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Goat Anti-Rabbit IgG H&L (AF594) - Secondary antibody, IgG

1 Citations COA COA
    Application:
  • ELISA
  • Flow Cytometry
  • IF/ICC
  • Host species: Goat
  • Reactive species: Rabbit
  • Isotype: Goat IgG
  • Conjugation: AF594
In stock
Item Number
Ab138214
Grouped product items
SKU Size
Availability
 Price Qty
Ab138214-100μl
100μl
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$87.90
Ab138214-1ml
1ml
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$327.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

pAb; Goat Anti-Rabbit IgG H&L (AF594); ICC, IF, IHC, Flow, ELISA

View related series
AF594 (3) CLARITY tissue clearing technology (64) Flow Cytometry (1158) Goat Anti-Rabbit (19)

Basic Description

Product Name Goat Anti-Rabbit IgG H&L (AF594) - Secondary antibody, IgG
Synonyms Goat Anti-Rabbit IgG H&L (AF594) | Goat Anti Rabbit IgG H&L (AF594) | Goat Anti-Rabbit IgG H&L-AF594 | Goat Anti Rabbit IgG H&L-AF594 | AF594-Goat Anti-Rabbit IgG H&L | AF594-Goat Anti Rabbit IgG H&L
Specifications & Purity ExactAb™, High Performance, Validated, Ex:590nm, Em:618nm, 1 mg/mL
Host species Goat
Immunogen Rabbit IgG
Conjugation AF594
Excitation(nm) Yellow-Green Laser(594nm)
Emission(nm) 618nm
Ex/Em(nm) Ex:590nm;Em:618nm
Grade Em:618nm, Ex:590nm, ExactAb™, High Performance, Validated
Product Description

Goat Anti-Rabbit IgG H&L (AF594), Secondary antibody, could be used for ICC, IF, IHC, Flow, ELISA and so on.
Background:
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 150 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Product Properties

Isotype IgG
Purification Method Antigen Affinity Purified
Shape Liquid
Concentration 1 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Images

Goat Anti-Rabbit IgG H&L (AF594) (Ab138214) - ICC/IF
IF analysis of HeLa cells labeling PABPN1 with purified PABPN1 Rabbit mAb (Ab119979) at 1/200 dilution. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were counterstained with beta Actin Mouse mAb (Ab155822) at 1/500 dilution - Goat Anti-Mouse IgG H&L (AF488) 1/1000. Goat Anti-Rabbit IgG H&L (AF594) (Ab138214) was used as the secondary antibody at 1/500 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

1/100-1/1000

Flow Cytometry

1/500-1/1000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0700547 Certificate of Analysis Mar 26, 2024 Ab138214
ZJ23F0700539 Certificate of Analysis Mar 26, 2024 Ab138214

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Documents & Articles

Antibody structure and isotypes
Categories: Technical articles
Tags:
Immunoglobulin Antibody isotype Heavy chain and light chain
Flow Cytometry
Categories: Technical articles
Tags:
Flow Cytometry
Flow cytometry analysis
Categories: Technical articles
Tags:
Forward scatter Side scatter Dichroic filters/mirrors The multicolor flow cytometer
How to choose and use antibodies-primary antibody?
Categories: Technical articles
Tags:
Recombinant antibody Knockout gene ICC
How to find the right secondary antibody?
Categories: Technical articles
Tags:
Fluorophore Direct/Indirect Detection Enzyme markers
Antibody applications and techniques
Categories: Technical articles
Tags:
ICC ELISA WB IHC
Flow cytometry protocol
Categories: Protocols
Tags:
Antibodies Flow Cytometry Flow Antibodies Reactive dyes Indirect antibody labelling
Immunocytochemistry (ICC) protocol
Categories: Technical articles
Tags:
Antibody Biological buffer Fluorescent dyes Immunochemical staining Indirect antibody labeling
Immunohistochemical staining protocol for frozen samples (IHC-Fr)
Categories: Protocols
Tags:
IHC Monoclonal antibodies Immunohistochemistry Frozen section Recombinant Antibodies
RNA dot blot protocol
Categories: Protocols
Tags:
Protein Electrophoresis & Western Blotting Dot Blotting Primary antibodies Secondary Antibodies
Immunohistochemistry (IHC) staining antigen retrieval protocol
Categories: Protocols
Tags:
IHC Monoclonal antibodies Immunohistochemistry Recombinant Antibodies Antigen Retrieval
In-cell ELISA protocol
Categories: Protocols
Tags:
Monoclonal antibodies In-cell ELISA Cytoblot Protein quantification HRP-Conjugated Secondary Antibodies Polyclonal Antibodies
Caspase Immunofluorescence Staining Protocol
Categories: Protocols
Tags:
Secondary antibody Apoptosis Programmed Death Immunofluorescence Caspase
General Protocol for Western Blotting
Categories: Protocols
Tags:
Western blotting WB Protein Electrophoresis & Western Blotting Biological buffers Immunoblotting Conjugated Secondary Antibodies HRP
Western Blotting with Fluorescently Labeled Antibodies
Categories: Protocols
Tags:
Protein Electrophoresis & Western Blotting Biological buffers Immunoblotting Fluorescently Labeled Antibodies Membrane Transfer
Immunofluorescence Staining Protocol (Methanol permeabilization)
Categories: Protocols
Tags:
Frozen section Immunofluorescence staining Tissue section Methanol permeability IF
Guidelines of Secondary Antibody for Western Blotting
Categories: Protocols
Tags:
Secondary antibody WB Western blot HRP Horseradish peroxidase Secondary Antibody Selection Guide Alexa Fluor
Flow Cytometry Using Methanol Permeabilization
Categories: Protocols
Tags:
Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection
CLARITY Tissue Clearing Staining Technology
Categories: Protocols
Tags:
CLARITY tissue clearing technology CLARITY tissue clearing lipid removal biological tissue 3D imaging Hydrogel
Fixation and Permeabilization Protocol for Transcription Factor Flow Cytometry
Categories: Protocols
Tags:
Flow Cytometry Transcription Factors Transcription factor flow cytometry Cell fixation and permeabilization Cell fixation Cell permeabilization FOXP3

Citations of This Product

How to Cite Aladdin Scientific Products?
1. Xu Tao, Yang Changwei, Lu Yang, Wang Heng, Chen Cheng, Zhou Yuchen, Chen Xiaoqing.  (2024)  Photocrosslinkable human amniotic membrane hydrogel for recovery from spinal cord injury.  Bio-Design and Manufacturing,    (1-18). 

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