This is a demo store. No orders will be fulfilled.

HLA Class II DRB1 Mouse mAb

    应用:
  • WB
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human)
  • 亚型: Mouse IgG2b
  • 克隆号: C1
  • 偶联: Unconjugated
有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭
货号 (SKU) 包装规格 是否现货 价格 数量
Ab108170-10μl
10μl 现货 Stock Image
Ab108170-50μl
50μL 现货 Stock Image
Ab108170-100μl
100μL 期货 Stock Image
Ab108170-1ml
1ml 期货 Stock Image

基本信息

产品名称 HLA Class II DRB1 Mouse mAb
别名 HLA Class II DRB1抗体 | Ⅱ类主要组织相容性复合体DRβ1抗体 | HLA II 类 DRB1 小鼠 mAb
英文别名 HLA-DRB1 | Human leukocyte antigen DRB1 | 2B1F_HUMAN antibody | DR1 antibody | DR16 antibody | DR5 antibody | DR8 antibody | DRB1 antibody | DRw10 antibody | DW2.2/DR2.2 antibody | FLJ75017 antibody | FLJ76359 antibody | HLA class II antigen beta chain an
规格或纯度 无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属 小鼠(Mouse)
特异性 HLA Class II DRB1
种属反应性 人(Human)
免疫原 Recombinant HLA-DRB1 expressed in E.coli (AA 30-266)
阳性对照 WB: Raji and Ramos cell lysates.
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 C1
Format Whole IgG
亚型 Mouse IgG2b
轻链亚型 kappa
SDS-PAGE 150 kDa
纯化方法 Protein A purified + Protein G purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂 0.05% Proclin 300
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

HLA-DRB1 Tbio HLA II 类组织相容性抗原,DRB1-9 β 链(HLA class II histocompatibility antigen, DRB1-9 beta chain) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
HLA-DRB1 Tbio HLA II 类组织相容性抗原,DRB1-4 β 链(HLA class II histocompatibility antigen, DRB1-4 beta chain) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
HLA-DRB1 Tbio HLA II 类组织相容性抗原,DRB1-16 β 链(HLA class II histocompatibility antigen, DRB1-16 beta chain) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
HLA-DRB1 Tbio HLA II 类组织相容性抗原,DRB1-3 链(HLA class II histocompatibility antigen, DRB1-3 chain) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
HLA-DRB1 Tbio HLA II 类组织相容性抗原,DRB1-12 β 链(HLA class II histocompatibility antigen, DRB1-12 beta chain) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
HLA-DRB1 Tbio HLA II 类组织相容性抗原,DRB1-13 β 链(HLA class II histocompatibility antigen, DRB1-13 beta chain) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
HLA-DRB1 Tbio HLA II 类组织相容性抗原,DRB1-8 β 链(HLA class II histocompatibility antigen, DRB1-8 beta chain) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
HLA-DRB1 Tbio HLA II 类组织相容性抗原,DRB1-11 β 链(HLA class II histocompatibility antigen, DRB1-11 beta chain) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
HLA-DRB1 Tchem HLA II 类组织相容性抗原,DRB1-15 β 链(HLA class II histocompatibility antigen, DRB1-15 beta chain) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
HLA-DRB1 Tbio HLA II 类组织相容性抗原,DRB1-1 β 链(HLA class II histocompatibility antigen, DRB1-1 beta chain) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
HLA-DRB1 Tbio HLA II 类组织相容性抗原,DRB1-14 β 链(HLA class II histocompatibility antigen, DRB1-14 beta chain) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
HLA-DRB1 Tbio HLA II 类组织相容性抗原,DRB1-7 β 链(HLA class II histocompatibility antigen, DRB1-7 beta chain) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)
HLA-DRB1 Tbio HLA II 类组织相容性抗原,DRB1-10 β 链(HLA class II histocompatibility antigen, DRB1-10 beta chain) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

图片

HLA Class II DRB1 Mouse mAb (Ab108170) - Western Blot
All lanes: HLA Class II DRB1 Mouse mAb (Ab108170) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 12.1 s

HLA Class II DRB1 Mouse mAb (Ab108170) - Western Blot
All lanes: HLA Class II DRB1 Mouse mAb (Ab108170) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 49.9 s

应用

应用名称 稀释比例
WB

0.01-2 µg/mL

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到3个结果

批号(Lot Number) 证书类型 货号
ZJ24F0404380 分析证书 Ab108170
ZJ24F0404379 分析证书 Ab108170
ZJ24F0404378 分析证书 Ab108170

技术文档和文章

蛋白质印迹的一抗选择指南
Guidelines of Primary Antibodies for Western Blotting
Guidelines of Primary Antibodies for Western Blotting
高分子量蛋白质印迹法
Protocol of Western Blotting For High Molecular Weights
Protocol of Western Blotting For High Molecular Weights
Protocol of Western Blotting For High Molecular Weights
Protocol of Western Blotting For High Molecular Weights
叠氮化钠去除实验
Sodium Azide Removal Protocol

引用文献

1. International Multiple Sclerosis Genetics Consortium, Wellcome Trust Case Control Consortium 2, Sawcer S, Hellenthal G, Pirinen M, Spencer CC, Patsopoulos NA, Moutsianas L, Dilthey A, Su Z et al..  (2011)  Genetic risk and a primary role for cell-mediated immune mechanisms in multiple sclerosis..  Nature,  476  (7359): (214-9).  [PMID:21833088]
2. Stephen W Scally,Jan Petersen,Soi Cheng Law,Nadine L Dudek,Hendrik J Nel,Khai Lee Loh,Lakshmi C Wijeyewickrema,Sidonia B G Eckle,Jurgen van Heemst,Robert N Pike,James McCluskey,Rene E Toes,Nicole L La Gruta,Anthony W Purcell,Hugh H Reid,Ranjeny Thomas,Jamie Rossjohn.  (2013-11-06)  A molecular basis for the association of the HLA-DRB1 locus, citrullination, and rheumatoid arthritis..  The Journal of experimental medicine,  210  ((12)): (2569-2582).  [PMID:24190431]
3. AeRyon Kim,Isamu Z Hartman,Brad Poore,Tatiana Boronina,Robert N Cole,Nianbin Song,M Teresa Ciudad,Rachel R Caspi,Dolores Jaraquemada,Scheherazade Sadegh-Nasseri.  (2014-11-22)  Divergent paths for the selection of immunodominant epitopes from distinct antigenic sources..  Nature communications,  (5369-5369).  [PMID:25413013]
4. Tapeinou A, Giannopoulou E, Simal C, Hansen BE, Kalofonos H, Apostolopoulos V, Vlamis-Gardikas A, Tselios T..  (2018)  Design, synthesis and evaluation of an anthraquinone derivative conjugated to myelin basic protein immunodominant (MBP85-99) epitope: Towards selective immunosuppression..  Eur J Med Chem,  143  (621-631).  [PMID:29216561] [10.1016/j.ejmech.2017.11.063]
5. Jonsson, A K AK, Andersson, L L and Rask, L L..  (1989)  A cellular and functional split in the DRw8 haplotype is due to a single amino acid replacement (DR beta ser 57- asp 57)..  Immunogenetics,  [PMID:2497068]
6. Lee, B S BS, Rust, N A NA, McMichael, A J AJ and McDevitt, H O HO..  (1987)  HLA-DR2 subtypes form an additional supertypic family of DR beta alleles..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:2885840]
7. Tonnelle, C C, DeMars, R R and Long, E O EO..  (1985)  DO beta: a new beta chain gene in HLA-D with a distinct regulation of expression..  The EMBO journal,  [PMID:2998758]
8. Young, J A JA, Wilkinson, D D, Bodmer, W F WF and Trowsdale, J J..  (1987)  Sequence and evolution of HLA-DR7- and -DRw53-associated beta-chain genes..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:3110774]
9. Liu, C P CP, Bach, F H FH and Wu, S K SK..  (1988)  Molecular studies of a rare DR2/LD-5a/DQw3 HLA class II haplotype. Multiple genetic mechanisms in the generation of polymorphic HLA class II genes..  Journal of immunology (Baltimore, Md. : 1950),  (15): [PMID:3129499]
10. Lock, C B CB, So, A K AK, Welsh, K I KI, Parkes, J D JD and Trowsdale, J J..  (1988)  MHC class II sequences of an HLA-DR2 narcoleptic..  Immunogenetics,  [PMID:3259543]
11. Tieber, V L VL, Abruzzini, L F LF, Didier, D K DK, Schwartz, B D BD and Rotwein, P P..  (1986)  Complete characterization and sequence of an HLA class II DR beta chain cDNA from the DR5 haplotype..  The Journal of biological chemistry,  (25): [PMID:3456344]
12. Wu, S K SK, Yabe, T T, Madden, M M, Saunders, T L TL and Bach, F H FH..  (1987)  cDNA cloning and sequencing reveals that the electrophoretically constant DR beta 2 molecules, as well as the variable DR beta 1 molecules, from HLA-DR2 subtypes have different amino acid sequences including a hypervariable region for a functionally important epitope..  Journal of immunology (Baltimore, Md. : 1950),  (1): [PMID:3571980]
