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EGFR Mouse mAb (PE) - Primary antibody, specific to EGFR, Mouse IgG2b

COA COA
    Application:
  • Flow Cytometry
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG2b
  • Clone Number: EGFR.1
  • Conjugation: PE
In stock
Item Number
Ab190494
Grouped product items
SKU Size
Availability
 Price Qty
Ab190494-10T
10T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$54.90
Ab190494-25T
25T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$109.90
Ab190494-100T
100T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$306.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (EGFR.1); Mouse anti Human EGFR Antibody (PE); Flow

View related series
Click to view target information: EGFR
Accession#:P00533 (37) EGFR (254) ERBB (15) ERBB1 (19) ERRP (14) Flow Cytometry (1158) Gene ID:1956 (38) HER1 (19) mENA (14) NISBD2 (14) PE (167) PIG61 (14)

Basic Description

Product Name EGFR Mouse mAb (PE) - Primary antibody, specific to EGFR, Mouse IgG2b
Synonyms Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody | Cell growth inhibiting protein 40 antibody | Cell proliferation inducing protein 61 antibody | EGF R antibody | EGFR antibody | EGFR_HUMAN antibody | Epidermal growth factor recepto
Specifications & Purity ExactAb™, Validated, Ex:565nm, Em:575nm, 5 μL/test
Biochemical and Physiological Mechanisms Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin,
Host species Mouse
Specificity EGFR
Immunogen A431 cultured cells
Positive Control Flow: A431
Conjugation PE
Excitation(nm) Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm) 575nm
Ex/Em(nm) Ex:565nm;Em:575nm
Grade Em:575nm, Ex:565nm, ExactAb™, Validated
Product Description

Mouse anti Human EGFR Antibody, Monoclonal (EGFR.1), could be used for WB, IHC, ICC/IF, IP, Flow, Neutralization, IP-Mass Spectrometry and so on.

Product Properties

Isotype Mouse IgG2b
Light Chain Type kappa
Purification Method Protein G purified
Concentration 5 μL/test
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

EGFR Tclin Epidermal growth factor receptor (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
EGFR Tclin Epidermal growth factor receptor erbB1 (33727 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
EGFR Tclin MER intracellular domain/EGFR extracellular domain chimera (2 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
EGFR Tclin Protein cereblon/Epidermal growth factor receptor (191 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
EGFR Tclin EGFR/PPP1CA (1 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
EGFR Tclin VHL/EGFR (323 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

EGFR Mouse mAb (PE) (Ab190494) - Flow Cytometry
Flow cytometry analysis of EGFR expression by A431 cells (red) compared to an isotype control (blue). Cells were stained with EGFR Mouse mAb (PE) (Ab190494) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (PE) (Ab180308). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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