hHR23b Mouse mAb

COA

级别和纯度:

已验证

ExactAb™

1.0 mg/mL

应用:

IF/ICC
IHC
WB

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human), 仓鼠(Hamster), 大鼠(Rat), 小鼠(Mouse), 猴(Monkey)
亚型: Mouse IgG2b
克隆号: 5H1-A10-A7
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab107446-10μl	10μl	现货
Ab107446-50μl	50μL	现货
Ab107446-100μl	100μL	期货
Ab107446-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: RAD23B

Accession#: P54727 (1) Gene ID: 5887 (1) hHR23b (1) RAD23B (1) RD23B (1)

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基本信息

产品名称	hHR23b Mouse mAb
别名	hHR23b抗体 \| 紫外切除修复蛋白RAD23B抗体 \| RAD23同源物B抗体 \| hHR23b小鼠单克隆抗体 \| hHR23b小鼠单抗
英文别名	hHR23B \| HR23B \| p58 \| XP-C repair-complementing complex 58 kDa protein \| hHR 23b antibody \| hHR23B antibody \| HR 23B antibody \| HR23B antibody \| mHR 23B antibody \| mHR23B antibody \| p58 antibody \| RAD 23B antibody \| RAD23 (S. cerevisiae) homolog B antibo
规格或纯度	ExactAb™, Validated, 1.0 mg/mL
宿主种属	小鼠(Mouse)
特异性	hHR23b
种属反应性	人(Human),仓鼠(Hamster),大鼠(Rat),小鼠(Mouse),猴(Monkey)
免疫原	Recombinant protein of human hHR23b (AA 1-409)
阳性对照	WB: HeLa, K562, MCF7, NIH/3T3 and C6 cell lysates. IHC: Prostate cancer tissue. ICC/IF: HeLa cells.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	5H1-A10-A7
Format	Whole IgG
亚型	Mouse IgG2b
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.5% BSA, 0.02% Sodium azide, pH 7.3
防腐剂	0.02% Sodium azide
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

RAD23B Tbio 紫外线切除修复蛋白 RAD23 同源物 B(UV excision repair protein RAD23 homolog B) (0 活性数据)

点击查看靶蛋白信息： RAD23B

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: hHR23b Mouse mAb (Ab107446)

hHR23b Mouse mAb (Ab107446) - Western Blot
All lanes: hHR23b Mouse mAb (Ab107446) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 54 kDa
Exposure time: 5.2 s

IF/ICC: hHR23b Mouse mAb (Ab107446)

hHR23b Mouse mAb (Ab107446) - IF/ICC
Immunocytochemistry analysis of Rad23B (5H1) in HeLa using hHR23b Mouse mAb (Ab107446).

IHC: hHR23b Mouse mAb (Ab107446)

hHR23b Mouse mAb (Ab107446) - IHC
Immunohistochemistry analysis of paraffin-embedded Prostate Cancer using hHR23b Mouse mAb (Ab107446). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

