Recombinant EpCAM Antibody

COA

级别和纯度:

0.5 mg/mL

应用:

Flow Cytometry
IF/ICC
IHC
IP
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human)
亚型: Rabbit IgG
克隆号: RR694
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab101839-10μl	10μl	现货
Ab101839-50μl	50μL	现货
Ab101839-100μl	100μL	现货
Ab101839-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: EPCAM

Accession#:P16422 (16) Epcam (20) Gene ID:4072 (17) 流式细胞术 (1163)

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基本信息

产品名称	Recombinant EpCAM Antibody
别名	上皮特异性抗原抗体 \| anti- EpCAM抗体 \| 上皮细胞粘附分子抗体 \| 重组EpCAM抗体
英文别名	17 1A antibody \| 323/A3 antibody \| Adenocarcinoma associated antigen antibody \| Adenocarcinoma-associated antigen antibody \| Antigen identified by monoclonal antibody AUA1 antibody \| AUA1 antibody \| CD326 antibody \| CD326 antigen antibody \| Cell surface g
规格或纯度	ExactAb™, Validated, Recombinant, High performance, 0.5 mg/mL
宿主种属	兔(Rabbit)
特异性	EpCAM
种属反应性	人(Human)
免疫原	A synthetic peptide corresponding to residues of human EPCAM. (AA 1-100)
阳性对照	WB: Hep G2, HEK293 and A431 cell lysates. IHC: Human Uterus tissue. Flow: HCT-116 cells. IP: HT-29 whole cell lysate. IF/ICC: HT-29 cells.
偶联	Unconjugated

产品属性

克隆类型	重组抗体
克隆号	RR694
Format	Whole IgG
亚型	Rabbit IgG
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	59% 10 mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3.
防腐剂	0.01% Sodium azide
浓度	0.5 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

EPCAM Tbio 上皮细胞粘附分子(Epithelial cell adhesion molecule) (0 活性数据)

点击查看靶蛋白信息： EPCAM

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: Recombinant EpCAM Antibody (Ab101839)

Recombinant EpCAM Antibody (Ab101839) - Western Blot
All lanes: Recombinant EpCAM Antibody (Ab101839) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 40 kDa
Observed band size: 39 kDa
Exposure time: 5.0 s

IHC: Recombinant EpCAM Antibody (Ab101839)

Recombinant EpCAM Antibody (Ab101839) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus tissue labelling EpCAM with Recombinant EpCAM Antibody (Ab101839) at 1/3200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Flow Cytometry: Recombinant EpCAM Antibody (Ab101839)

Recombinant EpCAM Antibody (Ab101839) - Flow Cytometry
Overlay histogram showing HCT-116 cells stained with Recombinant EpCAM Antibody (Ab101839) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant EpCAM Antibody (Ab101839) ( 1/800 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit AF488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.

IP: Recombinant EpCAM Antibody (Ab101839)

Recombinant EpCAM Antibody (Ab101839) - IP
EpCAM was immunoprecipitated from 0.4 mg of HT-29 whole cell lysate with Recombinant EpCAM Antibody (Ab101839) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500

Lane 1: Recombinant EpCAM Antibody (Ab101839) IP in HT-29 whole cell lysate
Lane 2: PBS instead of Recombinant EpCAM Antibody (Ab101839) in HT-29 whole cell lysate
Lane 3: HT-29 whole cell lysate, 10 μg (input)

Exposure: 120 s

IF: Recombinant EpCAM Antibody (Ab101839)

Recombinant EpCAM Antibody (Ab101839) - IF
IF analysis of EpCAM (green) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant EpCAM Antibody (Ab101839) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (LumiDye™ Blue 515) (Ab220504) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

应用

应用名称	稀释比例
WB	1/2000 - 1/5000
Flow Cytometry	1/800 - 1/2000
IP	1/50
IHC	1/1600-1/3200
IF/ICC	1/1000 - 1/2000

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通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

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批号(Lot Number)	证书类型	货号
ZJ23F0901017	分析证书	Ab101839
ZJ23F0901018	分析证书	Ab101839
ZJ24F0911363	分析证书	Ab101839

技术文档和文章

流式细胞术

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流式细胞术

流式细胞仪分析

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前向散射侧向散射二向色滤光片多色流式细胞仪

流式细胞术实验方案

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抗体流式细胞术流式抗体活性染料间接抗体标记

免疫沉淀(IP)裂解液和试剂

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免疫沉淀裂解液组织裂解物

冷冻样本的免疫组织化学染色实验方案（IHC-Fr）

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重组抗体单克隆抗体 IHC 免疫组化冷冻切片

免疫组织化学染色的抗原修复方案

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免疫沉淀通用实验方案

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免疫沉淀蛋白质印迹细胞裂解液免疫共沉淀 IP 蛋白质互作蛋白A/G磁珠

General Immunoprecipitation Protocol

Categories: 实验方案（Protocols）

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Immunoprecipitation Western blot Cell lysate IP Co-immunoprecipitation Protein interaction Protein A/G magnetic beads

General Immunoprecipitation Protocol

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Immunoprecipitation Western blot Cell lysate IP Co-immunoprecipitation Protein interaction Protein A/G magnetic beads

General Immunoprecipitation Protocol

Categories: 实验方案（Protocols）

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Immunoprecipitation Western blot Cell lysate IP Co-immunoprecipitation Protein interaction Protein A/G magnetic beads

