab216761-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$10.90
Ab216761-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$29.90
Ab216761-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$99.90

pAb; Goat Anti-Human IgG Fc Antibody (Cy5); IF, Flow Cytometry

View related series

Cy5 (11) Goat Anti-Human IgG Fc Antibody (12)

Basic Description

Product Name	Goat Anti-Human IgG Fc (Cy5) - Secondary antibody
Synonyms	Goat Anti-Human IgG Fc Antibody \| Goat Anti Human IgG Fc Antibody \| Anti-Human IgG Fc Antibody \| Anti Human IgG Fc Antibody
Specifications & Purity	ExactAb™, Validated, 1.0 mg/mL
Host species	Goat
Immunogen	Human IgG Fc
Conjugation	Cy5
Excitation(nm)	Red Laser(633nm)
Emission(nm)	665nm
Ex/Em(nm)	Ex:647nm;Em:665nm
Grade	ExactAb™, Validated
Product Description	Goat Anti-Human IgG Fc Antibody, Secondary antibody, could be used for Flow Cytometry, IF and so on.

Product Properties

Purification Method	Antigen affinity chromatography
Concentration	1.0 mg/mL
Storage Temp	Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

IF: Goat Anti-Human IgG Fc (Cy5) (Ab216761)

Goat Anti-Human IgG Fc (Cy5) (Ab216761) - IF
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG Fc (Cy5) (Ab216761) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Human IgG Fc (Cy5) (Ab216761)

Goat Anti-Human IgG Fc (Cy5) (Ab216761) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD20 (red) with Ibritumomab (anti-CD20) (Ab191926). Goat Anti-Human IgG Fc (Cy5) (Ab216761) at a dilution of 1/900 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow Cytometry	1/100-1/1000
IF/ICC	1/100-1/1000

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