| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab091004-10μl |
10μl |
现货  |
| |
| Ab091004-50μl |
50μL |
现货 |
|
| 产品名称 | beta Actin Antibody |
|---|---|
| 别名 | β-肌动蛋白抗体 | ACTB抗体 | Anti-beta Actin抗体 | β-Actin | beta 肌动蛋白抗体 |
| 英文别名 | A26C1A antibody | A26C1B antibody | ACTB antibody | ACTB_HUMAN antibody | Actin beta antibody | Actin cytoplasmic 1 antibody | Actin, cytoplasmic 1, N-terminally processed antibody | Actx antibody | b actin antibody | b-actin antibody | Beta cytoskeletal |
| 规格或纯度 | ExactAb™, Validated, Azide Free, High performance, 1.0mg/mL |
| 宿主种属 | 兔(Rabbit) |
| 特异性 | ACTB |
| 应用 | WB,Flow,IF,ICC,IHC |
| 种属反应性 | 人(Human),大鼠(Rat),小鼠(Mouse) |
| 预测反应种属 | 仓鼠(Hamster),兔(Rabbit),狗(Dog),猪(Pig),猫(Cat),绵羊(Sheep),蜜蜂(Bee),豚鼠(Guinea pig),鱼(Fish),鸡(Chicken) |
| 免疫原 | Synthetic MAP peptide derived from human beta-Actin: 1-200/375. |
| 阳性对照 | WB: NIH/3T3, C6, A549, Ramos, Jurkat, HeLa, MCF-7 lysates. IHC: Human cervical carcinoma tissue. ICC/IF: HeLa cells. |
| 偶联 | Unconjugated |
| 备注 | 本产品即将售完下架，替代产品为 Ab090999 |
| 抗体类型 | Primary antibody |
|---|---|
| 克隆类型 | 多克隆抗体 |
| Format | Whole IgG |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 来源 | Serum |
| 物理外观 | Liquid |
| 储存缓冲液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% ProClin 300 and 50% Glycerol. |
| 防腐剂 | 0.03% ProClin 300 |
| 浓度 | 1.0mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 在 -20 °C 下保存一年。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
beta Actin Antibody (Ab091004) - Western Blot
All lanes: beta Actin Antibody (Ab091004) at 1/50000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
beta Actin Antibody (Ab091004) - IHC
Tissue: Human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded.
Antigen retrieval: Citrate buffer (0.01M, pH 6.0) boiling bathing for 15 minutes; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37℃ for 20 minutes.
Incubation: beta Actin Antibody (Ab091004) 1/1500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
beta Actin Antibody (Ab091004) - ICC/IF
Hela cells: 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with beta Actin Antibody (Ab091004) 1/100, 90 minutes at 37°C; Followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37°C for 90 minutes, DAPI was used to stain the cell nuclei.
| 应用名称 | 稀释比例 |
|---|---|
| WB |
1:5000-50000
|
| IHC |
1:500-1000
|
| IF/ICC |
1:100
|
| Flow Cytometry | ≤1.0μg/test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. |
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1215526 |
分析证书 | Ab091004 |
| ZJ23F0700611 |
分析证书 | Ab091004 |
| ZJ23F0700610 |
分析证书 | Ab091004 |
| ZJ24F0404301 |
分析证书 | Ab091004 |
| ZJ24F1011819 |
分析证书 | Ab091004 |
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