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Goat Anti-Human IgG (Biotin) - Secondary antibody

COA COA
    Application:
  • ELISA
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Goat
  • Reactive species: Human
  • Conjugation: Biotin
In stock
Item Number
Ab175832
Grouped product items
SKU Size
Availability
 Price Qty
Ab175832-100μg
100μg
5
$19.90
Ab175832-1mg
1mg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$119.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Secondary Antibody; Goat Anti-Human IgG (Biotin); ELISA, WB, IF, Flow

View related series
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Basic Description

Product Name Goat Anti-Human IgG (Biotin) - Secondary antibody
Synonyms Goat Anti-Human Antibody (Biotin) | Biotin-conjugated Goat Anti-Human Antibody | Goat Anti-Human IgG / Biotin antibody | Goat Anti-Human IgG-Biotin antibody
Specifications & Purity ExactAb™, High Performance, Validated, Azide Free, 1.0 mg/mL
Host species Goat
Conjugation Biotin
Grade Azide Free, ExactAb™, High Performance, Validated
Product Description

Application
ELISA: 1:10000-1:200000
WB: 1:10000-1:200000
Flow: 1:200-1:1000
IF: 1:1000

Product Properties

Purification Method Antigen affinity purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing
Shipped In Wet ice
Stability And Storage Store at 4°C for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

Goat Anti-Human IgG (Biotin) (Ab175832) - ELISA
Goat Anti-Human IgG (Biotin) (Ab175832) was tested by direct ELISA. The plate was coated with different amounts of Human IgG (Ab170213). A serial dilution of Goat Anti-Human IgG (Biotin) (Ab175832) was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.

Goat Anti-Human IgG (Biotin) (Ab175832) - ELISA
Cross-reactivity of Goat Anti-Human IgG (Biotin) (Ab175832) was tested by direct ELISA. The wells were coated with the indicated IgG at 0.5 µg/mL (100 µL/well) and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h. Goat Anti-Human IgG (Biotin) (Ab175832) at 1/10000 dilution was used as the secondary antibody. A HRP-streptavidin (np156148) was used at 1/20000 dilution for this ELISA.

Application

Application Dilution info
WB

1/10000-1/200000
IF/ICC

1:1000
Flow Cytometry

1/200 - 1/1000

ELISA

1:10000-1:200000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24R1201654 Certificate of Analysis Dec 26, 2024 Ab175832
ZJ23F1001381 Certificate of Analysis Oct 26, 2023 Ab175832
ZJ23F1001382 Certificate of Analysis Oct 26, 2023 Ab175832

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