13. Cairns, J S JS and 5 more authors..  (1985)  Sequence polymorphism of HLA DR beta 1 alleles relating to T-cell-recognized determinants..  Nature,  [PMID:3875800]
14. Gustafsson, K K and 8 more authors..  (1984)  Mutations and selection in the generation of class II histocompatibility antigen polymorphism..  The EMBO journal,  [PMID:6589154]
15. Walker, L E LE and 5 more authors..  (1983)  N-terminal amino acid sequences of the alpha and beta chains of HLA-DR1 and HLA-DR2 antigens..  Biochemistry,  (4): [PMID:6600932]
16. Kratzin, H H and 9 more authors..  (1981)  [Primary structure of class II human histocompatibility antigens. 1st communication. Amino acid sequence of the N-terminal 198 residues of the beta chain of a HLA-Dw2,2;DR2,2-alloantigen (author's transl)]..  Hoppe-Seyler's Zeitschrift fur physiologische Chemie,  [PMID:6947956]
17. Ghosh, P P, Amaya, M M, Mellins, E E and Wiley, D C DC..  (1995)  The structure of an intermediate in class II MHC maturation: CLIP bound to HLA-DR3..  Nature,  (30): [PMID:7477400]
18. Stern, L J LJ and 6 more authors..  (1994)  Crystal structure of the human class II MHC protein HLA-DR1 complexed with an influenza virus peptide..  Nature,  (17): [PMID:8145819]
19. Jardetzky, T S TS and 8 more authors..  (1994)  Three-dimensional structure of a human class II histocompatibility molecule complexed with superantigen..  Nature,  (21): [PMID:8152483]
20. Topalian, S L SL and 7 more authors..  (1996)  Melanoma-specific CD4+ T cells recognize nonmutated HLA-DR-restricted tyrosinase epitopes..  The Journal of experimental medicine,  (1): [PMID:8642306]
21. Li, Q Q and 6 more authors..  (1997)  Epstein-Barr virus uses HLA class II as a cofactor for infection of B lymphocytes..  Journal of virology,  [PMID:9151859]
22. Dessen, A A, Lawrence, C M CM, Cupo, S S, Zaller, D M DM and Wiley, D C DC..  (1997)  X-ray crystal structure of HLA-DR4 (DRA*0101, DRB1*0401) complexed with a peptide from human collagen II..  Immunity,  [PMID:9354468]
23. Martínez-Quiles, N N and 7 more authors..  (1998)  Complete cDNA sequence of the HLA-DRB1*09012 allele..  European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics,  [PMID:9777332]
24. Smith, K J KJ, Pyrdol, J J, Gauthier, L L, Wiley, D C DC and Wucherpfennig, K W KW..  (1998)  Crystal structure of HLA-DR2 (DRA*0101, DRB1*1501) complexed with a peptide from human myelin basic protein..  The Journal of experimental medicine,  (19): [PMID:9782128]
25. Doebele, R C RC, Busch, R R, Scott, H M HM, Pashine, A A and Mellins, E D ED..  (2000)  Determination of the HLA-DM interaction site on HLA-DR molecules..  Immunity,  [PMID:11070170]
26. Petersson, K K and 6 more authors..  (2001)  Crystal structure of a superantigen bound to MHC class II displays zinc and peptide dependence..  The EMBO journal,  (2): [PMID:11432818]
27. Chen, X X, Ji, Z L ZL and Chen, Y Z YZ..  (2002)  TTD: Therapeutic Target Database..  Nucleic acids research,  (1): [PMID:11752352]
28. Corell, A A and 5 more authors..  (2002)  Complete cDNA sequences of the HLA-DRB1*14011, *1402, *1403 and *1404 alleles..  Tissue antigens,  [PMID:11972886]
29. Ramon, D D and 5 more authors..  (2002)  Complete cDNA sequences of the HLA-DRB1*0402 and DRB1*11041 alleles..  European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics,  [PMID:12358860]
30. Kohsaka, Hitoshi H, Nasu, Kimio K, Matsushita, Sho S and Miyasaka, Nobuyuki N..  (2002)  Complete cDNA coding sequence of the HLA-DRB1*1405 allele..  DNA sequence : the journal of DNA sequencing and mapping,  [PMID:12652907]
31. Rossman, Milton D MD and 11 more authors..  (2003)  HLA-DRB1*1101: a significant risk factor for sarcoidosis in blacks and whites..  American journal of human genetics,  [PMID:14508706]
