Grouped product items

SKU

Size

Availability

Price

Qty

Ab090774-10μl

10μl

Available within 8-12 weeks(?)

$69.90

Ab090774-50μl

50μl

Available within 8-12 weeks(?)

$199.90

Ab090774-100μl

100μl

Available within 8-12 weeks(?)

$309.90

Ab090774-1ml

1ml

Available within 8-12 weeks(?)

$2,199.90

Basic Description

Product Name	Recombinant Bcl-2 Antibody - Primary antibody, specific to Bcl-2, Rabbit IgG
Synonyms	Apoptosis regulator Bcl 2 antibody \| Apoptosis regulator Bcl-2 antibody \| Apoptosis regulator Bcl2 antibody \| AW986256 antibody \| B cell CLL/lymphoma 2 antibody \| B cell leukemia/lymphoma 2 antibody \| Bcl-2 antibody \| Bcl2 antibody \| BCL2_HUMAN antibody \|
Specifications & Purity	Recombinant, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms	Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. In
Host species	Rabbit
Specificity	Bcl-2
Immunogen	A synthetic peptide derived from human Bcl-2 (AA 48-81)
Positive Control	WB: HeLa, A375, Jurkat, MCF7, THP-1, U937, NIH/3T3 and C6 cell lysates. IHC: Rat liver, stomach, Human non-Hodgkin's lymphoma, squamous cell carcinoma, spleen, Mouse skin tissues. IF/ICC: Jurkat cells.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human Bcl-2 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

BCL2 Tclin Apoptosis regulator Bcl-2 (0 Activities)

Discover Target: BCL2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

BCL2 Tclin Apoptosis regulator Bcl-2 (3787 Activities)

Discover Target: BCL2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

BCL2 Tclin Apoptosis regulator BAX/Bcl-2 (1 Activities)

Discover Target: BCL2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

BCL2 Tclin Apoptosis regulator Bcl-2/Bcl-2 homologous antagonist/killer (28 Activities)

Discover Target: BCL2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

BCL2 Tclin Protein cereblon/Apoptosis regulator Bcl-2 (74 Activities)

Discover Target: BCL2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

BCL2 Tclin VHL/Apoptosis regulator Bcl-2 (38 Activities)

Discover Target: BCL2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

BCL2 Tclin BCL2/BCL2L11 (33 Activities)

Discover Target: BCL2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Western Blot: Recombinant Bcl-2 Antibody (Ab090774)

Recombinant Bcl-2 Antibody (Ab090774) - Western Blot
All lanes: Recombinant Bcl-2 Antibody (Ab090774) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 26 kDa
Observed band size: 17, 26, 59 kDa
Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (PubMed:25609812, PubMed:8183370).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Exposure time: 9.3 s