Determine the necessary mass, volume, or concentration for preparing a solution.
This is a demo store. No orders will be fulfilled.
| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab100782-10μl
|
10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$59.90
|
|
|
Ab100782-50μl
|
50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$179.90
|
|
|
Ab100782-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$289.90
|
|
|
Ab100782-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,699.90
|
|
Recombinant; Rabbit anti Human E2F1 Antibody; WB, IHC, IF/ICC; Unconjugated
| Product Name | Recombinant E2F1 Antibody - Primary antibody, specific to E2F1, Rabbit IgG |
|---|---|
| Synonyms | Dmel\CG6376 antibody | Dmel_CG6376 antibody | drosE2F1 antibody | E(Sev-CycE)3A antibody | E(var)3-93E antibody | E2-promoter binding facto antibody | E2F 1 antibody | E2F transcription factor 1 antibody | E2F-1 antibody | E2f-PA antibody | E2f-PB antibod |
| Specifications & Purity | Recombinant, ExactAb™, KD Validation, Validated, See COA |
| Biochemical and Physiological Mechanisms | Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF |
| Host species | Rabbit |
| Specificity | E2F1 |
| Immunogen | A synthetic peptide derived from human E2F1 (AA 365-400) |
| Positive Control | WB: HeLa, A375, NIH/3T3 and C6 cell lysates. IHC: Human ovary carcinoma tissue. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, KD Validation, Recombinant, Validated |
| Product Description |
Rabbit anti Human E2F1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Recombinant E2F1 Antibody (Ab100782) - Western Blot
All lanes: Recombinant E2F1 Antibody (Ab100782) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 66, 74 kDa
Exposure time: 3.8 s
Recombinant E2F1 Antibody (Ab100782) - Western Blot
All lanes use Recombinant E2F1 Antibody (Ab100782) at 1/1000 dilution for 1 hour at room temperature.
Recombinant E2F1 Antibody (Ab100782) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782) at 1/50 dilution.
Recombinant E2F1 Antibody (Ab100782) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782) at 1/50 dilution.
Recombinant E2F1 Antibody (Ab100782) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782).
Recombinant E2F1 Antibody (Ab100782) - IHC
Immunohistochemical analysis of paraffin-embedded human ovary carcinoma, using Recombinant E2F1 Antibody (Ab100782).
| Application | Dilution info |
|---|---|
| IHC | 1/100 - 1/200 |
| WB | 1/1000 - 1/2000 |
| IF/ICC | 1/50 - 1/200 |