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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab099867-10μl
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10μl |
2
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$59.90
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Ab099867-50μl
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50μl |
1
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$189.90
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Ab099867-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$309.90
|
|
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Ab099867-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,399.90
|
|
Recombinant; Rabbit anti Human Dihydrofolate reductase/DHFR Antibody; WB, IF/ICC; Unconjugated
| Product Name | Recombinant Dihydrofolate reductase/DHFR Antibody - Primary antibody, specific to Dihydrofolate reductase, Rabbit IgG |
|---|---|
| Synonyms | DHFR antibody | DHFRP1 antibody | Dihydrofolate reductase antibody | DYR antibody | DYR_HUMAN antibody | EC 1.5.1.3 antibody | DHFR | DHFRP1 | Dihydrofolate Reductase | DYR | EC 1.5.1.3 |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. |
| Host species | Rabbit |
| Specificity | Dihydrofolate reductase |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within human DHFR. |
| Positive Control | WB: A549, HeLa, THP-1, HEK293, Jurkat, NIH/3T3 and C6 cell lysates. ICC/IF: A375 cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human Dihydrofolate reductase/DHFR Antibody, Recombinant, could be used for WB, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Shape | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Recombinant Dihydrofolate reductase/DHFR Antibody (Ab099867) - Western Blot
All lanes: Recombinant Dihydrofolate reductase/DHFR Antibody (Ab099867) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 23 kDa
Exposure time: 2.5 s
Recombinant Dihydrofolate reductase/DHFR Antibody (Ab099867) - IF/ICC
Immunofluorescent analysis of DHFR staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the Recombinant Dihydrofolate reductase/DHFR Antibody (Ab099867) in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin -AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
| Application | Dilution info |
|---|---|
| IF/ICC |
1/50-1/200
|
| WB |
1/500-1/1000
|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Jul 17, 2024 | Ab099867 | |
| Certificate of Analysis | Jul 17, 2024 | Ab099867 |