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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab091243-10μl
|
10μl |
1
|
$69.90
|
|
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Ab091243-50μl
|
50μl |
1
|
$199.90
|
|
|
Ab091243-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$289.90
|
|
|
Ab091243-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,699.90
|
|
Recombinant; Rabbit anti Human Bid Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | Recombinant Bid Antibody - Primary antibody, specific to BID, Rabbit IgG |
|---|---|
| Synonyms | apoptic death agonist | BH3 interacting domain death agonist | BH3-interacting domain death agonist | BID isoform ES(1b) | BID isoform L(2) | BID isoform Si6 | BID | desmocollin type 4 | FP497 | Human BID coding sequence | MGC15319 | MGC42355 | p22 BID | |
| Specifications & Purity | Recombinant, ExactAb™, Validated, See COA |
| Biochemical and Physiological Mechanisms | The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2. |
| Host species | Rabbit |
| Specificity | BID |
| Immunogen | A synthetic peptide derived from human BID (AA 30-86) |
| Positive Control | WB: Jurkat, MOLT-4 and HEK293 cell lysates. IHC: Human lung tissue. IF/ICC: Jurkat cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human Bid Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Recombinant Bid Antibody (Ab091243) - Western Blot
All lanes: Recombinant Bid Antibody (Ab091243) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 20 kDa
Exposure time: 10.8 s
Recombinant Bid Antibody (Ab091243) - IF/ICC
Immunocytochemistry analysis of Bid (green) in Jurkat using Recombinant Bid Antibody (Ab091243) and DAPI (blue).
Recombinant Bid Antibody (Ab091243) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung tissue using Recombinant Bid Antibody (Ab091243). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Application | Dilution info |
|---|---|
| IF/ICC | 1/50 - 1/200 |
| WB |
1/1000-1/2000
|
| IHC | 1/100-1/200 |
Starting at $59.90
Starting at $59.90