Skip to the beginning of the images gallery

Cell adhesion molecule 4 Antibody - Primary antibody, specific to CADM4, Rabbit IgG

Grade & Purity:

1.0 mg/mL

Application:

IF/ICC
IHC
WB

Host species: Rabbit
Reactive species: Human,Mouse,Rat
Isotype: Rabbit IgG
Conjugation: Unconjugated

In stock

Item Number

Ab095999

Grouped product items
SKU	Size	Availability	Price
Ab095999-10μl	10μl	1	$89.90
Ab095999-50μl	50μl	1	$269.90
Ab095999-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$379.90
Ab095999-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,999.90

pAb; Rabbit anti Human Cell adhesion molecule 4 Antibody; WB, IHC, IF, ICC; Unconjugated

View related series

Click to view target information: CADM4

Accession#: Q8NFZ8 (1) CADM4 (1) Cell adhesion molecule 4 (1) Gene ID: 199731 (1)

Basic Description

Product Name	Cell adhesion molecule 4 Antibody - Primary antibody, specific to CADM4, Rabbit IgG
Synonyms	TSLL2 antibody \| TSLC1-like protein 2 antibody \| TSLC1 like protein 2 antibody \| CADM4_HUMAN \| cadm4 \| Cell adhesion molecule 4 \| IgSF4C \| Immunoglobulin superfamily member 4C \| TSLC1 like 2 antibody \| synCAM4 antibody \| Nectin-like protein 4 antibody \| N
Specifications & Purity	ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host species	Rabbit
Specificity	CADM4
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CADM4.
Positive Control	WB: Mouse brain, Rat brain, U-2 OS and C6 lysates. IHC: Human brain tissue. IF/ICC: A549 cells.
Conjugation	Unconjugated
Grade	Carrier Free, ExactAb™, Validated
Product Description	Rabbit anti Human Cell adhesion molecule 4 Antibody, Polyclonal, could be used for WB, IHC, IF, ICC and so on. Application WB: 1/500-1/1000 IHC: 1/50 - 1/100 IF/IC :1/50 - 1/200 Protein Function Involved in the cell-cell adhesion. Has calcium- and magnesium-independent cell-cell adhesion activity. May have tumor-suppressor activity.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Immunogen affinity purified
Shape	Liquid
Concentration	1.0 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

CADM4 Tbio Cell adhesion molecule 4 (0 Activities)

Discover Target: CADM4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Western Blot: Cell adhesion molecule 4 Antibody (Ab095999)

Cell adhesion molecule 4 Antibody (Ab095999) - Western Blot
All lanes: Cell adhesion molecule 4 Antibody (Ab095999) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 43 kDa
Observed band size: 56, 72 kDa
The expression profile and molecular weight observed, which is due to glycosylation.
Exposure time: 0.3 s

IF/ICC: Cell adhesion molecule 4 Antibody (Ab095999)

Cell adhesion molecule 4 Antibody (Ab095999) - IF/ICC
Immunofluorescent analysis of CADM4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Cell adhesion molecule 4 Antibody (Ab095999) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IHC: Cell adhesion molecule 4 Antibody (Ab095999)

Cell adhesion molecule 4 Antibody (Ab095999) - IHC
Immunohistochemical analysis of CADM4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Cell adhesion molecule 4 Antibody (Ab095999) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Application

Application	Dilution info
IHC	1/50 - 1/100
IF/ICC	1/50 - 1/200
WB	1/500-1/1000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Cell adhesion molecule 4 Antibody - Primary antibody, specific to CADM4, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

Cell adhesion molecule 4 Antibody - Primary antibody, specific to CADM4, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews