YAP1 Mouse mAb

COA

级别和纯度:

无载体

已验证

ExactAb™

1.0 mg/mL

应用:

ELISA
Flow Cytometry
IHC
WB

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG1
克隆号: 1A12
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab134985-10μl	10μl	现货
Ab134985-50μl	50μL	现货
Ab134985-100μl	100μL	现货
Ab134985-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: YAP1

Accession#:P46937 (1) Gene ID:10413 (1) YAP1 (1) 流式细胞术 (1163)

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基本信息

产品名称	YAP1 Mouse mAb
别名	YAP1 小鼠单克隆抗体 \| YAP1 鼠单克隆抗体 \| 小鼠 YAP1单克隆抗体 \| 鼠 YAP1 单克隆抗体 \| YAP1 小鼠抗体 \| YAP1 鼠抗体 \| 小鼠 YAP1 抗体 \| 鼠 YAP1 抗体 \| 鼠抗人 YAP1 单克隆抗体 \| 小鼠抗人 YAP1 单克隆抗体 \| 鼠抗人 YAP1 抗体 \| 小鼠抗人 YAP1 抗体 \| YAP1小鼠mAb
英文别名	65 kDa Yes-associated protein \| COB1 \| Protein Yorkie Homolog \| Transcriptional Coactivator YAP1 \| YAP \| YAP1 \| YAP1-2gamma \| YAP2 \| YAP2L \| YAP65 \| YAP65yes-associated protein 2 \| Yes Associated Protein \| Yes-associated protein 1 \| Yes-associated protein
规格或纯度	ExactAb™, Validated, Carrier Free, 1.0 mg/mL
宿主种属	小鼠(Mouse)
特异性	YAP1
种属反应性	人(Human)
免疫原	Purified recombinant fragment of human YAP1 (AA: 250-447) expressed in E. Coli.
阳性对照	WB: HeLa, MCF7, A549 and C6 lysates. IHC: Prostate cancer tissue, Rectum cancer tissue. Flow: HeLa cells.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	1A12
Format	Whole IgG
亚型	IgG1
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein G purified
物理外观	Liquid
储存缓冲液	Purified antibody in 10mM PBS with 0.05% sodium azide, pH7.4.
防腐剂	0.05% Sodium Azide
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	交付后，等分并储存于 -20°C。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

YAP1 Tchem 转录辅激活因子 YAP1(Transcriptional coactivator YAP1) (0 活性数据)

点击查看靶蛋白信息： YAP1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

YAP1 Tchem Transcriptional coactivator YAP1 (194 活性数据)

点击查看靶蛋白信息： YAP1

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: YAP1 Mouse mAb (Ab134985)

YAP1 Mouse mAb (Ab134985) - Western Blot
All lanes: YAP1 Mouse mAb (Ab134985) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution

Predicted band size: 65 kDa
Observed band size: 65 kDa

IHC: YAP1 Mouse mAb (Ab134985)

YAP1 Mouse mAb (Ab134985) - IHC
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues using YAP1 Mouse mAb (Ab134985) with DAB staining.

IHC: YAP1 Mouse mAb (Ab134985)

YAP1 Mouse mAb (Ab134985) - IHC
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using YAP1 Mouse mAb (Ab134985) with DAB staining.

Flow Cytometry: YAP1 Mouse mAb (Ab134985)

YAP1 Mouse mAb (Ab134985) - Flow Cytometry
Flow cytometric analysis of HeLa cells using YAP1 Mouse mAb (Ab134985) (green) and negative control (red).

应用

应用名称	稀释比例
WB	1/500-1/2000
IHC	1/200-1/1000
ELISA	1/10000
Flow Cytometry	1/200-1/400

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0521475	分析证书	Ab134985
ZJ24F0404294	分析证书	Ab134985
ZJ23F0700719	分析证书	Ab134985

技术文档和文章

流式细胞术

Categories: 技术文章

探索主题:

流式细胞术

流式细胞仪分析

Categories: 技术文章

探索主题:

前向散射侧向散射二向色滤光片多色流式细胞仪

流式细胞术实验方案

Categories: 实验方案（Protocols）

探索主题:

抗体流式细胞术流式抗体活性染料间接抗体标记

采用碘化丙啶DNA染色法进行流式细胞术细胞周期分析

Categories: 实验方案（Protocols）

探索主题:

流式抗体溴化丙锭细胞周期

石蜡样品的免疫组化染色方案（IHC-P）

Categories: 实验方案（Protocols）

探索主题:

单克隆抗体石蜡切片免疫组化染色

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）

Categories: 实验方案（Protocols）

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重组抗体单克隆抗体 IHC 免疫组化冷冻切片

免疫组织化学染色的抗原修复方案

Categories: 实验方案（Protocols）

探索主题:

重组抗体单克隆抗体 IHC 免疫组化抗原修复

利用甲醇通透法进行流式细胞术

Categories: 实验方案（Protocols）

探索主题:

流式细胞术流式抗体流式实验甲醇通透细胞分选细胞分型免疫研究细胞内抗原检测

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

引用文献

1. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811]
2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
3. Jongshin Kim,Haiin Jo,Hyowon Hong,Min Hwan Kim,Jin Man Kim,June-Koo Lee,Won Do Heo,Joon Kim. (2015-04-08) Actin remodelling factors control ciliogenesis by regulating YAP/TAZ activity and vesicle trafficking.. Nature communications, 6 (6781-6781). [PMID:25849865]
4. Zhang Z, Lin Z, Zhou Z, Shen HC, Yan SF, Mayweg AV, Xu Z, Qin N, Wong JC, Zhang Z, Rong Y, Fry DC, Hu T.. (2014) Structure-Based Design and Synthesis of Potent Cyclic Peptides Inhibiting the YAP-TEAD Protein-Protein Interaction.. ACS Med Chem Lett, 5 (9): (993-998). [PMID:25221655] [10.1021/ml500160m]
5. Sudol, M M and 7 more authors.. (1995) Characterization of the mammalian YAP (Yes-associated protein) gene and its role in defining a novel protein module, the WW domain.. The Journal of biological chemistry, (16): [PMID:7782338]
6. Chen, H I HI and 7 more authors.. (1997) Characterization of the WW domain of human yes-associated protein and its polyproline-containing ligands.. The Journal of biological chemistry, (4): [PMID:9202023]
7. Pires, J R JR and 8 more authors.. (2001) Solution structures of the YAP65 WW domain and the variant L30 K in complex with the peptides GTPPPPYTVG, N-(n-octyl)-GPPPY and PLPPY and the application of peptide libraries reveal a minimal binding epitope.. Journal of molecular biology, (14): [PMID:11743730]
8. Komuro, Akihiko A, Nagai, Makoto M, Navin, Nicholas E NE and Sudol, Marius M.. (2003) WW domain-containing protein YAP associates with ErbB-4 and acts as a co-transcriptional activator for the carboxyl-terminal fragment of ErbB-4 that translocates to the nucleus.. The Journal of biological chemistry, (29): [PMID:12807903]
9. Hoshino, Masataka M and 17 more authors.. (2006) Transcriptional repression induces a slowly progressive atypical neuronal death associated with changes of YAP isoforms and p73.. The Journal of cell biology, (13): [PMID:16461361]
10. Kulman, John D JD, Harris, Jeff E JE, Xie, Ling L and Davie, Earl W EW.. (2007) Proline-rich Gla protein 2 is a cell-surface vitamin K-dependent protein that binds to the transcriptional coactivator Yes-associated protein.. Proceedings of the National Academy of Sciences of the United States of America, (22): [PMID:17502622]
11. Matsuoka, Shuhei S and 12 more authors.. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.. Science (New York, N.Y.), (25): [PMID:17525332]
12. Paliwal, Preeti P, Radha, Vegesna V and Swarup, Ghanshyam G.. (2007) Regulation of p73 by Hck through kinase-dependent and independent mechanisms.. BMC molecular biology, (30): [PMID:17535448]
13. Zhao, Bin B and 15 more authors.. (2007) Inactivation of YAP oncoprotein by the Hippo pathway is involved in cell contact inhibition and tissue growth control.. Genes & development, (1): [PMID:17974916]
14. Hao, Yawei Y, Chun, Alex A, Cheung, Kevin K, Rashidi, Babak B and Yang, Xiaolong X.. (2008) Tumor suppressor LATS1 is a negative regulator of oncogene YAP.. The Journal of biological chemistry, (29): [PMID:18158288]
15. Levy, Dan D, Adamovich, Yaarit Y, Reuven, Nina N and Shaul, Yosef Y.. (2008) Yap1 phosphorylation by c-Abl is a critical step in selective activation of proapoptotic genes in response to DNA damage.. Molecular cell, (15): [PMID:18280240]
16. Zhao, Bin B and 11 more authors.. (2008) TEAD mediates YAP-dependent gene induction and growth control.. Genes & development, (15): [PMID:18579750]
17. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
18. Zhao, Bin B, Li, Li L, Tumaneng, Karen K, Wang, Cun-Yu CY and Guan, Kun-Liang KL.. (2010) A coordinated phosphorylation by Lats and CK1 regulates YAP stability through SCF(beta-TRCP).. Genes & development, (1): [PMID:20048001]
19. Li, Ze Z and 7 more authors.. (2010) Structural insights into the YAP and TEAD complex.. Genes & development, (1): [PMID:20123905]
20. Tomlinson, V V and 5 more authors.. (2010) JNK phosphorylates Yes-associated protein (YAP) to regulate apoptosis.. Cell death & disease, [PMID:21364637]
21. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
22. Liu, X X and 6 more authors.. (2013) PTPN14 interacts with and negatively regulates the oncogenic function of YAP.. Oncogene, (7): [PMID:22525271]
23. Gaffney, Christian J CJ and 11 more authors.. (2012) Identification, basic characterization and evolutionary analysis of differentially spliced mRNA isoforms of human YAP1 gene.. Gene, (10): [PMID:22939869]
24. Yazicioglu, Mustafa N MN and 7 more authors.. (2013) Cellular localization and characterization of cytosolic binding partners for Gla domain-containing proteins PRRG4 and PRRG2.. The Journal of biological chemistry, (6): [PMID:23873930]
25. Williamson, Kathleen A KA and 16 more authors.. (2014) Heterozygous loss-of-function mutations in YAP1 cause both isolated and syndromic optic fissure closure defects.. American journal of human genetics, (6): [PMID:24462371]
26. Liu, Jie J and 13 more authors.. (2017) Loss of DLG5 promotes breast cancer malignancy by inhibiting the Hippo signaling pathway.. Scientific reports, (7): [PMID:28169360]
27. Zhou, Beiyun B and 22 more authors.. (2018) Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis.. The Journal of clinical investigation, (1): [PMID:29400695]
28. Amisaki, Masataka M, Tsuchiya, Hiroyuki H, Sakabe, Tomohiko T, Fujiwara, Yoshiyuki Y and Shiota, Goshi G.. (2019) Identification of genes involved in the regulation of TERT in hepatocellular carcinoma.. Cancer science, [PMID:30447097]

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体积（添加到小瓶中）

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所需的复溶浓度

YAP1 Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器