beta Actin Mouse mAb (TRITC)

COA

级别和纯度:

0.5 mg/mL

应用:

IF/ICC

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
亚型: Mouse IgG1
克隆号: FG01/2D10
偶联: TRITC

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货号 (SKU)	包装规格	是否现货
Ab176323-10μl	10μl	现货
Ab176323-20μl	20μl	现货
Ab176323-50μl	50μL	现货
Ab176323-100μl	100μL	现货
Ab176323-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: ACTB

Accession#:P60709 (9) ACTB (10) beta Actin (6) Gene ID:60 (10) Loading control (34) TRITC (16)

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基本信息

产品名称	beta Actin Mouse mAb (TRITC)
别名	β actin antibody \| β-actin antibody \| TRITC-conjugated β-Actin Mouse mAb \| 四甲基罗丹明偶联的抗-β-肌动蛋白抗体 \| β 肌动蛋白小鼠单克隆抗体 (TRITC) \| β 肌动蛋白小鼠单抗 (TRITC)
英文别名	A26C1A antibody \| A26C1B antibody \| ACTB antibody \| ACTB_HUMAN antibody \| Actin beta antibody \| Actin cytoplasmic 1 antibody \| Actin, cytoplasmic 1, N-terminally processed antibody \| Actx antibody \| b actin antibody \| b-actin antibody \| Beta cytoskeletal
规格或纯度	ExactAb™, 已验证, 无叠氮钠, Ex:557nm, Em:576nm, 0.5 mg/mL
宿主种属	小鼠(Mouse)
特异性	beta actin
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
预测反应种属	兔(Rabbit),牛(Cow),狗(Dog),猪(Pig),鸡(Chicken)
免疫原	Synthetic peptide in beta Actin
阳性对照	IF: Hela
偶联	TRITC
Excitation(nm)	Green Laser(532nm)
Emission(nm)	576nm
Ex/Em(nm)	Ex:557nm;Em:576nm

产品属性

克隆类型	单克隆抗体
克隆号	FG01/2D10
Format	Whole IgG
亚型	IgG1
轻链亚型	kappa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5 mg/mL
储存温度	避光,-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20℃ 下可保存一年。避免反复冻融循环。避免光照。
分子类型	抗体

关联靶点（人）

ACTB Tbio 肌动蛋白，细胞质 1(Actin, cytoplasmic 1) (0 活性数据)

点击查看靶蛋白信息： ACTB

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

IF: beta Actin Mouse mAb (TRITC) (Ab176323)

beta Actin Mouse mAb (TRITC) (Ab176323) - IF
IF analysis of beta Actin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (TRITC) (Ab176323) at 1/1000 dilution in blocking buffer overnight at 4°C. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

应用

应用名称	稀释比例
IF/ICC	1:1000

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C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到4个结果

批号(Lot Number)	证书类型	货号
ZJ24F0102295	分析证书	Ab176323
ZJ24F0102296	分析证书	Ab176323
ZJ24F0102297	分析证书	Ab176323
ZJ24F0102298	分析证书	Ab176323

技术文档和文章

免疫荧光染色实验步骤（甲醇通透法）

Categories: 实验方案（Protocols）

探索主题:

冷冻切片免疫荧光染色组织切片甲醇通透 IF

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: 实验方案（Protocols）

探索主题:

Frozen section IF Immunofluorescence staining Tissue section Methanol permeability

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: 实验方案（Protocols）

探索主题:

Frozen section IF Immunofluorescence staining Tissue section Methanol permeability

