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Recombinant ATP1A1 Antibody - Primary antibody, specific to ATP1A1, Rabbit IgG

COA COA
    Application:
  • Flow Cytometry
  • IHC
  • IP
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Clone Number: RR685
  • Conjugation: Unconjugated
In stock
Item Number
Ab128631
Grouped product items
SKU Size
Availability
 Price Qty
Ab128631-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$49.90
Ab128631-50μl
50μl
1
$147.90
Ab128631-100μl
100μl
2
$237.90
Ab128631-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,707.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

Recombinant (RR685); Rabbit anti Human ATP1A1 Antibody; WB, IHC, Flow, IP; Unconjugated

View related series
Click to view target information: ATP1A1
Accession#:P05023 (4) ATP1A1 (4) Flow Cytometry (1158) Gene ID:476 (4)

Basic Description

Product Name Recombinant ATP1A1 Antibody - Primary antibody, specific to ATP1A1, Rabbit IgG
Synonyms ATPase Na+/K+ transporting alpha antibody | adenosinetriphosphatase antibody | AT1A1_HUMAN antibody | ATP1A1 antibody | ATP1A4 antibody | ATP1AL2 antibody | ATP1B antibody | ATP1B1 antibody | ATPase Na+/K+ transporting alpha 1 polypeptide antibody | ATPas
Specifications & Purity ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL
Host species Rabbit
Specificity ATP1A1
Immunogen A synthetic peptide corresponding to residues on the N-terminus of human NaK ATPase was used as an immunogen.
Positive Control WB: C6, HT29, A431, Hep G2, and Raw264.7 cell lysates. IHC: Endometrium tissue. Flow: HeLa cells. IP: TT whole cell lysate.
Conjugation Unconjugated
Grade ExactAb™, High Performance, Recombinant, Validated
Product Description

Rabbit anti Human ATP1A1 Antibody, Recombinant antibody (RR685), could be used for WB, IHC, Flow, IP and so on.

Application:

WB: 1/2000 - 1/5000       

IHC: 1/10000 - 1/20000

Flow: 1/50 - 1/200

IP: 1/50

Protein Function

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Post-translational

Phosphorylation on Tyr-10 modulates pumping activity.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

ATP1A1 Tclin Sodium/potassium-transporting ATPase subunit alpha-1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
ATP1A1 Tclin Sodium/potassium-transporting ATPase alpha-1 chain (7 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant ATP1A1 Antibody (Ab128631) - Western Blot
All lanes: Recombinant ATP1A1 Antibody (Ab128631) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 100 kDa
Observed band size: 97 kDa
Exposure time: 32.2 s

Recombinant ATP1A1 Antibody (Ab128631) - Western Blot
All lanes: Recombinant ATP1A1 Antibody (Ab128631) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 100 kDa
Observed band size: 97 kDa
Exposure time: 10.0 s

Recombinant ATP1A1 Antibody (Ab128631) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of endometrium tissue labelling NaK ATPase with Recombinant ATP1A1 Antibody (Ab128631) at 1/10000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Recombinant ATP1A1 Antibody (Ab128631) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Recombinant ATP1A1 Antibody (Ab128631) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant ATP1A1 Antibody (Ab128631) (1/200 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.

Recombinant ATP1A1 Antibody (Ab128631) - IP
NaK ATPase was immunoprecipitated from 0.4 mg of TT whole cell lysate with Recombinant ATP1A1 Antibody (Ab128631) at 1/50 dilution
2nd Ab: GAR HRP for IP 1/500

Lane 1: Recombinant ATP1A1 Antibody (Ab128631) IP in TT whole cell lysate
Lane 2: PBS instead of Recombinant ATP1A1 Antibody (Ab128631) in TT whole cell lysate
Lane 3: TT whole cell lysate, 10 μg (input)

Exposure: 120 s.

Application

Application Dilution info
IP

1/50

WB

1/2000 - 1/5000

Flow Cytometry

1/50 - 1/200

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0901094 Certificate of Analysis Sep 18, 2023 Ab128631
ZJ23F0901095 Certificate of Analysis Sep 18, 2023 Ab128631

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Documents & Articles

Flow Cytometry
Categories: Technical articles
Tags:
Flow Cytometry
Flow cytometry analysis
Categories: Technical articles
Tags:
Forward scatter Side scatter Dichroic filters/mirrors The multicolor flow cytometer
Flow cytometry protocol
Categories: Protocols
Tags:
Antibodies Flow Cytometry Flow Antibodies Reactive dyes Indirect antibody labelling
Immunoprecipitation (IP) lysates and reagents
Categories: Protocols
Tags:
Immunoprecipitation lysate Tissue lysate
Immunohistochemical staining protocol for frozen samples (IHC-Fr)
Categories: Protocols
Tags:
IHC Monoclonal antibodies Immunohistochemistry Frozen section Recombinant Antibodies
Immunohistochemistry (IHC) staining antigen retrieval protocol
Categories: Protocols
Tags:
IHC Monoclonal antibodies Immunohistochemistry Recombinant Antibodies Antigen Retrieval
General Immunoprecipitation Protocol
Categories: Protocols
Tags:
Immunoprecipitation Western blot Cell lysate Co-immunoprecipitation IP Protein interaction Protein A/G magnetic beads
RNA Immunoprecipitation (RIP) Protocol
Categories: Protocols
Tags:
IP Protein A/G magnetic beads RNA immunoprecipitation RIP RNA protein interaction Isotype control antibody Input Crosslinking
Immobilized IP Protocol
Categories: Protocols
Tags:
Immunoprecipitation WB Western blot IP Protein interaction Immobilization immunoprecipitation Immobilized antibody
Flow Cytometry Using Methanol Permeabilization
Categories: Protocols
Tags:
Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

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