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Recombinant Dihydrofolate reductase/DHFR Antibody - Primary antibody, specific to Dihydrofolate reductase, Rabbit IgG

    Application:
  • IF/ICC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab099867
Grouped product items
SKU Size
Availability
Price Qty
Ab099867-10μl
10μl
2
$59.90
Ab099867-50μl
50μl
1
$189.90
Ab099867-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab099867-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

Recombinant; Rabbit anti Human Dihydrofolate reductase/DHFR Antibody; WB, IF/ICC; Unconjugated

Basic Description

Product Name Recombinant Dihydrofolate reductase/DHFR Antibody - Primary antibody, specific to Dihydrofolate reductase, Rabbit IgG
Synonyms DHFR antibody | DHFRP1 antibody | Dihydrofolate reductase antibody | DYR antibody | DYR_HUMAN antibody | EC 1.5.1.3 antibody | DHFR | DHFRP1 | Dihydrofolate Reductase | DYR | EC 1.5.1.3
Specifications & Purity ExactAb™, Validated, Recombinant, 0.5 mg/mL
Biochemical and Physiological Mechanisms Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
Host species Rabbit
Specificity Dihydrofolate reductase
Immunogen KLH-conjugated synthetic peptide encompassing a sequence within human DHFR.
Positive Control WB: A549, HeLa, THP-1, HEK293, Jurkat, NIH/3T3 and C6 cell lysates. ICC/IF: A375 cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Dihydrofolate reductase/DHFR Antibody, Recombinant, could be used for WB, IF/ICC and so on.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Immunogen affinity purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

DHFR Tclin Dihydrofolate reductase (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant Dihydrofolate reductase/DHFR Antibody (Ab099867) - Western Blot
All lanes: Recombinant Dihydrofolate reductase/DHFR Antibody (Ab099867) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 21 kDa
Observed band size: 23 kDa
Exposure time: 2.5 s

Recombinant Dihydrofolate reductase/DHFR Antibody (Ab099867) - IF/ICC
Immunofluorescent analysis of DHFR staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the Recombinant Dihydrofolate reductase/DHFR Antibody (Ab099867) in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin -AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Application

Application Dilution info
IF/ICC

1/50-1/200

WB

1/500-1/1000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0708400 Certificate of Analysis Jul 17, 2024 Ab099867
ZJ24F0708399 Certificate of Analysis Jul 17, 2024 Ab099867

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