General Immunoprecipitation Protocol

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Immunoprecipitation Western blot Cell lysate IP Co-immunoprecipitation Protein interaction Protein A/G magnetic beads

RNA免疫沉淀方案

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交联 IP 蛋白A/G磁珠 RNA免疫沉淀 RIP RNA蛋白质互作同种型对照抗体 Input

RNA Immunoprecipitation (RIP) Protocol

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IP Protein A/G magnetic beads RIP Input RNA immunoprecipitation RNA protein interaction Isotype control antibody Crosslinking

RNA Immunoprecipitation (RIP) Protocol

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IP Protein A/G magnetic beads RIP Input RNA immunoprecipitation RNA protein interaction Isotype control antibody Crosslinking

固定免疫沉淀实验方案

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免疫沉淀蛋白质印迹 WB IP 蛋白质互作固定免疫沉淀固定化抗体

Immobilized IP Protocol

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Immunoprecipitation WB Western blot IP Protein interaction Immobilization immunoprecipitation Immobilized antibody

Immobilized IP Protocol

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Immunoprecipitation WB Western blot IP Protein interaction Immobilization immunoprecipitation Immobilized antibody

利用甲醇通透法进行流式细胞术

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流式细胞术流式抗体流式实验甲醇通透细胞分选细胞分型免疫研究细胞内抗原检测

Flow Cytometry Using Methanol Permeabilization

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Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

Flow Cytometry Using Methanol Permeabilization

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引用文献

1. Simon, B B and 5 more authors.. (1990) Epithelial glycoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2108441]
2. Szala, S S and 6 more authors.. (1990) Molecular cloning of cDNA for the carcinoma-associated antigen GA733-2.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2333300]
3. Strnad, J J and 9 more authors.. (1989) Molecular cloning and characterization of a human adenocarcinoma/epithelial cell surface antigen complementary DNA.. Cancer research, (15): [PMID:2463074]
4. Perez, M S MS and Walker, L E LE.. (1989) Isolation and characterization of a cDNA encoding the KS1/4 epithelial carcinoma marker.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:2469722]
5. Linnenbach, A J AJ and 7 more authors.. (1989) Sequence investigation of the major gastrointestinal tumor-associated antigen gene family, GA733.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2911574]
6. Björk, P P and 8 more authors.. (1993) Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody.. The Journal of biological chemistry, (15): [PMID:7693697]
7. Linnenbach, A J AJ and 7 more authors.. (1993) Retroposition in a family of carcinoma-associated antigen genes.. Molecular and cellular biology, [PMID:8382772]
8. Chong, J M JM and Speicher, D W DW.. (2001) Determination of disulfide bond assignments and N-glycosylation sites of the human gastrointestinal carcinoma antigen GA733-2 (CO17-1A, EGP, KS1-4, KSA, and Ep-CAM).. The Journal of biological chemistry, (23): [PMID:11080501]
9. Münz, Markus M and 5 more authors.. (2004) The carcinoma-associated antigen EpCAM upregulates c-myc and induces cell proliferation.. Oncogene, (29): [PMID:15195135]
10. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621]
11. Münz, Markus M, Zeidler, Reinhard R and Gires, Olivier O.. (2005) The tumour-associated antigen EpCAM upregulates the fatty acid binding protein E-FABP.. Cancer letters, (8): [PMID:15922867]
12. Ladwein, Markus M and 8 more authors.. (2005) The cell-cell adhesion molecule EpCAM interacts directly with the tight junction protein claudin-7.. Experimental cell research, (1): [PMID:16054130]
13. Munz, Markus M, Fellinger, Karin K, Hofmann, Tanja T, Schmitt, Barbel B and Gires, Olivier O.. (2008) Glycosylation is crucial for stability of tumour and cancer stem cell antigen EpCAM.. Frontiers in bioscience : a journal and virtual library, (1): [PMID:18508581]
14. Sivagnanam, Mamata M and 15 more authors.. (2008) Identification of EpCAM as the gene for congenital tufting enteropathy.. Gastroenterology, [PMID:18572020]
15. Ligtenberg, Marjolijn J L MJ and 17 more authors.. (2009) Heritable somatic methylation and inactivation of MSH2 in families with Lynch syndrome due to deletion of the 3' exons of TACSTD1.. Nature genetics, [PMID:19098912]
16. Ng, Valerie Y VY, Ang, Sheu Ngo SN, Chan, Jia Xin JX and Choo, Andre B H AB.. (2010) Characterization of epithelial cell adhesion molecule as a surface marker on undifferentiated human embryonic stem cells.. Stem cells (Dayton, Ohio), [PMID:19785009]
17. Lu, Tung-Ying TY and 6 more authors.. (2010) Epithelial cell adhesion molecule regulation is associated with the maintenance of the undifferentiated phenotype of human embryonic stem cells.. The Journal of biological chemistry, (19): [PMID:20064925]
18. Seimetz, Diane D, Lindhofer, Horst H and Bokemeyer, Carsten C.. (2010) Development and approval of the trifunctional antibody catumaxomab (anti-EpCAM x anti-CD3) as a targeted cancer immunotherapy.. Cancer treatment reviews, [PMID:20347527]
19. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

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体积（添加到小瓶中）

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所需的复溶浓度

Recombinant EpCAM Antibody

库存信息

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库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器