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引用文献

1. Wang RE, Hunt CR, Chen J, Taylor JS.. (2011) Biotinylated quercetin as an intrinsic photoaffinity proteomics probe for the identification of quercetin target proteins.. Bioorg Med Chem, 19 (16): (4710-4720). [PMID:21798748] [10.1016/j.bmc.2011.07.005]
2. Angelo LS, Maxwell DS, Wu JY, Sun D, Hawke DH, McCutcheon IE, Slopis JM, Peng Z, Bornmann WG, Kurzrock R.. (2013) Binding partners for curcumin in human schwannoma cells: biologic implications.. Bioorg Med Chem, 21 (4): (932-939). [PMID:23294827] [10.1016/j.bmc.2012.12.008]
3. Ohmori, H H, Toyama, S S and Toyama, S S.. (1992) Direct proof that the primary site of action of cytochalasin on cell motility processes is actin.. The Journal of cell biology, [PMID:1734024]
4. Nakajima-Iijima, S S, Hamada, H H, Reddy, P P and Kakunaga, T T.. (1985) Molecular structure of the human cytoplasmic beta-actin gene: interspecies homology of sequences in the introns.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2994062]
5. Ponte, P P, Ng, S Y SY, Engel, J J, Gunning, P P and Kedes, L L.. (1984) Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA.. Nucleic acids research, (10): [PMID:6322116]
6. Hanukoglu, I I, Tanese, N N and Fuchs, E E.. (1983) Complementary DNA sequence of a human cytoplasmic actin. Interspecies divergence of 3' non-coding regions.. Journal of molecular biology, (5): [PMID:6842590]
7. Zhang, Suisheng S and 5 more authors.. (2002) Nuclear DNA helicase II/RNA helicase A binds to filamentous actin.. The Journal of biological chemistry, (4): [PMID:11687588]
8. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801]
9. Stüven, Theis T, Hartmann, Enno E and Görlich, Dirk D.. (2003) Exportin 6: a novel nuclear export receptor that is specific for profilin.actin complexes.. The EMBO journal, (3): [PMID:14592989]
10. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843]
11. Holaska, James M JM, Kowalski, Amy K AK and Wilson, Katherine L KL.. (2004) Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane.. PLoS biology, [PMID:15328537]
12. Giannakopoulos, Nadia V NV and 9 more authors.. (2005) Proteomic identification of proteins conjugated to ISG15 in mouse and human cells.. Biochemical and biophysical research communications, (21): [PMID:16139798]
13. Procaccio, Vincent V and 14 more authors.. (2006) A mutation of beta -actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.. American journal of human genetics, [PMID:16685646]
14. Jønson, Lars L and 8 more authors.. (2007) Molecular composition of IMP1 ribonucleoprotein granules.. Molecular & cellular proteomics : MCP, [PMID:17289661]
15. Lu, Xiaoqing X and 5 more authors.. (2007) HGAL, a lymphoma prognostic biomarker, interacts with the cytoskeleton and mediates the effects of IL-6 on cell migration.. Blood, (15): [PMID:17823310]
16. Lange, Martin M and 16 more authors.. (2008) Regulation of muscle development by DPF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex.. Genes & development, (1): [PMID:18765789]
17. Kudryashov, Dmitri S DS and 10 more authors.. (2008) Connecting actin monomers by iso-peptide bond is a toxicity mechanism of the Vibrio cholerae MARTX toxin.. Proceedings of the National Academy of Sciences of the United States of America, (25): [PMID:19015515]
18. Mi, Lixin and 8 more authors.. (2011) Identification of potential protein targets of isothiocyanates by proteomics.. Chemical research in toxicology, (17): [PMID:21838287]
19. Schmidt, Mathias V MV and 19 more authors.. (2011) Tumor suppressor down-regulated in renal cell carcinoma 1 (DRR1) is a stress-induced actin bundling factor that modulates synaptic efficacy and cognition.. Proceedings of the National Academy of Sciences of the United States of America, (11): [PMID:21969592]
20. Rivière, Jean-Baptiste JB and 32 more authors.. (2012) De novo mutations in the actin genes ACTB and ACTG1 cause Baraitser-Winter syndrome.. Nature genetics, (26): [PMID:22366783]
21. Li, Ming-Ming MM and 18 more authors.. (2013) ALKBH4-dependent demethylation of actin regulates actomyosin dynamics.. Nature communications, [PMID:23673617]
22. Heisler, David B and 10 more authors.. (2015) ACTIN-DIRECTED TOXIN. ACD toxin-produced actin oligomers poison formin-controlled actin polymerization.. Science (New York, N.Y.), (31): [PMID:26228148]
23. Mu, P P, Akashi, T T, Lu, F F, Kishida, S S and Kadomatsu, K K.. (2017) A novel nuclear complex of DRR1, F-actin and COMMD1 involved in NF-κB degradation and cell growth suppression in neuroblastoma.. Oncogene, (12): [PMID:28604741]
24. Drazic, Adrian A and 12 more authors.. (2018) NAA80 is actin's N-terminal acetyltransferase and regulates cytoskeleton assembly and cell motility.. Proceedings of the National Academy of Sciences of the United States of America, (24): [PMID:29581253]
25. Schrank, Benjamin R BR and 7 more authors.. (2018) Nuclear ARP2/3 drives DNA break clustering for homology-directed repair.. Nature, [PMID:29925947]
26. Wiame, Elsa E and 6 more authors.. (2018) NAT6 acetylates the N-terminus of different forms of actin.. The FEBS journal, [PMID:30028079]
27. Kwiatkowski, Sebastian S and 10 more authors.. (2018) SETD3 protein is the actin-specific histidine N-methyltransferase.. eLife, (11): [PMID:30526847]
28. Wilkinson, Alex W AW and 21 more authors.. (2019) SETD3 is an actin histidine methyltransferase that prevents primary dystocia.. Nature, [PMID:30626964]
29. Guo, Qiong Q and 9 more authors.. (2019) Structural insights into SETD3-mediated histidine methylation on β-actin.. eLife, (20): [PMID:30785395]

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beta Actin Mouse mAb (TRITC)

库存信息

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基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

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引用文献

溶液计算器

摩尔浓度计算器

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