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BID Mouse mAb - Primary antibody, specific to Human BID

    Application:
  • ELISA
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG2a
  • Clone Number: F02/9A12
  • Conjugation: Unconjugated
In stock
Item Number
Ab214361
Grouped product items
SKU Size
Availability
Price Qty
Ab214361-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
Ab214361-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$159.90
Ab214361-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$254.90
Ab214361-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,529.90

mAb (F02/9A12); Mouse anti Human BID; WB, IF/ICC, ELISA; Unconjugated

Basic Description

Product Name BID Mouse mAb - Primary antibody, specific to Human BID
Synonyms Apoptic death agonist antibody | Apoptotic death agonist BID antibody | BH3 interacting domain death agonist antibody | BH3 interacting domain death agonist p11 antibody | BH3 interacting domain death agonist p13 antibody | BH3 interacting domain death ag
Specifications & Purity Animal Free, Carrier Free, ExactAb™, Azide Free, Validated, High Performance, ≥95%(SDS-PAGE), 1.0 mg/mL
Biochemical and Physiological Mechanisms Induces caspases and apoptosis. Counters the protective effect of BCL2 (By similarity). BH3-interacting domain death agonist p15, Induces caspase activation and apoptosis. Allows the release of cytochrome c. Isoform 1, Induces ICE-like proteases and a
Host species Mouse
Specificity Human BID
Immunogen Recombinant protein corresponding to Human BID
Positive Control WB: Jurkat, HepG2, MCF-7, A549 and MOLT-4 whole cell lysates IF/ICC: HeLa cells
Conjugation Unconjugated
Grade Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti Human BID Antibody, clone F02/9A12, could be used for WB, IF/ICC, ELISA and so on.



Product Properties

Light Chain Type kappa
SDS-PAGE 150.0 KDa
Purification Method Protein A purified
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

BID Tbio BH3-interacting domain death agonist (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

BID Mouse mAb (Ab214361) - Western Blot
All lanes: BID Mouse Antibody (Ab214361) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 22 kDa
Observed band size: 20 kDa
Exposure time: 60.0 s 

BID Mouse mAb (Ab214361) - ICC/IF
IF analysis of BID (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with BID Mouse mAb (Ab214361) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

1/200

WB

1/1000 - 1/100000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ25F0218663 Certificate of Analysis Feb 28, 2025 Ab214361
ZJ25F0218662 Certificate of Analysis Feb 28, 2025 Ab214361

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