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Recombinant CDT2/RAMP Antibody - Primary antibody, specific to DTL, Rabbit IgG

Grade & Purity:

1.3 mg/mL

Application:

Host species: Rabbit
Reactive species: Human,Mouse,Rat
Isotype: Rabbit IgG
Conjugation: Unconjugated

In stock

Item Number

Ab095882

Grouped product items
SKU	Size	Availability	Price
Ab095882-10μl	10μl	2	$79.90
Ab095882-50μl	50μl	1	$229.90
Ab095882-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$379.90
Ab095882-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,999.90

Recombinant; Rabbit anti Human CDT2/RAMP Antibody; WB, IHC; Unconjugated

View related series

Click to view target information: DTL

Accession#: Q9NZJ0 (1) CDT2 (1) DTL (1) Gene ID: 51514 (1) RAMP (1)

Basic Description

Product Name	Recombinant CDT2/RAMP Antibody - Primary antibody, specific to DTL, Rabbit IgG
Synonyms	DTL \| CDT2 \| RAMP \| DCAF2 \| L2DTL \| Lethal(2) denticleless protein homolog antibody \| CDW1 antibody \| DCAF2 antibody \| DDB1 and CUL4 associated factor 2 antibody \| Ddb1- and Cul4-associated factor 2 antibody \| Denticleless homolog antibody \| Denticleless
Specifications & Purity	ExactAb™, Validated, Recombinant, 1.3 mg/mL
Host species	Rabbit
Specificity	DTL
Immunogen	Recombinant protein of human CDT2
Positive Control	WB: HeLa, HEK293, K562, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human lung cancer tissue.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human CDT2/RAMP Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB:1/500-1/1000 IHC: 1/50-1/100 Protein Function: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen Affinity Purified
Shape	Liquid
Concentration	1.3 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

DTL Tbio Denticleless protein homolog (0 Activities)

Discover Target: DTL

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Western Blot: Recombinant CDT2/RAMP Antibody (Ab095882)

Recombinant CDT2/RAMP Antibody (Ab095882) - Western Blot
All lanes: Recombinant CDT2/RAMP Antibody (Ab095882) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 79 kDa
Observed band size: 95 kDa
Exposure time: 30.0 s

IHC: Recombinant CDT2/RAMP Antibody (Ab095882)

Recombinant CDT2/RAMP Antibody (Ab095882) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant CDT2/RAMP Antibody (Ab095882). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application	Dilution info
IHC	1/50 - 1/100
WB	1/500-1/1000

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0708925	Certificate of Analysis	Jul 29, 2024	Ab095882
ZJ24F0708924	Certificate of Analysis	Jul 29, 2024	Ab095882

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Recombinant CDT2/RAMP Antibody - Primary antibody, specific to DTL, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

Recombinant CDT2/RAMP Antibody - Primary antibody, specific to DTL, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews