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Recombinant GATA4 Antibody - Primary antibody, specific to GATA4, Rabbit IgG

    Application:
  • IF/ICC
  • WB
  • Host species: Rabbit
  • Reactive species: Human,Mouse,Rat
  • Isotype: Rabbit IgG
  • Conjugation: Unconjugated
In stock
Item Number
Ab104893
Grouped product items
SKU Size
Availability
Price Qty
Ab104893-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab104893-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab104893-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab104893-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,499.90

Recombinant; Rabbit anti Human GATA4 Antibody; WB, IF/ICC; Unconjugated

Basic Description

Product Name Recombinant GATA4 Antibody - Primary antibody, specific to GATA4, Rabbit IgG
Synonyms ASD2 | TOF | VSD1 | TACHD | GATA binding protein 4 | VSD1 antibody | GATA4 | GATA-4 | GATA-binding factor 4 | GATA-binding protein 4 | MGC126629 | transcription factor GATA-4 | ASD2 antibody | GATA 4 antibody | GATA binding protein 4 antibody | GATA-bindi
Specifications & Purity Recombinant, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity).
Host species Rabbit
Specificity GATA4
Immunogen A synthetic peptide derived from human GATA4 (AA 5-48)
Positive Control WB: Hep G2, HEK293 and HeLa cell lysates. IF/ICC: Hep G2 cells.
Conjugation Unconjugated
Grade ExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human GATA4 Antibody, Recombinant, could be used for WB, IF/ICC and so on.

Product Properties

Isotype Rabbit IgG
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

GATA4 Tbio Transcription factor GATA-4 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Recombinant GATA4 Antibody (Ab104893) - Western Blot
All lanes: Recombinant GATA4 Antibody (Ab104893) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/5000 dilution
Predicted band size: 45 kDa
Observed band size: 22, 57 kDa
~20 kDa band is consistent with what has been described in the literature (PMID: 25501827). Caspase-1 mediated cleavage of GATA4 generates a truncated protein that retains the ability to bind DNA but lacks transcriptional activation domains and acts as a dominant negative regulator of GATA4.
Exposure time: 167.7 s

Recombinant GATA4 Antibody (Ab104893) - IF/ICC
Immunofluorescent analysis of Hep G2 cells, using Recombinant GATA4 Antibody (Ab104893).

Application

Application Dilution info
WB

1/500-1/2000

IF/ICC

1/50 - 1/200

ICC/IF

1/100-1/500

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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