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Recombinant Caspase-10/CASP-10 Antibody - Primary antibody, specific to Caspase-10/CASP-10, Rabbit IgG

    Application:
  • IF/ICC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab092983
Grouped product items
SKU Size
Availability
Price Qty
Ab092983-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab092983-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab092983-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$319.90
Ab092983-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,099.90

Recombinant; Rabbit anti Human Caspase-10/CASP-10 Antibody; WB, ICC, IF; Unconjugated

Basic Description

Product Name Recombinant Caspase-10/CASP-10 Antibody - Primary antibody, specific to Caspase-10/CASP-10, Rabbit IgG
Synonyms Apoptotic protease Mch-4 antibody | CASP 10 antibody | CASP-10 antibody | CASP10 antibody | CASPA_HUMAN antibody | Caspase 10 apoptosis related cysteine peptidase antibody | Caspase-10 antibody | Caspase-10 subunit p12 antibody | FADD like ICE2 antibody |
Specifications & Purity Recombinant, ExactAb™, Validated, See COA
Host species Rabbit
Specificity Caspase-10/CASP-10
Immunogen A synthetic peptide derived from human pro Caspase 10 (AA 200-260).
Positive Control WB: HeLa lysates. ICC/IF: MCF-7 cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Caspase-10/CASP-10 Antibody, Recombinant, could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Function:
Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp- -AMC and Asp-Glu-Val-Asp- -AMC. Isoform C is proteolytically inactive.

Product Properties

Isotype Rabbit IgG
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Caspase-10/CASP-10 Antibody (Ab092983) - Western Blot
All lanes: Recombinant Caspase-10/CASP-10 Antibody (Ab092983) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 59 kDa
Observed band size: 47, 64 kDa
The molecular weight of CASP10 observed on Western blots may vary depending on which of its isoform, truncation, or cleavage forms are present. Full-length CASP10 has a propeptide with 219 amino acid and 6 isoforms produced by alternative splicing.
During the processing of Caspase-10, the initial cleavage produces two intermediate products, namely p47 and p43. These intermediates are markers of Caspase-10 activation and can typically be observed 15-30 minutes after cells are stimulated by CD95L or TRAIL. Specifically, the cleavage of Caspase-10 occurs at specific amino acid sites, such as D219 and D372, leading to the release of active subunits (p23/p17 and p12) (PMID: 12198154).
Exposure time: 17.8 s

Recombinant Caspase-10/CASP-10 Antibody (Ab092983) - IF/ICC
Immunofluorescent analysis of MCF7 cells, using Recombinant Caspase-10/CASP-10 Antibody (Ab092983).

Application

Application Dilution info
IF/ICC

1/50-1/200

WB

1/500-1/1000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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