This is a demo store. No orders will be fulfilled.

Recombinant ATG3 Antibody - Primary antibody, specific to ATG3, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab089994
Grouped product items
SKU Size
Availability
Price Qty
Ab089994-10μl
10μl
1
$69.90
Ab089994-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$189.90
Ab089994-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$289.90
Ab089994-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,499.90

Recombinant; Rabbit anti Human ATG3 Antibody; WB, IHC, ICC, IF; Unconjugated

Basic Description

Product Name Recombinant ATG3 Antibody - Primary antibody, specific to ATG3, Rabbit IgG
Synonyms APG3-like | Autophagy-related protein 3 | EC 2.3.2.- | hApg3 | Protein PC3-96 | 2610016C12Rik antibody | Apg 3 antibody | APG3 antibody | APG3 autophagy 3 like antibody | APG3 like antibody | APG3, S. cerevisiae, homolog of antibody | APG3-like antibody |
Specifications & Purity Recombinant, ExactAb™, Validated, See COA
Host species Rabbit
Specificity ATG3
Immunogen A synthetic peptide derived from human ATG3 (AA 2-20)
Positive Control WB: K562, HeLa, THP-1, Jurkat, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human tonsil tissue. ICC/IF: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human ATG3 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function:
E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.

Product Properties

Isotype Rabbit IgG
Light Chain Type kappa
SDS-PAGE 150kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

ATG3 Tbio Ubiquitin-like-conjugating enzyme ATG3 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant ATG3 Antibody (Ab089994) - Western Blot
All lanes: Recombinant ATG3 Antibody (Ab089994) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 49.5 s

Recombinant ATG3 Antibody (Ab089994) - IF/ICC
Immunocytochemistry analysis of ATG3 (green) in HeLa using Recombinant ATG3 Antibody (Ab089994) and DAPI (blue).

Recombinant ATG3 Antibody (Ab089994) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant ATG3 Antibody (Ab089994). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

Application Dilution info
WB

1/500-1/1000

IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0809166 Certificate of Analysis Aug 05, 2024 Ab089994
ZJ24F0809165 Certificate of Analysis Aug 05, 2024 Ab089994

Solution Calculators

Reviews

Customer Reviews

Shall we send you a message when we have discounts available?

Remind me later

Thank you! Please check your email inbox to confirm.

Oops! Notifications are disabled.