Grouped product items

SKU

Size

Availability

Price

Qty

Ab093310-10μl

10μl

Available within 8-12 weeks(?)

$69.90

Ab093310-50μl

50μl

Available within 8-12 weeks(?)

$189.90

Ab093310-100μl

100μl

Available within 8-12 weeks(?)

$309.90

Ab093310-1ml

1ml

Available within 8-12 weeks(?)

$1,999.90

Basic Description

Product Name	CBP80 Antibody - Primary antibody, specific to NCBP1, Rabbit IgG
Synonyms	NCBP1 \| CBP80 \| NCBP \| Sto1 \| 80 kDa nuclear cap-binding protein antibody \| CBP80 antibody \| MGC2087 antibody \| NCBP 80 kDa subunit antibody \| NCBP antibody \| ncbp1 antibody \| NCBP1_HUMAN antibody \| nuclear cap binding protein subunit 1 antibody \| nuclear
Specifications & Purity	ExactAb™, Validated, 1.0 mg/mL
Host species	Rabbit
Specificity	NCBP1
Immunogen	A synthetic peptide derived from human NCBP1 (AA 1-50)
Positive Control	WB: MCF7, A431 and NIH/3T3 cell lysates. IHC: Human small intestinal carcinoma tissue.
Conjugation	Unconjugated
Grade	ExactAb™, Validated
Product Description	Rabbit anti Human CBP80 Antibody, Polyclonal, could be used for WB, IHC and so on. Application: WB: 1/500-1/2000 IHC: 1/100-1/300 Protein Function： Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Immunogen affinity purified
Shape	Liquid
Concentration	1.0 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

NCBP1 Tbio Nuclear cap-binding protein subunit 1 (0 Activities)

Discover Target: NCBP1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Western Blot: CBP80 Antibody (Ab093310)

CBP80 Antibody (Ab093310) - Western Blot
All lanes: CBP80 Antibody (Ab093310) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 80 kDa
Observed band size: 85 kDa
Exposure time: 100.0 s

IHC: CBP80 Antibody (Ab093310)

CBP80 Antibody (Ab093310) - IHC
Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. CBP80 Antibody (Ab093310) was diluted at 1/200 (4℃, overnight). Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). Secondary antibody was diluted at 1/200.

Application

Application	Dilution info
IHC	1/100-1/300
WB	1/500-1/2000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

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CBP80 Antibody - Primary antibody, specific to NCBP1, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

SKU	Size	Availability	Price
Ab093310-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$69.90
Ab093310-50μl	50μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$189.90
Ab093310-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$309.90
Ab093310-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,999.90

CBP80 Antibody - Primary antibody, specific to NCBP1, Rabbit IgG

Basic Description

Product Properties

Associated Targets(Human)

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews