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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab104893-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$69.90
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Ab104893-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$199.90
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Ab104893-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$309.90
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Ab104893-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,499.90
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Recombinant; Rabbit anti Human GATA4 Antibody; WB, IF/ICC; Unconjugated
| Product Name | Recombinant GATA4 Antibody - Primary antibody, specific to GATA4, Rabbit IgG |
|---|---|
| Synonyms | ASD2 | TOF | VSD1 | TACHD | GATA binding protein 4 | VSD1 antibody | GATA4 | GATA-4 | GATA-binding factor 4 | GATA-binding protein 4 | MGC126629 | transcription factor GATA-4 | ASD2 antibody | GATA 4 antibody | GATA binding protein 4 antibody | GATA-bindi |
| Specifications & Purity | Recombinant, ExactAb™, Validated, See COA |
| Biochemical and Physiological Mechanisms | Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). |
| Host species | Rabbit |
| Specificity | GATA4 |
| Immunogen | A synthetic peptide derived from human GATA4 (AA 5-48) |
| Positive Control | WB: Hep G2, HEK293 and HeLa cell lysates. IF/ICC: Hep G2 cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description |
Rabbit anti Human GATA4 Antibody, Recombinant, could be used for WB, IF/ICC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Recombinant GATA4 Antibody (Ab104893) - Western Blot
All lanes: Recombinant GATA4 Antibody (Ab104893) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/5000 dilution
Predicted band size: 45 kDa
Observed band size: 22, 57 kDa
~20 kDa band is consistent with what has been described in the literature (PMID: 25501827). Caspase-1 mediated cleavage of GATA4 generates a truncated protein that retains the ability to bind DNA but lacks transcriptional activation domains and acts as a dominant negative regulator of GATA4.
Exposure time: 167.7 s
Recombinant GATA4 Antibody (Ab104893) - IF/ICC
Immunofluorescent analysis of Hep G2 cells, using Recombinant GATA4 Antibody (Ab104893).
| Application | Dilution info |
|---|---|
| WB | 1/500-1/2000 |
| IF/ICC | 1/50 - 1/200 |
| ICC/IF | 1/100-1/500 |