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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab214361-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$59.90
|
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Ab214361-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$159.90
|
|
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Ab214361-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$254.90
|
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Ab214361-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,529.90
|
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mAb (F02/9A12); Mouse anti Human BID; WB, IF/ICC, ELISA; Unconjugated
| Product Name | BID Mouse mAb - Primary antibody, specific to Human BID |
|---|---|
| Synonyms | Apoptic death agonist antibody | Apoptotic death agonist BID antibody | BH3 interacting domain death agonist antibody | BH3 interacting domain death agonist p11 antibody | BH3 interacting domain death agonist p13 antibody | BH3 interacting domain death ag |
| Specifications & Purity | Animal Free, Carrier Free, ExactAb™, Azide Free, Validated, High Performance, ≥95%(SDS-PAGE), 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Induces caspases and apoptosis. Counters the protective effect of BCL2 (By similarity). BH3-interacting domain death agonist p15, Induces caspase activation and apoptosis. Allows the release of cytochrome c. Isoform 1, Induces ICE-like proteases and a |
| Host species | Mouse |
| Specificity | Human BID |
| Immunogen | Recombinant protein corresponding to Human BID |
| Positive Control | WB: Jurkat, HepG2, MCF-7, A549 and MOLT-4 whole cell lysates IF/ICC: HeLa cells |
| Conjugation | Unconjugated |
| Grade | Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Mouse anti Human BID Antibody, clone F02/9A12, could be used for WB, IF/ICC, ELISA and so on. |
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150.0 KDa |
| Purification Method | Protein A purified |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
BID Mouse mAb (Ab214361) - Western Blot
All lanes: BID Mouse Antibody (Ab214361) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 20 kDa
Exposure time: 60.0 s
BID Mouse mAb (Ab214361) - ICC/IF
IF analysis of BID (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with BID Mouse mAb (Ab214361) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| IF/ICC |
1/200
|
| WB | 1/1000 - 1/100000 |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Feb 28, 2025 | Ab214361 | |
| Certificate of Analysis | Feb 28, 2025 | Ab214361 |
Starting at $69.90
Starting at $59.90