货号 (SKU)

包装规格

是否现货

价格

数量

Ab131081-10μl

10μl

现货

Ab131081-50μl

50μL

现货

Ab131081-100μl

100μL

期货

Ab131081-1ml

1ml

期货

基本信息

产品名称	Thymidine Kinase 1/TK1 Mouse mAb
别名	细胞质胸苷激酶1单克隆抗体 \| 胸苷激酶 1/TK1 小鼠 mAb
英文别名	cytosolic antibody \| KITH_HUMAN antibody \| Thymidine kinase 1 antibody \| Thymidine kinase 1 soluble antibody \| Thymidine kinase 1 soluble isoform antibody \| Thymidine kinase antibody \| Thymidine kinase cytosolic antibody \| TK 1 antibody \| TK 2 antibody \|
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属	小鼠(Mouse)
特异性	Thymidine Kinase 1/TK1
种属反应性	人(Human),猪(Pig)
免疫原	Recombinant TK1 expressed in E.coli (AA 2-234).
阳性对照	WB: HeLa, MCF7, MOLT-4 and A549 cell lysates. ICC/IF: MCF7 cells.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	C3
Format	Whole IgG
亚型	Mouse IgG2b
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified + Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂	0.05% Proclin 300
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

TK1 Tchem 胸苷激酶，细胞质(Thymidine kinase, cytosolic) (0 活性数据)

点击查看靶蛋白信息： TK1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: Thymidine Kinase 1/TK1 Mouse mAb (Ab131081)

Thymidine Kinase 1/TK1 Mouse mAb (Ab131081) - Western Blot
All lanes: Thymidine Kinase 1/TK1 Mouse mAb (Ab131081) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 26 kDa
Exposure time: 60.0 s

IF/ICC: Thymidine Kinase 1/TK1 Mouse mAb (Ab131081)

Thymidine Kinase 1/TK1 Mouse mAb (Ab131081) - IF/ICC
FITC staining on IF
Samples: Human MCF7 cells
Primary Ab: 20 µg/mL Thymidine Kinase 1/TK1 Mouse mAb (Ab131081)
Second Ab: 1 µg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

应用

应用名称	稀释比例
WB	0.01-6 µg/mL
IHC	5-50µg/mL

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到5个结果

批号(Lot Number)	证书类型	货号
ZJ23F1202139	分析证书	Ab131081
ZJ23F1202140	分析证书	Ab131081
ZJ23F1202141	分析证书	Ab131081
ZJ24F1011961	分析证书	Ab131081
ZJ24F1011958	分析证书	Ab131081

技术文档和文章

石蜡样品的免疫组化染色方案（IHC-P）

Categories: 实验方案（Protocols）

探索主题:

单克隆抗体石蜡切片免疫组化染色

叠氮化钠去除实验

Categories: 实验方案（Protocols）

探索主题:

重组抗体叠氮化钠去除抗体纯化葡聚糖凝胶G-25 无叠氮化钠抗体

Sodium Azide Removal Protocol

Categories: 实验方案（Protocols）

探索主题:

Recombinant antibody Sodium Azide Removal Antibody Purification Sephadex G-25 Sodium Azide-Free Antibody

引用文献

1. Bobek M, Kavai I, Sharma RA, Grill S, Dutschman G, Cheng YC.. (1987) Acetylenic nucleosides. 4. 1-beta-D-arabinofuranosyl-5-ethynylcytosine. Improved synthesis and evaluation of biochemical and antiviral properties.. J Med Chem, 30 (11): (2154-2157). [PMID:2822932] [10.1021/jm00394a039]
2. Haines DR, Tseng CK, Marquez VE.. (1987) Synthesis and biological activity of unsaturated carboacyclic purine nucleoside analogues.. J Med Chem, 30 (5): (943-947). [PMID:3033247] [10.1021/jm00388a036]
3. Spadari S, Maga G, Focher F, Ciarrocchi G, Manservigi R, Arcamone F, Capobianco M, Carcuro A, Colonna F, Iotti S.. (1992) L-thymidine is phosphorylated by herpes simplex virus type 1 thymidine kinase and inhibits viral growth.. J Med Chem, 35 (22): (4214-4220). [PMID:1331461] [10.1021/jm00100a029]
4. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940]
5. Flemington, E E, Bradshaw, H D HD, Traina-Dorge, V V, Slagel, V V and Deininger, P L PL.. (1987) Sequence, structure and promoter characterization of the human thymidine kinase gene.. Gene, [PMID:3301530]
6. Kreidberg, J A JA and Kelly, T J TJ.. (1986) Genetic analysis of the human thymidine kinase gene promoter.. Molecular and cellular biology, [PMID:3785218]
7. Bradshaw, H D HD and Deininger, P L PL.. (1984) Human thymidine kinase gene: molecular cloning and nucleotide sequence of a cDNA expressible in mammalian cells.. Molecular and cellular biology, [PMID:6549046]
8. Chang, Z F ZF, Huang, D Y DY and Chi, L M LM.. (1998) Serine 13 is the site of mitotic phosphorylation of human thymidine kinase.. The Journal of biological chemistry, (15): [PMID:9575153]
9. Li, Chia-Lung CL, Lu, Ching-Yi CY, Ke, Po-Yuan PY and Chang, Zee-Fen ZF.. (2004) Perturbation of ATP-induced tetramerization of human cytosolic thymidine kinase by substitution of serine-13 with aspartic acid at the mitotic phosphorylation site.. Biochemical and biophysical research communications, (16): [PMID:14697231]
10. Ke, Po-Yuan PY and Chang, Zee-Fen ZF.. (2004) Mitotic degradation of human thymidine kinase 1 is dependent on the anaphase-promoting complex/cyclosome-CDH1-mediated pathway.. Molecular and cellular biology, [PMID:14701726]
11. Welin, Martin M and 8 more authors.. (2004) Structures of thymidine kinase 1 of human and mycoplasmic origin.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:15611477]
12. Birringer, Markus S MS and 5 more authors.. (2005) Structure of a type II thymidine kinase with bound dTTP.. FEBS letters, (28): [PMID:15733844]
13. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243]
14. Segura-Peña, Dario D, Lutz, Stefan S, Monnerjahn, Christian C, Konrad, Manfred M and Lavie, Arnon A.. (2007) Binding of ATP to TK1-like enzymes is associated with a conformational change in the quaternary structure.. Journal of molecular biology, (25): [PMID:17407781]
15. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895]
16. Skovgaard, Tine T, Uhlin, Ulla U and Munch-Petersen, Birgitte B.. (2012) Comparative active-site mutation study of human and Caenorhabditis elegans thymidine kinase 1.. The FEBS journal, [PMID:22385435]
17. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

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g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

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复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

Thymidine Kinase 1/TK1 Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器

Thymidine Kinase 1/TK1 Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器