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Goat Anti-Rabbit IgG H&L (AF488)

    应用:
  • ELISA
  • Flow Cytometry
  • IF/ICC
  • 宿主种属: 山羊(Goat)
  • 种属反应性: 兔(Rabbit)
  • 亚型: Goat IgG
  • 偶联: AF488
有货

库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
Ab138205-100μl
100μL 现货 Stock Image
Ab138205-1ml
1ml 现货 Stock Image

基本信息

产品名称 Goat Anti-Rabbit IgG H&L (AF488)
英文别名 Goat Anti-Rabbit IgG H&L (AF488) | Goat Anti Rabbit IgG H&L (AF488) | Goat Anti-Rabbit IgG H&L-AF488 | Goat Anti Rabbit IgG H&L-AF488 | AF488-Goat Anti-Rabbit IgG H&L | AF488-Goat Anti Rabbit IgG H&L
规格或纯度 ExactAb™, 高性能, 已验证, Ex:490nm, Em:525nm, 1 mg/mL
宿主种属 山羊(Goat)
种属反应性 兔(Rabbit)
免疫原 Rabbit IgG
偶联 AF488
Excitation(nm) Blue Laser(488nm)
Emission(nm) 525nm
Ex/Em(nm) Ex:490nm;Em:525nm

产品属性

克隆类型 多克隆抗体
Format Whole IgG
亚型 IgG
纯化方法 Antigen Affinity Purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 1.0% BSA, 0.02% Sodium azide, pH 7.2
防腐剂 0.02% Sodium Azide
浓度 1 mg/mL
储存温度 -20°C储存
运输条件 超低温冰袋运输
稳定性与储存 短期储存于 4°C。分装后在 -20°C 长期保存。避免冷冻/解冻循环。
分子类型 抗体

图片

Goat Anti-Rabbit IgG H&L (AF488) (Ab138205) - ICC/IF
IF analysis of HeLa cells labeling PABPN1 with purified PABPN1 Rabbit mAb (Ab119979) at 1/200 dilution. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were counterstained with beta Actin Mouse mAb (Ab155822) at 1/500 dilution - Goat Anti-Mouse IgG H&L (AF647) 1/1000. Goat Anti-Rabbit IgG H&L (AF488) (Ab138205) was used as the secondary antibody at 1/500 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

应用

应用名称 稀释比例
ELISA

1/5000 - 1/20000

IF/ICC

1/100-1/1000

Flow Cytometry

1/500-1/1000

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ23F0700536 分析证书 Ab138205
ZJ23F0700535 分析证书 Ab138205
ZJ25F0622519 分析证书 Ab138205

技术文档和文章

抗体结构和亚型
流式细胞术
流式细胞仪分析
如何选择和使用抗体 — 一抗
How to choose and use antibodies-primary antibody?
如何找到合适的二抗?
抗体应用和技术
Antibody applications and techniques
Antibody applications and techniques
流式细胞术实验方案
How to choose and use antibodies-primary antibody?
Antibody applications and techniques
Antibody applications and techniques
How to choose and use antibodies-primary antibody?
免疫细胞化学染色实验方案
冷冻样本的免疫组织化学染色实验方案(IHC-Fr)
RNA斑点杂交实验方案
RNA dot blot protocol
免疫组织化学染色的抗原修复方案
RNA dot blot protocol
Caspase免疫荧光染色方案
Caspase Immunofluorescence Staining Protocol
Caspase Immunofluorescence Staining Protocol
蛋白质印迹通用实验方案
General Protocol for Western Blotting
General Protocol for Western Blotting
General Protocol for Western Blotting
General Protocol for Western Blotting
General Protocol for Western Blotting
General Protocol for Western Blotting
荧光标记抗体的蛋白质印迹法
Western Blotting with Fluorescently Labeled Antibodies
Western Blotting with Fluorescently Labeled Antibodies
General Protocol for Western Blotting
免疫荧光染色实验步骤(甲醇通透法)
Immunofluorescence Staining Protocol (Methanol permeabilization)
Immunofluorescence Staining Protocol (Methanol permeabilization)
How to choose and use antibodies-primary antibody?
蛋白质印迹的二抗选择指南
利用甲醇通透法进行流式细胞术
Flow Cytometry Using Methanol Permeabilization
Flow Cytometry Using Methanol Permeabilization
CLARITY组织透明化染色技术
转录因子流式细胞术的固定和通透方案
Fixation and Permeabilization Protocol for Transcription Factor Flow Cytometry
Fixation and Permeabilization Protocol for Transcription Factor Flow Cytometry
Fixation and Permeabilization Protocol for Transcription Factor Flow Cytometry

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