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Recombinant E2F1 Antibody - Primary antibody, specific to E2F1, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab100782
Grouped product items
SKU Size
Availability
Price Qty
Ab100782-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
Ab100782-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$179.90
Ab100782-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$289.90
Ab100782-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human E2F1 Antibody; WB, IHC, IF/ICC; Unconjugated

Basic Description

Product Name Recombinant E2F1 Antibody - Primary antibody, specific to E2F1, Rabbit IgG
Synonyms Dmel\CG6376 antibody | Dmel_CG6376 antibody | drosE2F1 antibody | E(Sev-CycE)3A antibody | E(var)3-93E antibody | E2-promoter binding facto antibody | E2F 1 antibody | E2F transcription factor 1 antibody | E2F-1 antibody | E2f-PA antibody | E2f-PB antibod
Specifications & Purity Recombinant, ExactAb™, KD Validation, Validated, See COA
Biochemical and Physiological Mechanisms Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF
Host species Rabbit
Specificity E2F1
Immunogen A synthetic peptide derived from human E2F1 (AA 365-400)
Positive Control WB: HeLa, A375, NIH/3T3 and C6 cell lysates. IHC: Human ovary carcinoma tissue. IF/ICC: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, KD Validation, Recombinant, Validated
Product Description

Rabbit anti Human E2F1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.

Product Properties

Isotype Rabbit IgG
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

E2F1 Tbio Transcription factor E2F1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
E2F1 Tbio Transcription factor E2F1 (14 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant E2F1 Antibody (Ab100782) - Western Blot
All lanes: Recombinant E2F1 Antibody (Ab100782) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 66, 74 kDa
Exposure time: 3.8 s

Recombinant E2F1 Antibody (Ab100782) - Western Blot
All lanes use Recombinant E2F1 Antibody (Ab100782) at 1/1000 dilution for 1 hour at room temperature.

Recombinant E2F1 Antibody (Ab100782) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782) at 1/50 dilution.

Recombinant E2F1 Antibody (Ab100782) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782) at 1/50 dilution.

Recombinant E2F1 Antibody (Ab100782) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782).

Recombinant E2F1 Antibody (Ab100782) - IHC
Immunohistochemical analysis of paraffin-embedded human ovary carcinoma, using Recombinant E2F1 Antibody (Ab100782).

Application

Application Dilution info
IHC

1/100 - 1/200

WB

1/1000 - 1/2000

IF/ICC

1/50 - 1/200

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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