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PKM2 Antibody - Primary antibody, specific to PKM

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PKM2 Antibody - Primary antibody, specific to PKM

Grade & Purity:

High Performance

See COA

Application:

IF/ICC
IHC
WB

Host species: Rabbit
Reactive species: Human,Mouse,Rat
Conjugation: Unconjugated

In stock

Item Number

Ab122149

Grouped product items
SKU	Size	Availability	Price	Qty
Ab122149-10μl	10μl	10	$59.90
Ab122149-50μl	50μl	4	$169.90
Ab122149-100μl	100μl	2	$279.90
Ab122149-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,999.90

pAb; Rabbit anti Human PKM2 Antibody; WB, IHC, IF/ICC; Unconjugated

View related series

Click to view target information: PKM

Accession#:P14618 (2) Gene ID:5315 (2) PKM (3) PKM2 (2)

Basic Description

Product Name	PKM2 Antibody - Primary antibody, specific to PKM
Synonyms	CTHBP \| Cytosolic thyroid hormone-binding protein \| MGC3932 \| OIP3 \| OIP-3 \| OIP3EC 2.7.1.40 \| Opa-interacting protein 3 \| p58 \| PK, muscle type \| PK2 \| PK3 \| PK3PKM \| PKM2 \| Pyruvate kinase 2/3 \| pyruvate kinase isozymes M1/M2 \| Pyruvate kinase muscle is
Specifications & Purity	Carrier Free, ExactAb™, Azide Free, Validated, High Performance, KD Validation, See COA
Biochemical and Physiological Mechanisms	Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ra
Host species	Rabbit
Specificity	PKM
Immunogen	Recombinant PKM2 expressed in E.coli (AA 141-248).
Positive Control	WB: K562, MCF7, HeLa, Hep G2, Raji, Ramos, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer, skin cancer, prostate, prostate cancer, colorectal cancer, liver cancer, Mouse lung, testis, kidney, oviduct tissues. IF/ICC: MCF7, HeLa cells.
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, High Performance, KD Validation, Validated
Product Description	Rabbit anti Human PKM2 Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.

Product Properties

SDS-PAGE	150 kDa
Purification Method	Protein A purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

PKM Tchem Pyruvate kinase PKM (0 Activities)

Discover Target: PKM

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Western Blot: PKM2 Antibody (Ab122149)

PKM2 Antibody (Ab122149) - Western Blot
All lanes: PKM2 Antibody (Ab122149) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 58 kDa
Observed band size: 61 kDa
Exposure time: 3.7 s

IF/ICC: PKM2 Antibody (Ab122149)

PKM2 Antibody (Ab122149) - IF/ICC
FITC staining on IF
Samples: Human MCF7 cell
Primary Ab: 20 μg/mL PKM2 Antibody (Ab122149)
Second Ab: 1 μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM2 Antibody (Ab122149)

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM2 Antibody (Ab122149)

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Human Skin cancer Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM2 Antibody (Ab122149)

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Human Prostate Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM2 Antibody (Ab122149)

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Human Prostate cancer Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM2 Antibody (Ab122149)

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Human Colorectal cancer Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM2 Antibody (Ab122149)

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Western Blot: PKM2 Antibody (Ab122149)

PKM2 Antibody (Ab122149) - Western Blot
Lane 1: Wild-type MCF7 cell lysateLane 2: PKM2 knockout MCF7 cell lysatePredicted MW: 56,58 kDaObserved MW: 60 kDaPrimary Ab: 2 µg/mL PKM2 Antibody (Ab122149) Second Ab: 0.2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IF/ICC: PKM2 Antibody (Ab122149)

PKM2 Antibody (Ab122149) - IF/ICC
AF488 staining on IF
Samples: HeLa cell
Primary Ab: 20 μg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 μg/mL AF488-Linked Caprine Anti-Rabbit IgG Polyclonal
Antibody.

IHC: PKM2 Antibody (Ab122149)

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Mouse Lung Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Control: Used PBS instead of PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM2 Antibody (Ab122149)

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Mouse Testis Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Control: Used PBS instead of PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM2 Antibody (Ab122149)

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Mouse Oviduct Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Control: Used PBS instead of PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM2 Antibody (Ab122149)

PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Mouse Oviduct Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Control: Used PBS instead of PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Application

Application	Dilution info
IHC	5-20 µg/mL
WB	0.01-2 µg/mL
IF/ICC	5-20µg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0102507	Certificate of Analysis	Jan 17, 2024	Ab122149
ZJ24F0102506	Certificate of Analysis	Jan 17, 2024	Ab122149
ZJ24F0102505	Certificate of Analysis	Jan 16, 2024	Ab122149

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