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HAO1/GOX Antibody - Primary antibody, specific to HAO1/GOX, Rabbit IgG

    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab106852
Grouped product items
SKU Size
Availability
Price Qty
Ab106852-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab106852-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$189.90
Ab106852-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$249.90
Ab106852-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

pAb; Rabbit anti Human HAO1/GOX Antibody; WB, IHC, ICC, IF; Unconjugated

Basic Description

Product Name HAO1/GOX Antibody - Primary antibody, specific to HAO1/GOX, Rabbit IgG
Synonyms Glycolate oxidase antibody | (S) 2 hydroxy acid oxidase antibody | GOX antibody | GOX1 antibody | HAO1 antibody | Hydroxyacid Oxidase-1 | Hydroxyacid Oxidase1 | hydroxyacid oxidase 1 | HAOX1 antibody | HAOX1_HUMAN antibody | Hydroxyacid oxidase 1 antibody
Specifications & Purity ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host species Rabbit
Specificity HAO1/GOX
Immunogen Recombinant full length protein of human HAO1.
Positive Control WB: Mouse and Rat liver lysates. IHC: Human liver cancer tissue. ICC/IF: HEK293T cells.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Rabbit anti Human HAO1/GOX Antibody, Polyclonal, could be used for WB, IHC, ICC/IF and so on.
Application
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.

Product Properties

Isotype Rabbit IgG
SDS-PAGE 150 kDa
Purification Method Immunogen affinity purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

HAO1 Tclin Hydroxyacid oxidase 1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

HAO1/GOX Antibody (Ab106852) - Western Blot
All lanes: HAO1/GOX Antibody (Ab106852) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 40 kDa
Exposure time: 120.0 s

HAO1/GOX Antibody (Ab106852) - IHC
Immunohistochemical analysis of HAO1/GOX staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with HAO1/GOX Antibody (Ab106852) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

HAO1/GOX Antibody (Ab106852) - IF/ICC
Immunofluorescent analysis of HAO1/GOX staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with HAO1/GOX Antibody (Ab106852) in 3% BSA-PBS and incubated overnight at 4 °C in a humidity chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Application

Application Dilution info
WB

1/500-1/1000

IF/ICC

1/50-1/200

IHC

1/50 - 1/100

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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