应用

应用名称	稀释比例
WB	1/500-1/1000
IHC	1/50 - 1/100
IF/ICC	1/50-1/200

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到2个结果

批号(Lot Number)	证书类型	货号
ZJ24F0708933	分析证书	Ab107446
ZJ24F0708932	分析证书	Ab107446

引用文献

1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
2. Masutani, C C and 9 more authors.. (1994) Purification and cloning of a nucleotide excision repair complex involving the xeroderma pigmentosum group C protein and a human homologue of yeast RAD23.. The EMBO journal, (15): [PMID:8168482]
3. Sugasawa, K K and 13 more authors.. (1997) Two human homologs of Rad23 are functionally interchangeable in complex formation and stimulation of XPC repair activity.. Molecular and cellular biology, [PMID:9372924]
4. Sugasawa, K K and 8 more authors.. (1998) Xeroderma pigmentosum group C protein complex is the initiator of global genome nucleotide excision repair.. Molecular cell, [PMID:9734359]
5. Hiyama, H H and 7 more authors.. (1999) Interaction of hHR23 with S5a. The ubiquitin-like domain of hHR23 mediates interaction with S5a subunit of 26 S proteasome.. The Journal of biological chemistry, (24): [PMID:10488153]
6. Batty, D D, Rapic'-Otrin, V V, Levine, A S AS and Wood, R D RD.. (2000) Stable binding of human XPC complex to irradiated DNA confers strong discrimination for damaged sites.. Journal of molecular biology, (7): [PMID:10873465]
7. Wang, G G, Sawai, N N, Kotliarova, S S, Kanazawa, I I and Nukina, N N.. (2000) Ataxin-3, the MJD1 gene product, interacts with the two human homologs of yeast DNA repair protein RAD23, HHR23A and HHR23B.. Human molecular genetics, (22): [PMID:10915768]
8. Araki, M M and 7 more authors.. (2001) Centrosome protein centrin 2/caltractin 1 is part of the xeroderma pigmentosum group C complex that initiates global genome nucleotide excision repair.. The Journal of biological chemistry, (1): [PMID:11279143]
9. Uchida, Akio A and 7 more authors.. (2002) The carboxy-terminal domain of the XPC protein plays a crucial role in nucleotide excision repair through interactions with transcription factor IIH.. DNA repair, (21): [PMID:12509233]
10. Sugasawa, Kaoru K, Shimizu, Yuichiro Y, Iwai, Shigenori S and Hanaoka, Fumio F.. (2002) A molecular mechanism for DNA damage recognition by the xeroderma pigmentosum group C protein complex.. DNA repair, (22): [PMID:12509299]
11. Ng, Jessica M Y JM and 6 more authors.. (2003) A novel regulation mechanism of DNA repair by damage-induced and RAD23-dependent stabilization of xeroderma pigmentosum group C protein.. Genes & development, (1): [PMID:12815074]
12. Ryu, Kyoung-Seok KS and 5 more authors.. (2003) Binding surface mapping of intra- and interdomain interactions among hHR23B, ubiquitin, and polyubiquitin binding site 2 of S5a.. The Journal of biological chemistry, (19): [PMID:12832454]
13. Fujiwara, Kenichiro K and 9 more authors.. (2004) Structure of the ubiquitin-interacting motif of S5a bound to the ubiquitin-like domain of HR23B.. The Journal of biological chemistry, (6): [PMID:14585839]
14. Huang, Xiaoyan X and 7 more authors.. Expression of a novel RAD23B mRNA splice variant in the human testis.. Journal of andrology, [PMID:15064313]
15. Humphray, S J SJ and 147 more authors.. (2004) DNA sequence and analysis of human chromosome 9.. Nature, (27): [PMID:15164053]
16. Katiyar, Samiksha S, Li, Guangtao G and Lennarz, William J WJ.. (2004) A complex between peptide:N-glycanase and two proteasome-linked proteins suggests a mechanism for the degradation of misfolded glycoproteins.. Proceedings of the National Academy of Sciences of the United States of America, (21): [PMID:15358861]
17. Kim, Byoungkook B, Ryu, Kyoung-Seok KS, Kim, Hyun-Jin HJ, Cho, Sung-Jae SJ and Choi, Byong-Seok BS.. (2005) Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B.. The FEBS journal, [PMID:15885096]
18. Nishi, Ryotaro R and 7 more authors.. (2005) Centrin 2 stimulates nucleotide excision repair by interacting with xeroderma pigmentosum group C protein.. Molecular and cellular biology, [PMID:15964821]
19. Katiyar, Samiksha S and Lennarz, William J WJ.. (2005) Studies on the intracellular localization of hHR23B.. Biochemical and biophysical research communications, (2): [PMID:16253613]
20. Chen, Li L and Madura, Kiran K.. (2006) Evidence for distinct functions for human DNA repair factors hHR23A and hHR23B.. FEBS letters, (12): [PMID:16712842]
21. Wang, Xiaorong X and 5 more authors.. (2007) Mass spectrometric characterization of the affinity-purified human 26S proteasome complex.. Biochemistry, (20): [PMID:17323924]
22. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648]
23. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976]
24. Li, Xiaohua X and Demartino, George N GN.. (2009) Variably modulated gating of the 26S proteasome by ATP and polyubiquitin.. The Biochemical journal, (15): [PMID:19435460]
25. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]
26. Sugasawa, Kaoru K, Akagi, Jun-ichi J, Nishi, Ryotaro R, Iwai, Shigenori S and Hanaoka, Fumio F.. (2009) Two-step recognition of DNA damage for mammalian nucleotide excision repair: Directional binding of the XPC complex and DNA strand scanning.. Molecular cell, (25): [PMID:19941824]
27. Neher, Tracy M TM, Rechkunova, Nadejda I NI, Lavrik, Olga I OI and Turchi, John J JJ.. (2010) Photo-cross-linking of XPC-Rad23B to cisplatin-damaged DNA reveals contacts with both strands of the DNA duplex and spans the DNA adduct.. Biochemistry, (2): [PMID:20028083]
28. Shimizu, Yuichiro and 5 more authors.. (2010) Stimulation of DNA Glycosylase Activities by XPC Protein Complex: Roles of Protein-Protein Interactions.. Journal of nucleic acids, (25): [PMID:20798892]
29. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692]
30. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
31. Weishäupl, Daniel D and 9 more authors.. (2019) Physiological and pathophysiological characteristics of ataxin-3 isoforms.. The Journal of biological chemistry, (11): [PMID:30455355]
32. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

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所需的复溶浓度

hHR23b Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器