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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab138205-100μl |
100μL |
现货 ![]() |
| |
| Ab138205-1ml |
1ml |
现货 ![]() |
|
| 产品名称 | Goat Anti-Rabbit IgG H&L (AF488) |
|---|---|
| 英文别名 | Goat Anti-Rabbit IgG H&L (AF488) | Goat Anti Rabbit IgG H&L (AF488) | Goat Anti-Rabbit IgG H&L-AF488 | Goat Anti Rabbit IgG H&L-AF488 | AF488-Goat Anti-Rabbit IgG H&L | AF488-Goat Anti Rabbit IgG H&L |
| 规格或纯度 | ExactAb™, 高性能, 已验证, Ex:490nm, Em:525nm, 1 mg/mL |
| 宿主种属 | 山羊(Goat) |
| 种属反应性 | 兔(Rabbit) |
| 免疫原 | Rabbit IgG |
| 偶联 | AF488 |
| Excitation(nm) | Blue Laser(488nm) |
| Emission(nm) | 525nm |
| Ex/Em(nm) | Ex:490nm;Em:525nm |
| 克隆类型 | 多克隆抗体 |
|---|---|
| Format | Whole IgG |
| 亚型 | IgG |
| 纯化方法 | Antigen Affinity Purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, 50% Glycerol, 1.0% BSA, 0.02% Sodium azide, pH 7.2 |
| 防腐剂 | 0.02% Sodium Azide |
| 浓度 | 1 mg/mL |
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 短期储存于 4°C。分装后在 -20°C 长期保存。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
Goat Anti-Rabbit IgG H&L (AF488) (Ab138205) - ICC/IF
IF analysis of HeLa cells labeling PABPN1 with purified PABPN1 Rabbit mAb (Ab119979) at 1/200 dilution. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were counterstained with beta Actin Mouse mAb (Ab155822) at 1/500 dilution - Goat Anti-Mouse IgG H&L (AF647) 1/1000. Goat Anti-Rabbit IgG H&L (AF488) (Ab138205) was used as the secondary antibody at 1/500 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.
| 应用名称 | 稀释比例 |
|---|---|
| ELISA |
1/5000 - 1/20000
|
| IF/ICC |
1/100-1/1000
|
| Flow Cytometry |
1/500-1/1000
|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab138205 | |
| 分析证书 | Ab138205 | |
| 分析证书 | Ab138205 |
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