32. Arnon, Ruth R and Aharoni, Rina R..  (2004)  Mechanism of action of glatiramer acetate in multiple sclerosis and its potential for the development of new applications..  Proceedings of the National Academy of Sciences of the United States of America,  (5): [PMID:15371592]
33. Vilches, C C and 8 more authors..  (2005)  Complete coding sequences and haplotypic associations of HLA-B*0707, -B*1524, -B*4405, -B*4802, -DRB1*0409, -DRB1*0411, -DRB1*1115, -DRB1*1305, and the novel allele -DRB1*0709. Group-specific amplification of cDNA from DRB1 alleles associated to DRB3 and DRB4..  Tissue antigens,  [PMID:15896200]
34. Raymond, Christopher K CK and 10 more authors..  (2005)  Ancient haplotypes of the HLA Class II region..  Genome research,  [PMID:16140993]
35. Schulze zur Wiesch, Julian J and 16 more authors..  (2005)  Broad repertoire of the CD4+ Th cell response in spontaneously controlled hepatitis C virus infection includes dominant and highly promiscuous epitopes..  Journal of immunology (Baltimore, Md. : 1950),  (15): [PMID:16148104]
36. Carlo-Stella, Carmelo C and 13 more authors..  (2006)  The anti-human leukocyte antigen-DR monoclonal antibody 1D09C3 activates the mitochondrial cell death pathway and exerts a potent antitumor activity in lymphoma-bearing nonobese diabetic/severe combined immunodeficient mice..  Cancer research,  (1): [PMID:16452241]
37. Balas, Antonio A and 7 more authors..  (2006)  Group-specific amplification of cDNA from DRB1 genes. Complete coding sequences of partially defined alleles and identification of the new alleles DRB1*040602, DRB1*111102, DRB1*080103, and DRB1*0113..  Human immunology,  [PMID:17174751]
38. Schmid, Dorothee D, Pypaert, Marc M and Münz, Christian C..  (2007)  Antigen-loading compartments for major histocompatibility complex class II molecules continuously receive input from autophagosomes..  Immunity,  [PMID:17182262]
39. von Salomé, Jenny J, Gyllensten, Ulf U and Bergström, Tomas F TF..  (2007)  Full-length sequence analysis of the HLA-DRB1 locus suggests a recent origin of alleles..  Immunogenetics,  [PMID:17345114]
40. De Gassart, Aude A and 6 more authors..  (2008)  MHC class II stabilization at the surface of human dendritic cells is the result of maturation-dependent MARCH I down-regulation..  Proceedings of the National Academy of Sciences of the United States of America,  (4): [PMID:18305173]
41. Dephoure, Noah N and 6 more authors..  (2008)  A quantitative atlas of mitotic phosphorylation..  Proceedings of the National Academy of Sciences of the United States of America,  (5): [PMID:18669648]
42. Fujiki, Fumihiro F and 15 more authors..  (2008)  A WT1 protein-derived, naturally processed 16-mer peptide, WT1(332), is a promiscuous helper peptide for induction of WT1-specific Th1-type CD4(+) T cells..  Microbiology and immunology,  [PMID:19120973]
43. Harkiolaki, Maria M and 18 more authors..  (2009)  T cell-mediated autoimmune disease due to low-affinity crossreactivity to common microbial peptides..  Immunity,  (20): [PMID:19303388]
44. Stumpf, Anita N AN and 5 more authors..  (2009)  Identification of 4 new HLA-DR-restricted minor histocompatibility antigens as hematopoietic targets in antitumor immunity..  Blood,  (22): [PMID:19706888]
45. Faner, Rosa R and 5 more authors..  (2010)  Reassessing the role of HLA-DRB3 T-cell responses: evidence for significant expression and complementary antigen presentation..  European journal of immunology,  [PMID:19830726]
46. Zhu, F F and 8 more authors..  (2011)  Analysis of the complete cDNA sequences of HLA-DRB1 alleles with group-specific amplification primers in the Chinese Han population..  Tissue antigens,  [PMID:21388356]
