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Donkey Anti-Rabbit IgG H&L (AF647) - Secondary antibody, specific to , IgG

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • Host species: Donkey
  • Reactive species: Rabbit
  • Isotype: Donkey IgG
  • Conjugation: AF647
In stock
Item Number
Ab136882
Grouped product items
SKU Size
Availability
 Price Qty
Ab136882-100μg
100μg
2
$69.90
Ab136882-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$447.90
Ab136882-500μg
500μg
1
$237.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

pAb; Donkey Anti-Rabbit IgG H&L (AF647); Flow, IF, ICC

View related series
AF647 (119) CLARITY tissue clearing technology (64) Donkey Anti-Rabbit (2) Flow Cytometry (1158)

Basic Description

Product Name Donkey Anti-Rabbit IgG H&L (AF647) - Secondary antibody, specific to , IgG
Synonyms Donkey Anti-Rabbit IgG H&L / AF647 | Immunoglobulin G | Donkey Anti-Rabbit IgG | Donkey Anti Rabbit IgG
Specifications & Purity ExactAb™, Validated, Azide Free, Ex:650nm, Em:668nm, 2.0mg/ml
Host species Donkey
Immunogen Native rabbit IgG
Positive Control IF/ICC: Mouse cerebellum; Mouse brain; Human brain; Rat cerebellum; Rat brain
Conjugation AF647
Excitation(nm) Red Laser(633nm)
Emission(nm) 668nm
Ex/Em(nm) Ex:650nm;Em:668nm
Grade Azide Free, Em:668nm, Ex:650nm, ExactAb™, Validated
Product Description

Donkey Anti-Rabbit IgG H&L (AF647), Secondary antibody, could be used for Flow, IF, ICC and so on.

Product Properties

Isotype IgG
Purification Method Protein G purified
Shape Liquid
Concentration 2.0mg/ml
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at -20℃ for one year. Avoid repeated freeze/thaw cycles

Images

Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882) - IF
IF analysis of HeLa cells labeling FXR1 with purified Recombinant FXR1 Antibody (Ab104281) at 1/1000 dilution. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were counterstained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution - Goat Anti-Mouse IgG H&L (AF488) 1/1000. Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882) was used as the secondary antibody at 1/1000 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882) - IHC
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum). Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min.Blocking buffer (normal goat serum) at 37°C for 30min. Antibody incubation with (GFAP) polyclonal antibody at 1/200 overnight at 4°C, followed by a conjugated Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882) for 90 minutes, and DAPI for nuclei staining.

Application

Application Dilution info
Flow Cytometry

1/100-1/1000

IF/ICC

1/100-1/1000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0800765 Certificate of Analysis Aug 03, 2023 Ab136882
ZJ23F0800766 Certificate of Analysis Aug 03, 2023 Ab136882

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Documents & Articles

Antibody structure and isotypes
Categories: Technical articles
Tags:
Immunoglobulin Antibody isotype Heavy chain and light chain
Flow Cytometry
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Flow Cytometry
Flow cytometry analysis
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Forward scatter Side scatter Dichroic filters/mirrors The multicolor flow cytometer
How to choose and use antibodies-primary antibody?
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Recombinant antibody Knockout gene ICC
How to find the right secondary antibody?
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Fluorophore Direct/Indirect Detection Enzyme markers
Flow cytometry protocol
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Antibodies Flow Cytometry Flow Antibodies Reactive dyes Indirect antibody labelling
Immunocytochemistry (ICC) protocol
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Antibody Biological buffer Fluorescent dyes Immunochemical staining Indirect antibody labeling
RNA dot blot protocol
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Protein Electrophoresis & Western Blotting Dot Blotting Primary antibodies Secondary Antibodies
In-cell ELISA protocol
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Monoclonal antibodies In-cell ELISA Cytoblot Protein quantification HRP-Conjugated Secondary Antibodies Polyclonal Antibodies
Caspase Immunofluorescence Staining Protocol
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Secondary antibody Apoptosis Programmed Death Immunofluorescence Caspase
General Protocol for Western Blotting
Categories: Protocols
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Western blotting WB Protein Electrophoresis & Western Blotting Biological buffers Immunoblotting Conjugated Secondary Antibodies HRP
Western Blotting with Fluorescently Labeled Antibodies
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Protein Electrophoresis & Western Blotting Biological buffers Immunoblotting Fluorescently Labeled Antibodies Membrane Transfer
Immunofluorescence Staining Protocol (Methanol permeabilization)
Categories: Protocols
Tags:
Frozen section Immunofluorescence staining Tissue section Methanol permeability IF
Guidelines of Secondary Antibody for Western Blotting
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Tags:
Secondary antibody WB Western blot HRP Horseradish peroxidase Secondary Antibody Selection Guide Alexa Fluor
Flow Cytometry Using Methanol Permeabilization
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Tags:
Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection
CLARITY Tissue Clearing Staining Technology
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CLARITY tissue clearing technology CLARITY tissue clearing lipid removal biological tissue 3D imaging Hydrogel
Fixation and Permeabilization Protocol for Transcription Factor Flow Cytometry
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Flow Cytometry Transcription Factors Transcription factor flow cytometry Cell fixation and permeabilization Cell fixation Cell permeabilization FOXP3

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