ExactAb™, Validated, Recombinant, High performance, 0.25mg/mL
Host species
Rabbit
Specificity
Bax
Immunogen
A synthetic peptide of human BAX (AA 1-100).
Positive Control
WB: A549, HT-29, HeLa, LNCaP, Hep G2 and HKE293 cell lysates. IHC: Human Ovarian cancer tissue. IF/ICC: HeLa cells. Flow: HeLa cells. IP: HT-29 whole cell lysate.
Conjugation
Unconjugated
Grade
ExactAb™, High Performance, Recombinant, Validated
Note
本产品即将售完下架，替代产品为 Ab090655
Product Description
Rabbit anti Human Bax Antibody, Recombinant (RR647), could be used for WB, IHC, Flow, IP and so on. Application: WB: 1/1000 – 1/2500 IHC: 1/1600-1/3200 Flow: 1/100-1/400 IP: 1/25
IF/ICC:1/1000 Protein Function Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Shape
Liquid
Concentration
0.25mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Recombinant Bax Antibody (Ab090652) - Western Blot All lanes: Recombinant Bax Antibody (Ab090652) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 21 kDa Observed band size: 20 kDa Exposure time: 6.2 s
Recombinant Bax Antibody (Ab090652) - IHC Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue labelling Bax with Recombinant Bax Antibody (Ab090652) at 1/1600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Recombinant Bax Antibody (Ab090652) - Flow Cytometry Overlay histogram showing HeLa cells stained with Recombinant Bax Antibody (Ab090652) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant Bax Antibody (Ab090652) (1/400 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
Recombinant Bax Antibody (Ab090652) - IP Bax was immunoprecipitated from 0.5 mg HT-29 whole cell lysate with Recombinant Bax Antibody (Ab090652) at 1/25 dilution. 2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant Bax Antibody (Ab090652) IP in HT-29 whole cell lysate Lane 2: PBS instead of Recombinant Bax Antibody (Ab090652) in HT-29 whole cell lysate Lane 3: HT-29 whole cell lysate, 10 μg (input)
Exposure: 30 s.
Recombinant Bax Antibody (Ab090652) - IF/ICC IF analysis of Recombinant Bax Antibody (Ab090652) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant Bax Antibody (Ab090652) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (FITC) (Ab170148) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
IF/ICC
1:1000
Flow Cytometry
1/100-1/400
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Find and download the COA for your product by matching the lot number on the packaging.
ZJ24F0404255
