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PKM Antibody - Primary antibody, specific to PKM, Rabbit IgG

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PKM Antibody - Primary antibody, specific to PKM, Rabbit IgG

Grade & Purity:

High Performance

See COA

Application:

IF/ICC
IHC
WB

Host species: Rabbit
Reactive species: Human,Mouse,Rat
Isotype: Rabbit IgG
Conjugation: Unconjugated

In stock

Item Number

Ab122143

Grouped product items
SKU	Size	Availability	Price	Qty
Ab122143-10μl	10μl	5	$56.90
Ab122143-50μl	50μl	3	$169.90
Ab122143-100μl	100μl	2	$279.90
Ab122143-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,299.90

pAb; Rabbit anti Human PKM Antibody; WB, IHC, ICC, IF; Unconjugated

View related series

Click to view target information: PKM

Accession#:P14618 (2) Gene ID:5315 (2) PKM (3) PKM2 (2)

Basic Description

Product Name	PKM Antibody - Primary antibody, specific to PKM, Rabbit IgG
Synonyms	CTHBP antibody \| Cytosolic thyroid hormone-binding protein antibody \| KPYM_HUMAN antibody \| OIP-3 antibody \| Opa-interacting protein 3 antibody \| p58 antibody \| pkm antibody \| PKM1 antibody \| PKM2 antibody \| Pyruvate kinase 2/3 antibody \| Pyruvate kinase
Specifications & Purity	Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Host species	Rabbit
Specificity	PKM
Immunogen	Recombinant PKM2 expressed in E.coli (AA 141-248)
Positive Control	WB: K562, MCF7, HeLa, HepG2, A431, THP-1, Raji, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue; Human skin cancer tissue, Human prostate tissue, Human prostate cancer tissue, Human colorectal cancer tissue, Human liver cancer tissue. ICC/IF
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description	Rabbit anti Human PKM Antibody, Polyclonal, could be used for WB, IHC, ICC/IF and so on. Application WB: 0.01-2 µg/mL IHC: 5-20 µg/mL ICC/IF: 5-20 μg/mL Protein Function Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified and Protein A purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

PKM Tchem Pyruvate kinase PKM (0 Activities)

Discover Target: PKM

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Western Blot: PKM Antibody (Ab122143)

PKM Antibody (Ab122143) - Western Blot
All lanes: PKM Antibody (Ab122143) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 1.6 s

IF/ICC: PKM Antibody (Ab122143)

PKM Antibody (Ab122143) - IF/ICC
FITC staining on IF
Samples: Human MCF7 cells
Primary Ab: 20 ug/mL PKM Antibody (Ab122143)
Second Ab: 1 μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM Antibody (Ab122143)

PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM Antibody (Ab122143)

PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Skin cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM Antibody (Ab122143)

PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Prostate Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM Antibody (Ab122143)

PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Prostate cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM Antibody (Ab122143)

PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Colorectal cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

IHC: PKM Antibody (Ab122143)

PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Application

Application	Dilution info
IHC	5-20 µg/mL
WB	0.01-2 µg/mL
IF/ICC	5-20 µg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number	Certificate Type	Date	Item
ZJ23F1001237	Certificate of Analysis	Oct 10, 2023	Ab122143
ZJ23F1001236	Certificate of Analysis	Oct 10, 2023	Ab122143

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