47. Wang, Xin Xiang XX and 7 more authors..  (2011)  Affinity maturation of human CD4 by yeast surface display and crystal structure of a CD4-HLA-DR1 complex..  Proceedings of the National Academy of Sciences of the United States of America,  (20): [PMID:21900604]
48. Raychaudhuri, Soumya S and 14 more authors..  (2012)  Five amino acids in three HLA proteins explain most of the association between MHC and seropositive rheumatoid arthritis..  Nature genetics,  (29): [PMID:22286218]
49. Kwok, William W and 8 more authors..  (2012)  Frequency of epitope-specific naive CD4(+) T cells correlates with immunodominance in the human memory repertoire..  Journal of immunology (Baltimore, Md. : 1950),  (15): [PMID:22327072]
50. Anguille, S S and 7 more authors..  (2013)  Identification of a Wilms' tumor 1-derived immunogenic CD4(+) T-cell epitope that is recognized in the context of common Caucasian HLA-DR haplotypes..  Leukemia,  [PMID:22929521]
51. Pos, Wouter W and 6 more authors..  (2012)  Crystal structure of the HLA-DM-HLA-DR1 complex defines mechanisms for rapid peptide selection..  Cell,  (21): [PMID:23260142]
52. Goyette, Philippe P and 32 more authors..  (2015)  High-density mapping of the MHC identifies a shared role for HLA-DRB1*01:03 in inflammatory bowel diseases and heterozygous advantage in ulcerative colitis..  Nature genetics,  [PMID:25559196]
53. Gerritsen, K E H KE, Groeneweg, M M, Meertens, C M H CM, Voorter, C E M CE and Tilanus, M G J MG..  (2015)  Full-length HLA-DRB1 coding sequences generated by a hemizygous RNA-SBT approach..  Tissue antigens,  [PMID:26396036]
54. Muehling, Lyndsey M LM and 5 more authors..  (2016)  Circulating Memory CD4+ T Cells Target Conserved Epitopes of Rhinovirus Capsid Proteins and Respond Rapidly to Experimental Infection in Humans..  Journal of immunology (Baltimore, Md. : 1950),  (15): [PMID:27591323]
55. Wieczorek, Marek M and 6 more authors..  (2017)  Major Histocompatibility Complex (MHC) Class I and MHC Class II Proteins: Conformational Plasticity in Antigen Presentation..  Frontiers in immunology,  [PMID:28367149]
56. Ooi, Joshua D and 24 more authors..  (2017)  Dominant protection from HLA-linked autoimmunity by antigen-specific regulatory T cells..  Nature,  (11): [PMID:28467828]
57. Ting, Yi Tian YT and 10 more authors..  (2018)  The interplay between citrullination and HLA-DRB1 polymorphism in shaping peptide binding hierarchies in rheumatoid arthritis..  The Journal of biological chemistry,  (2): [PMID:29317506]
58. Galperin, Moran M and 11 more authors..  (2018)  CD4+ T cell-mediated HLA class II cross-restriction in HIV controllers..  Science immunology,  (8): [PMID:29884618]
59. Yossef, Rami R and 10 more authors..  (2018)  Enhanced detection of neoantigen-reactive T cells targeting unique and shared oncogenes for personalized cancer immunotherapy..  JCI insight,  (4): [PMID:30282837]
60. Suzuki, Shingo S and 16 more authors..  (2018)  Reference Grade Characterization of Polymorphisms in Full-Length HLA Class I and II Genes With Short-Read Sequencing on the ION PGM System and Long-Reads Generated by Single Molecule, Real-Time Sequencing on the PacBio Platform..  Frontiers in immunology,  [PMID:30337930]
61. Meckiff, Benjamin J and 7 more authors..  (2019)  Primary EBV Infection Induces an Acute Wave of Activated Antigen-Specific Cytotoxic CD4+ T Cells..  Journal of immunology (Baltimore, Md. : 1950),  (1): [PMID:31308093]
62. MacLachlan, Bruce J and 13 more authors..  (2019)  Human leukocyte antigen (HLA) class II peptide flanking residues tune the immunogenicity of a human tumor-derived epitope..  The Journal of biological chemistry,  (27): [PMID:31619516]
63. Greenshields-Watson, Alexander and 25 more authors..  (2020)  CD4+ T Cells Recognize Conserved Influenza A Epitopes through Shared Patterns of V-Gene Usage and Complementary Biochemical Features..  Cell reports,  (14): [PMID:32668259]

溶液计算器