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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab092983-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$69.90
|
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Ab092983-50μl
|
50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$199.90
|
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Ab092983-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$319.90
|
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Ab092983-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,099.90
|
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Recombinant; Rabbit anti Human Caspase-10/CASP-10 Antibody; WB, ICC, IF; Unconjugated
| Product Name | Recombinant Caspase-10/CASP-10 Antibody - Primary antibody, specific to Caspase-10/CASP-10, Rabbit IgG |
|---|---|
| Synonyms | Apoptotic protease Mch-4 antibody | CASP 10 antibody | CASP-10 antibody | CASP10 antibody | CASPA_HUMAN antibody | Caspase 10 apoptosis related cysteine peptidase antibody | Caspase-10 antibody | Caspase-10 subunit p12 antibody | FADD like ICE2 antibody | |
| Specifications & Purity | Recombinant, ExactAb™, Validated, See COA |
| Host species | Rabbit |
| Specificity | Caspase-10/CASP-10 |
| Immunogen | A synthetic peptide derived from human pro Caspase 10 (AA 200-260). |
| Positive Control | WB: HeLa lysates. ICC/IF: MCF-7 cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human Caspase-10/CASP-10 Antibody, Recombinant, could be used for WB, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
Recombinant Caspase-10/CASP-10 Antibody (Ab092983) - Western Blot
All lanes: Recombinant Caspase-10/CASP-10 Antibody (Ab092983) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 47, 64 kDa
The molecular weight of CASP10 observed on Western blots may vary depending on which of its isoform, truncation, or cleavage forms are present. Full-length CASP10 has a propeptide with 219 amino acid and 6 isoforms produced by alternative splicing.
During the processing of Caspase-10, the initial cleavage produces two intermediate products, namely p47 and p43. These intermediates are markers of Caspase-10 activation and can typically be observed 15-30 minutes after cells are stimulated by CD95L or TRAIL. Specifically, the cleavage of Caspase-10 occurs at specific amino acid sites, such as D219 and D372, leading to the release of active subunits (p23/p17 and p12) (PMID: 12198154).
Exposure time: 17.8 s
Recombinant Caspase-10/CASP-10 Antibody (Ab092983) - IF/ICC
Immunofluorescent analysis of MCF7 cells, using Recombinant Caspase-10/CASP-10 Antibody (Ab092983).
| Application | Dilution info |
|---|---|
| IF/ICC |
1/50-1/200
|
| WB |
1/500-1/1000
|