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EGFR Mouse mAb - Primary antibody, specific to EGFR, Mouse IgG2b

COA COA
    Application:
  • Flow Cytometry
  • Functional Assay
  • IF/ICC
  • IHC
  • IP
  • IP-MS
  • Neutralization
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG2b
  • Clone Number: EGFR.1
  • Conjugation: Unconjugated
In stock
Item Number
Ab101156
Grouped product items
SKU Size
Availability
 Price Qty
Ab101156-10μg
10μg
9
$49.90
Ab101156-50μg
50μg
4
$149.90
Ab101156-100μg
100μg
2
$229.90
Ab101156-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$599.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (EGFR.1); Mouse anti Human EGFR Antibody; WB, IHC, ICC, IF, IP, Flow, Neutralization, IP-Mass Spectrometry; Unconjugated

View related series
Click to view target information: EGFR
Accession#:P00533 (37) EGFR (254) Flow Cytometry (1158) Gene ID:1956 (38)

Basic Description

Product Name EGFR Mouse mAb - Primary antibody, specific to EGFR, Mouse IgG2b
Synonyms Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody | Cell growth inhibiting protein 40 antibody | Cell proliferation inducing protein 61 antibody | EGF R antibody | EGFR antibody | EGFR_HUMAN antibody | Epidermal growth factor recepto
Specifications & Purity Carrier Free, Low Endotoxin, Azide Free, Validated, ≥95%(SDS-PAGE&HPLC), See COA
Host species Mouse
Specificity EGFR
Immunogen A431 cultured cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, Low Endotoxin, Validated
Product Description

Mouse anti Human EGFR Antibody, Monoclonal (EGFR.1), could be used for WB, IHC, ICC/IF, IP, Flow, Neutralization, IP-Mass Spectrometry and so on.
Application
ICC/IF: 5 µg/mL
IP: 2 µg/mL
Flow: 1-5 ug/mL
IHC: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
Neutralization: Use at an assay dependent concentration.
IP-Mass Spectrometry: Use at an assay dependent concentration.
Protein Function
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.

Product Properties

Isotype Mouse IgG2b
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

EGFR Tclin Epidermal growth factor receptor (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
EGFR Tclin Epidermal growth factor receptor erbB1 (33727 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
EGFR Tclin MER intracellular domain/EGFR extracellular domain chimera (2 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
EGFR Tclin Protein cereblon/Epidermal growth factor receptor (191 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
EGFR Tclin EGFR/PPP1CA (1 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
EGFR Tclin VHL/EGFR (323 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

EGFR Mouse mAb (Ab101156) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling EGFR (red) with EGFR Mouse mAb (Ab101156) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
IF/ICC

5.0 μg/mL
WB

Use at an assay dependent concentration.
Neutralization

Use at an assay dependent concentration.
IP

2 µg/mL
Flow

1-5 μg/mL
IHC

Use at an assay dependent concentration.
IP-MS

Use at an assay dependent concentration.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0707959 Certificate of Analysis Jul 09, 2024 Ab101156
ZJ24F0707958 Certificate of Analysis Jul 09, 2024 Ab101156
ZJ24F0707957 Certificate of Analysis Jul 09, 2024 Ab101156

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