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Goat Anti-Human IgG (Cy5) - Secondary antibody

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • Host species: Goat
  • Reactive species: Human
  • Conjugation: Cy5
In stock
Item Number
Ab175833
Grouped product items
SKU Size
Availability
 Price Qty
Ab175833-100μg
100μg
5
$49.90
Ab175833-1mg
1mg
1
$299.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Secondary Antibody; Goat Anti-Human IgG (Cy5); IF, Flow

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Basic Description

Product Name Goat Anti-Human IgG (Cy5) - Secondary antibody
Synonyms Goat Anti-Human Antibody (Cy5) | Cy5-conjugated Goat Anti-Human Antibody | Goat Anti-Human IgG / Cy5 antibody | Goat Anti-Human IgG-Cy5 antibody
Specifications & Purity ExactAb™, Validated, Azide Free, Ex:647nm, Em:665nm, 1.0 mg/mL
Host species Goat
Conjugation Cy5
Excitation(nm) Red Laser(633nm)
Emission(nm) 665nm
Ex/Em(nm) Ex:647nm;Em:665nm
Grade Azide Free, Em:665nm, Ex:647nm, ExactAb™, Validated
Product Description

Application
Flow: 1:100-1:1000
IF: 1:1000

Product Properties

Purification Method Antigen affinity purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

Goat Anti-Human IgG (Cy5) (Ab175833) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD20 (red) with Rituximab (anti-CD20) (R412001). Goat Anti-Human IgG (Cy5) (Ab175833) at a dilution of 1/100 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Goat Anti-Human IgG (Cy5) (Ab175833) - IF
IF analysis of EGFR (red) in A549 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Necitumumab (anti-EGFR) (Ab169264) at 2 μg/mL in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG (Cy5) (Ab175833) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

1:1000
Flow Cytometry

1/100-1/1000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1001385 Certificate of Analysis Jul 31, 2024 Ab175833
ZJ23F1001409 Certificate of Analysis Jul 31, 2024 Ab175833

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