Determine the necessary mass, volume, or concentration for preparing a solution.
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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab137858-100μg
|
100μg |
10
|
$29.90
|
|
|
Ab137858-1mg
|
1mg |
4
|
$59.90
|
|
|
Ab137858-5mg
|
5mg |
1
|
$199.90
|
|
|
Ab137858-10mg
|
10mg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$339.90
|
|
Secondary Antibody; Goat Anti-Human IgG Fc Antibody; WB, CLIA, LF, GICA, EIA, ELISA; Unconjugated
| Product Name | Goat Anti-Human IgG Fc Antibody - Secondary antibody, Goat IgG |
|---|---|
| Synonyms | Goat Anti-Human IgG Fc Antibody | Goat Anti Human IgG Fc Antibody | Anti-Human IgG Fc Antibody | Anti Human IgG Fc Antibody |
| Specifications & Purity | Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA |
| Host species | Goat |
| Immunogen | Human IgG Fc |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Goat Anti-Human IgG Fc Antibody, Secondary antibody, could be used for WB, CLIA, LF, GICA, EIA, ELISA and so on. |
| Isotype | Goat IgG |
|---|---|
| SDS-PAGE | 150kDa |
| Purification Method | Antigen affinity chromatography |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
Goat Anti-Human IgG Fc Antibody (Ab137858) - ELISA
Goat Anti-Human IgG Fc Antibody (Ab137858) was tested by ELISA. The plate was coated with 0.5 μg/mL Human IgG (Ab170213). Goat Anti-Human IgG Fc Antibody was biotinylated then added at a series of different dilution ratios. Streptavidin-HRP (np156148) was used at 1/40000 dilution for this ELISA.
Goat Anti-Human IgG Fc Antibody (Ab137858) - ELISA
Cross-reactivity of Goat Anti-Human IgG Fc Antibody (Ab137858) was tested by ELISA. The wells were coated with the indicated IgG standards at 0.5 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Goat Anti-Human IgG Fc Antibody was biotinylated then added at different dilution ratio followed by incubation for 1h. Streptavidin-HRP (np156148) was used at 1/40000 dilution for this ELISA.
For the batch tested, Goat Anti-Human IgG Fc Antibody (Ab137858) showed a high cross-reactivity with Rabbit IgG and a low cross-reactivity with Mouse IgG.
| Application | Dilution info |
|---|---|
| CLIA | 0.5-10 µg/mL |
| EIA | 1/1000 - 1/50000 |
| WB | 1/500-1/10000 |
| LF | 0.5mg/mL-2.0mg/mL |
| GICA | 0.5mg/mL-2.0mg/mL |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | May 11, 2024 | Ab137858 | |
| Certificate of Analysis | May 11, 2024 | Ab137858 | |
| Certificate of Analysis | May 11, 2024 | Ab137858 |
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